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SNRPN Antibody (MA5-49219) in WB
Western blot analysis of SNRPN using SNRPN antibody (Product # MA5-49219). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. Lane 1: human U87 whole cell lysates; Lane 2: human Caco-2 whole cell lysates; Lane 3: rat brain tissue lysates; Lane 4: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the me... View More
Product Details
MA5-49219
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
6F12
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human SNRPN (11-39aa QHIDYRMRCILQDGRIFIGTFKAFDKHMN).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3074877
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Immunogen sequence is identical to the related mouse and rat sequences.
Positive Control - WB: human U87 whole cell, human Caco-2 whole cell, rat brain tissue, mouse brain tissue. Flow: A549 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
The protein encoded by this gene is one polypeptide of a small nuclear ribonucleoprotein complex and belongs to the snRNP SMB/SMN family. The protein plays a role in pre-mRNA processing, possibly tissue-specific alternative splicing events. Although individual snRNPs are believed to recognize specific nucleic acid sequences through RNA-RNA base pairing, the specific role of this family member is unknown. The protein arises from a bicistronic transcript that also encodes a protein identified as the SNRPN upstream reading frame (SNURF). Multiple transcription initiation sites have been identified and extensive alternative splicing occurs in the 5' untranslated region. Additional splice variants have been described but sequences for the complete transcripts have not been determined. The 5' UTR of this gene has been identified as an imprinting center. Alternative splicing or deletion caused by a translocation event in this paternally-expressed region is responsible for Angelman syndrome or Prader-Willi syndrome due to parental imprint switch failure.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp686C0927; DKFZp686M12165; DKFZp761I1912; DKFZp762N022; FE294 gene for snRNP-associated polypeptide N; FE294 psi pseudogene; FLJ33569; FLJ36996; FLJ39265; MGC29886; Sm protein D; SM protein N; Sm-D; Sm-N; small nuclear ribonucleoparticle-associated protein (snRNP) mRNA clone Sm51; small nuclear ribonucleoparticle-associated protein (snRNP) mRNA, clone Sm51; small nuclear ribonucleoprotein N; Small nuclear ribonucleoprotein-associated protein N; smN; snRNP-N; tissue-specific splicing protein; Tissue-specific-splicing protein
Gene Aliases: 2410045I01Rik; FE 294; FE 294 psi; FE294; FE294 psi; HCERN3; Peg4; PWCR; Pwcr1; RT-LI; SM-D; sm-N; SMN; SNRNP-N; SNRPN; SNURF-SNRPN
UniProt ID: (Human) P63162, (Rat) P63164, (Mouse) P63163
Entrez Gene ID: (Human) 6638, (Rat) 81781, (Mouse) 20646
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