Invitrogen

SNRPB Monoclonal Antibody (Y12)

SNRPB Antibody in Immunocytochemistry (ICC/IF)

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SNRPB Antibody (MA5-13449) in ICC/IF

Immunofluorescence analysis of SNRPB was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with SNRPB Mouse monoclonal antibody (Product # MA5-13449) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at... View More

SNRPB Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SNRPB Antibody in RNA Immunoprecipitation (RIP)

SNRPB Antibody in Immunohistochemistry (Frozen) (IHC (F))

SNRPB Antibody in Western Blot, Immunohistochemistry (WB, IHC)

SNRPB Antibody in Immunoprecipitation (IP)

Product Image Gallery

SNRPB Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with SNRPB siRNA and reduction of specific signal was observed in immunofluorescence application using SNRPB Mouse Monoclonal antibody (Product # MA5-13449). {KD}

Product Details

MA5-13449

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-1.0 µg/mL

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2 µg/mL

View 7 publications 7 publications

Immunoprecipitation (IP)

View 8 publications 8 publications

in situ PLA (PLA)

View 1 publication 1 publication

Immunomicroscopy (IM)

Assay-dependent

RNA Immunoprecipitation (RIP)

1.5 µg/10^6 cells

Product Specifications

Species Reactivity

Fruit fly, Human, Mouse, Pig, Rabbit, Rat

Published species

Chicken, Dog, Flatworm, Fruit fly, Hamster, Human, Mouse, Rat

Host/Isotype

Mouse / IgG3, kappa

Class

Monoclonal

Type

Antibody

Clone

Y12

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

200 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10944191

Target Information

The protein encoded by this gene is one of several nuclear proteins that are found in common among U1, U2, U4/U6, and U5 small ribonucleoprotein particles (snRNPs). These snRNPs are involved in pre-mRNA splicing, and the encoded protein may also play a role in pre-mRNA splicing or snRNP structure. Autoantibodies from patients with systemic lupus erythematosus frequently recognize epitopes on the encoded protein. Two transcript variants encoding different isoforms (B and B') have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Non-ubiquitous expression of core spliceosomal protein SmB/B' in chick and mouse embryos.

Developmental dynamics : an official publication of the American Association of Anatomists

Turner BRH,Mellor C,McElroy C,Bowen N,Gu W,Knill C,Itasaki N

MA5-13449 was used in Immunohistochemistry to investigate the expression pattern of a core protein of the major spliceosome, SmB/B' (Small Nuclear Ribonucleoprotein Polypeptides B/B'), which is encoded by SNRPB.

Wed Feb 01 00:00:00 UTC 2023

The integrity of the U12 snRNA 3' stem-loop is necessary for its overall stability.

Nucleic acids research

Norppa AJ,Frilander MJ

Published figure using SNRPB monoclonal antibody (Product # MA5-13449) in Immunoprecipitation

Thu Mar 18 00:00:00 UTC 2021

Therapeutic manipulation of IKBKAP mis-splicing with a small molecule to cure familial dysautonomia.

Nature communications

Ajiro M,Awaya T,Kim YJ,Iida K,Denawa M,Tanaka N,Kurosawa R,Matsushima S,Shibata S,Sakamoto T,Studer L,Krainer AR,Hagiwara M

MA5-13449 was used in Immunocytochemistry to indicate that small synthetic molecules affecting phosphorylation state of SRSFs is available as a new therapeutic modality for mechanism-oriented precision medicine of splicing diseases.

Fri Jul 23 00:00:00 UTC 2021

Therapeutic manipulation of IKBKAP mis-splicing with a small molecule to cure familial dysautonomia.

Nature communications

Ajiro M,Awaya T,Kim YJ,Iida K,Denawa M,Tanaka N,Kurosawa R,Matsushima S,Shibata S,Sakamoto T,Studer L,Krainer AR,Hagiwara M

Fri Jul 23 00:00:00 UTC 2021

Human spliceosomal snRNA sequence variants generate variant spliceosomes.

RNA (New York, N.Y.)

Mabin JW,Lewis PW,Brow DA,Dvinge H

MA5-13449 was used in Immunoprecipitation to investigate if variant spliceosomes containing noncanonical snRNAs may contribute to different tissue-and cell-type-specific alternative splicing patterns.

Fri Oct 01 00:00:00 UTC 2021

PRMT1 loss sensitizes cells to PRMT5 inhibition.

Nucleic acids research

Gao G,Zhang L,Villarreal OD,He W,Su D,Bedford E,Moh P,Shen J,Shi X,Bedford MT,Xu H

MA5-13449 was used in Western Blotting to study the PRMT5 signalling pathway and genes whose loss of function sensitises cancer cells to PRMT5 inhibition.

Tue Jun 04 00:00:00 UTC 2019

PRMT1 loss sensitizes cells to PRMT5 inhibition.

Nucleic acids research

Gao G,Zhang L,Villarreal OD,He W,Su D,Bedford E,Moh P,Shen J,Shi X,Bedford MT,Xu H

MA5-13449 was used in Western Blotting to study the PRMT5 signalling pathway and genes whose loss of function sensitises cancer cells to PRMT5 inhibition.

Tue Jun 04 00:00:00 UTC 2019

Tau-Mediated Disruption of the Spliceosome Triggers Cryptic RNA Splicing and Neurodegeneration in Alzheimer's Disease.

Cell reports

Hsieh YC,Guo C,Yalamanchili HK,Abreha M,Al-Ouran R,Li Y,Dammer EB,Lah JJ,Levey AI,Bennett DA,De Jager PL,Seyfried NT,Liu Z,Shulman JM

Published figure using SNRPB monoclonal antibody (Product # MA5-13449) in Immunohistochemistry

Tue Oct 08 00:00:00 UTC 2019

Tau-Mediated Disruption of the Spliceosome Triggers Cryptic RNA Splicing and Neurodegeneration in Alzheimer's Disease.

Cell reports

Hsieh YC,Guo C,Yalamanchili HK,Abreha M,Al-Ouran R,Li Y,Dammer EB,Lah JJ,Levey AI,Bennett DA,De Jager PL,Seyfried NT,Liu Z,Shulman JM

Published figure using SNRPB monoclonal antibody (Product # MA5-13449) in Immunohistochemistry

Tue Oct 08 00:00:00 UTC 2019

Disrupted alternative splicing for genes implicated in splicing and ciliogenesis causes PRPF31 retinitis pigmentosa.

Nature communications

Buskin A,Zhu L,Chichagova V,Basu B,Mozaffari-Jovin S,Dolan D,Droop A,Collin J,Bronstein R,Mehrotra S,Farkas M,Hilgen G,White K,Pan KT,Treumann A,Hallam D,Bialas K,Chung G,Mellough C,Ding Y,Krasnogor N,Przyborski S,Zwolinski S,Al-Aama J,Alharthi S,Xu Y,Wheway G,Szymanska K,McKibbin M,Inglehearn CF,Elliott DJ,Lindsay S,Ali RR,Steel DH,Armstrong L,Sernagor E,Urlaub H,Pierce E,Lührmann R,Grellscheid SN,Johnson CA,Lako M

MA5-13449 was used in Immunocytochemistry to study the occurrence of disrupted alternative splicing for specific splicing programmes.

Fri Oct 12 00:00:00 UTC 2018

Checkpoints for Autoreactive B Cells in the Peripheral Blood of Lupus Patients Assessed by Flow Cytometry.

Arthritis & rheumatology (Hoboken, N.J.)

Malkiel S,Jeganathan V,Wolfson S,Manjarrez Orduño N,Marasco E,Aranow C,Mackay M,Gregersen PK,Diamond B

MA513449 was used in western blot to develop a method to detect circulating B cells that recognize nuclear antigens in patients

Thu Sep 01 00:00:00 UTC 2016

An Animal Model Using Metallic Ions to Produce Autoimmune Nephritis.

Journal of immunology research

Ramírez-Sandoval R,Luévano-Rodríguez N,Rodríguez-Rodríguez M,Pérez-Pérez ME,Saldívar-Elias S,Gurrola-Carlos R,Avalos-Díaz E,Bollain-y-Goytia JJ,Herrera-Esparza R

MA5-13449 was used in immunohistochemistry - frozen section to investigate the role of metallic ions on induction of experimental autoimmune nephritis

Thu Mar 03 00:00:00 UTC 2016

In situ hybridization protocol for enhanced detection of gene expression in the planarian Schmidtea mediterranea.

BMC developmental biology

King RS,Newmark PA

MA5-13449 was used in Immunocytochemistry-immunoflourescence to optimise the signal intensity and sensitivity in whole-mount in situ hybridisation and whole-mount fluorescent in situ hybridisation in planarians.

Tue Mar 12 00:00:00 UTC 2013

Mutation of a U2 snRNA gene causes global disruption of alternative splicing and neurodegeneration.

Cell

Jia Y,Mu JC,Ackerman SL

MA5-13449 was used in immunoprecipitation to study the role of a mutation of a U2 snRNA gene in global disruption of alternative splicing and neurodegeneration

Fri Jan 20 00:00:00 UTC 2012

Combination of SMN trans-splicing and a neurotrophic factor increases the life span and body mass in a severe model of spinal muscular atrophy.

Human gene therapy

Shababi M,Glascock J,Lorson CL

MA5-13449 was used in immunoprecipitation to investigate the effectiveness of SMN1 gene therapy in the treatment of spinal muscular atrophy in a mouse model

Tue Feb 01 00:00:00 UTC 2011

Functional association of the Microprocessor complex with the spliceosome.

Molecular and cellular biology

Kataoka N,Fujita M,Ohno M

MA5-13449 was used in immunoprecipitation to investigate the molecular basis for intronic miRNA processing

Mon Jun 01 00:00:00 UTC 2009

The inability of fully grown germinal vesicle stage oocyte cytoplasm to transcriptionally silence transferred transcribing nuclei.

Histochemistry and cell biology

Fulka H,Novakova Z,Mosko T,Fulka J

MA5-13449 was used in immunocytochemistry to evaluate the utility of fully grown germinal vesicle stage oocyte cytoplasm in somatic cell nuclear transfer experiments

Thu Oct 01 00:00:00 UTC 2009

Restoration of SMN function: delivery of a trans-splicing RNA re-directs SMN2 pre-mRNA splicing.

Molecular therapy : the journal of the American Society of Gene Therapy

Coady TH,Shababi M,Tullis GE,Lorson CL

MA5-13449 was used in immunoprecipitation to study the restoration of survival motor neuron function by a redirection of SMN2 pre-mRNA splicing

Wed Aug 01 00:00:00 UTC 2007

Arginine methyltransferase Capsuleen is essential for methylation of spliceosomal Sm proteins and germ cell formation in Drosophila.

Development (Cambridge, England)

Anne J,Ollo R,Ephrussi A,Mechler BM

MA5-13449 was used in immunocytochemistry to study the essential role of protein arginine methyltransferase in protein localization in germ cells

Mon Jan 01 00:00:00 UTC 2007

Mutations in splicing factor PRPF3, causing retinal degeneration, form detrimental aggregates in photoreceptor cells.

Human molecular genetics

Comitato A,Spampanato C,Chakarova C,Sanges D,Bhattacharya SS,Marigo V

MA5-13449 was used in immunohistochemistry to investigate the role of mutations in splicing factor PRPF3 in retinal degeneration of photoreceptor cells

Sun Jul 15 00:00:00 UTC 2007

Myosin16b: The COOH-tail region directs localization to the nucleus and overexpression delays S-phase progression.

Cell motility and the cytoskeleton

Cameron RS,Liu C,Mixon AS,Pihkala JP,Rahn RJ,Cameron PL

MA5-13449 was used in immunohistochemistry to study the role of the myosin16b COOH-tail region in localization to the nucleus and S-phase progression

Mon Jan 01 00:00:00 UTC 2007

Gemin8 is required for the architecture and function of the survival motor neuron complex.

The Journal of biological chemistry

Carissimi C,Saieva L,Gabanella F,Pellizzoni L

MA513449 was used in western blot to examine the contribution of Gemin8 to the composition and function of the survival motor neuron protein complex

Fri Dec 01 00:00:00 UTC 2006

Gemin8 is a novel component of the survival motor neuron complex and functions in small nuclear ribonucleoprotein assembly.

The Journal of biological chemistry

Carissimi C,Saieva L,Baccon J,Chiarella P,Maiolica A,Sawyer A,Rappsilber J,Pellizzoni L

MA5-13449 was used in immunoprecipitation to investigate the role of gemin8 in the survival motor neuron complex and its function in small nuclear ribonucleoprotein assembly

Fri Mar 24 00:00:00 UTC 2006

Subcellular localization of protein kinase C delta and epsilon affects transcriptional and post-transcriptional processes in four-cell mouse embryos.

Reproduction (Cambridge, England)

Dehghani H,Reith C,Hahnel AC

MA5-13449 was used in immunohistochemistry to study the role of protein kinase C delta and epsilon subcellular localization on transcriptional and post-transcriptional processes in mouse preimplantation development

Sat Oct 01 00:00:00 UTC 2005

Tim50a, a nuclear isoform of the mitochondrial Tim50, interacts with proteins involved in snRNP biogenesis.

BMC cell biology

Xu H,Somers ZB,Robinson ML,Hebert MD

MA5-13449 was used in immunocytochemistry to study the role of Tim50a in snRNP biogenesis.

Mon Jul 11 00:00:00 UTC 2005

A key role for stress-induced satellite III transcripts in the relocalization of splicing factors into nuclear stress granules.

Journal of cell science

Metz A,Soret J,Vourc'h C,Tazi J,Jolly C

MA5-13449 was used in immunocytochemistry to study the role of stress-induced satellite III transcripts in the relocalization of splicing factors into nuclear stress granules

Wed Sep 01 00:00:00 UTC 2004

ELL and EAF1 are Cajal body components that are disrupted in MLL-ELL leukemia.

Molecular biology of the cell

Polak PE,Simone F,Kaberlein JJ,Luo RT,Thirman MJ

MA5-13449 was used in immunocytochemistry to study the disruption of Cajal body components ELL and EAF1 in MLL-ELL leukemia

Tue Apr 01 00:00:00 UTC 2003

pICln inhibits snRNP biogenesis by binding core spliceosomal proteins.

Molecular and cellular biology

Pu WT,Krapivinsky GB,Krapivinsky L,Clapham DE

MA5-13449 was used in immunoprecipitation to study the mechanism for the inhibitory effect of pICln on snRNP biogenesis

Tue Jun 01 00:00:00 UTC 1999

Bioinformatics

Protein Aliases: B polypeptide of Sm protein; Sm protein B; Sm protein B/B'; Sm-B; Sm-B/B'; sm-B/Sm-B'; SM11; small nuclear ribonucleoprotein polypeptide B; small nuclear ribonucleoprotein polypeptide B/B-like; small nuclear ribonucleoprotein polypeptides B and B'; Small nuclear ribonucleoprotein-associated protein B; Small nuclear ribonucleoprotein-associated proteins B and B'; smB; snRNP-B

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Gene Aliases: AL024368; AU018828; CCMS; COD; SM-B; Sm-B/B'; SM11; SMB; SmB/B'; SmB/SmB'; SNRNP-B; SNRPB; SNRPB1

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UniProt ID: (Human) P14678, (Mouse) P27048, (Rat) P17136

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Entrez Gene ID: (Rabbit) 100341600, (Human) 6628, (Mouse) 20638, (Rat) 171365

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Function(s)

protein binding U2 snRNA binding poly(A) RNA binding telomerase RNA binding histone pre-mRNA DCP binding RNA binding

Process(es)

spliceosomal snRNP assembly mRNA splicing, via spliceosome positive regulation of defense response to virus by host mRNA processing protein methylation RNA splicing mitophagy in response to mitochondrial depolarization termination of RNA polymerase II transcription histone mRNA metabolic process nuclear import xenophagy

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SNRPB Monoclonal Antibody (Y12)

SNRPB Antibody (MA5-13449) in ICC/IF

Product Details

MA5-13449

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

in situ PLA (PLA)

Immunomicroscopy (IM)

RNA Immunoprecipitation (RIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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