Invitrogen

SNTB2 Monoclonal Antibody (1351)

SNTB2 Antibody in Immunocytochemistry (ICC/IF)

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SNTB2 Antibody (MA1-745) in ICC/IF

Immunofluorescence analysis of Syntrophin was done on 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Syntrophin (1351) Mouse Monoclonal Antibody (Product # MA1-745) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28... View More

SNTB2 Antibody in Immunohistochemistry (IHC)

SNTB2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

SNTB2 Antibody — Protein schematic illustrates the common motif and domain organization found in the syntrophin isoforms.

Product Details

MA1-745

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-1 µg/mL

View 17 publications 17 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1-2 µg/mL

View 3 publications 3 publications

Flow Cytometry (Flow)

0.5 µg / 10^6 cells

Immunoprecipitation (IP)

Assay-dependent

View 2 publications 2 publications

Product Specifications

Species Reactivity

Amphibian, Dog, Chicken, Human, Mouse, Rat

Published species

Amphibian, Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1351

Immunogen

Whole purified syntrophin from Torpedo californica electric organ postsynaptic membrane.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2191939

Product Specific Information

MA1-745 detects alpha 1, beta 1 and beta 2 subunits of syntrophin. This antibody has been shown to react with human, mouse, rat, chicken, canine and amphibian samples.

MA1-745 has been successfully used in Western blot, immunoprecipitation and immunofluorescence procedures. By Western blot, this antibody detects an ~58 kDa protein representing syntrophin from rat and mouse cell extracts.

The MA1-745 immunogen is whole purified syntrophin from Torpedo californica electric organ postsynaptic membrane. Characterization studies of this antibody have demonstrated that clone 1351 is directed against an epitope within the PDZ domain of syntrophin. MA1-745, clone 1351, is seen as the "gold standard" for syntrophin assessment.

Target Information

Syntrophins are structurally related proteins located at the neuromuscular synapse and play an important role in dystrophin associated protein (DAP) complex formation. To date there have been three distinct syntrophin isoforms discovered: alpha 1, beta 1, and beta 2. These proteins each contain a PDZ domain, a syntrophin-unique domain (SU), and three pleckstrin homology domains (PH1a,1b and 2). The PDZ domain has been shown to be responsible for binding syntrophin to nNOS and the DAP complex. This association results in localization of the nicotinic acetylcholine receptor to the cytoskeleton via the (S/T)XV C terminus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Metronomic 5-Fluorouracil Delivery Primes Skeletal Muscle for Myopathy but Does Not Cause Cachexia.

Pharmaceuticals (Basel, Switzerland)

Campelj DG,Timpani CA,Cree T,Petersen AC,Hayes A,Goodman CA,Rybalka E

MA1-745 was used in Western Blot to investigate the effects of a chemotherapeutic agent, 5-fluorouracil (5FU), on skeletal muscle mass and function, and whether small-molecule therapeutic candidate, BGP-15, could be protective against the chemotoxic challenge exerted by 5FU.

Mon May 17 00:00:00 UTC 2021

Metronomic 5-Fluorouracil Delivery Primes Skeletal Muscle for Myopathy but Does Not Cause Cachexia.

Pharmaceuticals (Basel, Switzerland)

Campelj DG,Timpani CA,Cree T,Petersen AC,Hayes A,Goodman CA,Rybalka E

Mon May 17 00:00:00 UTC 2021

β1 Syntrophin Supports Autophagy Initiation and Protects against Cerulein-Induced Acute Pancreatitis.

The American journal of pathology

Ye R,Onodera T,Blanchard PG,Kusminski CM,Esser V,Brekken RA,Scherer PE

MA1-745 was used in Western Blotting to reveal, for the first time, that β1 syntrophin is a critical regulator of actin cytoskeleton and autophagy in pancreatic acinar cells and is potently protective against cerulein-induced acute pancreatitis.

Mon Apr 01 00:00:00 UTC 2019

Gain-of-function defects of astrocytic Kir4.1 channels in children with autism spectrum disorders and epilepsy.

Scientific reports

Sicca F,Ambrosini E,Marchese M,Sforna L,Servettini I,Valvo G,Brignone MS,Lanciotti A,Moro F,Grottesi A,Catacuzzeno L,Baldini S,Hasan S,D'Adamo MC,Franciolini F,Molinari P,Santorelli FM,Pessia M

MA1-745 was used in western blot investigate the role of Kir4.1 variants to autism-epilepsy comorbidity

Wed Sep 28 00:00:00 UTC 2016

Neuronal nitric oxide synthase localizes to utrophin expressing intercalated discs and stabilizes their structural integrity.

Neuromuscular disorders : NMD

Gonzalez JP,Crassous PA,Schneider JS,Beuve A,Fraidenraich D

MA1-745 was used in immunohistochemistry - frozen section to study the localization of neuronal nitric oxide synthase to utrophin expressing intercalated discs and stabilization of their structural integrity

Tue Dec 01 00:00:00 UTC 2015

PDZ domain-binding motif regulates cardiomyocyte compartment-specific NaV1.5 channel expression and function.

Circulation

Shy D,Gillet L,Ogrodnik J,Albesa M,Verkerk AO,Wolswinkel R,Rougier JS,Barc J,Essers MC,Syam N,Marsman RF,van Mil AM,Rotman S,Redon R,Bezzina CR,Remme CA,Abriel H

MA1-745 was used in Western Blotting to demonstrate the significance of the PDZ domain-binding motif in the correct expression of NaV1.5, and the role of NaV1.5 in ventricular conduction.

Tue Jul 08 00:00:00 UTC 2014

Genetically induced dysfunctions of Kir2.1 channels: implications for short QT3 syndrome and autism-epilepsy phenotype.

Human molecular genetics

Ambrosini E,Sicca F,Brignone MS,D'Adamo MC,Napolitano C,Servettini I,Moro F,Ruan Y,Guglielmi L,Pieroni S,Servillo G,Lanciotti A,Valvo G,Catacuzzeno L,Franciolini F,Molinari P,Marchese M,Grottesi A,Guerrini R,Santorelli FM,Priori S,Pessia M

MA1-745 was used in western blot to study a naturally occurring Kir2.1 mutation associated with short QT3 syndrome and autism-epilepsy

Mon Sep 15 00:00:00 UTC 2014

Human T-cell leukemia virus type 1 Tax protein interacts with and mislocalizes the PDZ domain protein MAGI-1.

Cancer science

Makokha GN,Takahashi M,Higuchi M,Saito S,Tanaka Y,Fujii M

MA1-745 was used in western blot to study the subcellular mislocalization of MAGI-1 by the Tax oncoprotein of HTLV-1 and the significance for the persistence of HTLV-1 infection

Fri Mar 01 00:00:00 UTC 2013

Phosphorylation on threonine 11 of β-dystrobrevin alters its interaction with kinesin heavy chain.

The FEBS journal

Fratini F,Macchia G,Torreri P,Matteucci A,Salzano AM,Crescenzi M,Macioce P,Petrucci TC,Ceccarini M

MA1-745 was used in western blot to study the role of beta-dystrobrevin threonine 11 phosphorylation in modulating its interaction with the kinesin heavy chain

Thu Nov 01 00:00:00 UTC 2012

Absence of glial α-dystrobrevin causes abnormalities of the blood-brain barrier and progressive brain edema.

The Journal of biological chemistry

Lien CF,Mohanta SK,Frontczak-Baniewicz M,Swinny JD,Zablocka B,Górecki DC

MA1-745 was used in western blot to investigate the role of glial alpha-dystrobrevin in the breakdown of the blood-brain barrier and progressive brain edema

Fri Nov 30 00:00:00 UTC 2012

Knockdown of MLC1 in primary astrocytes causes cell vacuolation: a MLC disease cell model.

Neurobiology of disease

Duarri A,Lopez de Heredia M,Capdevila-Nortes X,Ridder MC,Montolio M,López-Hernández T,Boor I,Lien CF,Hagemann T,Messing A,Gorecki DC,Scheper GC,Martínez A,Nunes V,van der Knaap MS,Estévez R

MA1-745 was used in immunocytochemistry to develop a model of megalencephalic leukoencephalopathy with subcortical cysts (MLC) disease

Fri Jul 01 00:00:00 UTC 2011

The beta1 subunit of the Na,K-ATPase pump interacts with megalencephalic leucoencephalopathy with subcortical cysts protein 1 (MLC1) in brain astrocytes: new insights into MLC pathogenesis.

Human molecular genetics

Brignone MS,Lanciotti A,Macioce P,Macchia G,Gaetani M,Aloisi F,Petrucci TC,Ambrosini E

Published figure using SNTB2 monoclonal antibody (Product # MA1-745) in Western Blot

Sat Jan 01 00:00:00 UTC 2011

Differential requirement for utrophin in the induced pluripotent stem cell correction of muscle versus fat in muscular dystrophy mice.

PloS one

Beck AJ,Vitale JM,Zhao Q,Schneider JS,Chang C,Altaf A,Michaels J,Bhaumik M,Grange R,Fraidenraich D

MA1-745 was used in western blot to evaluate the stem cell therapy for muscular dystrophy mice

Fri Oct 21 00:00:00 UTC 2011

A high throughput screen identifies chemical modulators of the laminin-induced clustering of dystroglycan and aquaporin-4 in primary astrocytes.

PloS one

Noël G,Stevenson S,Moukhles H

MA1-745 was used in western blot to identify chemical modulators for laminin-induced clustering of dystroglycan and aquaporin-4

Mon Mar 07 00:00:00 UTC 2011

MLC1 trafficking and membrane expression in astrocytes: role of caveolin-1 and phosphorylation.

Neurobiology of disease

Lanciotti A,Brignone MS,Camerini S,Serafini B,Macchia G,Raggi C,Molinari P,Crescenzi M,Musumeci M,Sargiacomo M,Aloisi F,Petrucci TC,Ambrosini E

MA1-745 was used in western blot to study the role of caveolin-1 and phosphorylation in modulating the expression and trafficing of the MLC1 membrane protein

Mon Mar 01 00:00:00 UTC 2010

Synaptic localization of neuroligin 2 in the rodent retina: comparative study with the dystroglycan-containing complex.

Journal of neuroscience research

Lui L,Levinson JN,Noël G,Handrigan GR,Richman JM,El-Husseini A,Moukhles H

MA1-745 was used in immunohistochemistry to determine the localization of neuroligin 2 in rodent retinal synapses

Mon Mar 01 00:00:00 UTC 2010

The 8th and 9th tandem spectrin-like repeats of utrophin cooperatively form a functional unit to interact with polarity-regulating kinase PAR-1b.

Biochemical and biophysical research communications

Yamashita K,Suzuki A,Satoh Y,Ide M,Amano Y,Masuda-Hirata M,Hayashi YK,Hamada K,Ogata K,Ohno S

MA1-745 was used in western blot to investigate the mechanism of the interaction between utrophin and kinase PAR-1b

Fri Jan 01 00:00:00 UTC 2010

Dystrophin-associated protein scaffolding in brain requires alpha-dystrobrevin.

Neuroreport

Bragg AD,Das SS,Froehner SC

MA1-745 was used in immunohistochemistry to investigate the role of alpha-dystrobrevin in brain protein scaffolding

Wed Jul 14 00:00:00 UTC 2010

Blastocyst injection of wild type embryonic stem cells induces global corrections in mdx mice.

PloS one

Stillwell E,Vitale J,Zhao Q,Beck A,Schneider J,Khadim F,Elson G,Altaf A,Yehia G,Dong JH,Liu J,Mark W,Bhaumik M,Grange R,Fraidenraich D

MA1-745 was used in immunohistochemistry and western blot to study the rescue of mdx mice by wild type embryonic stem cells

Thu Jul 02 00:00:00 UTC 2009

Blastocyst injection of wild type embryonic stem cells induces global corrections in mdx mice.

PloS one

Stillwell E,Vitale J,Zhao Q,Beck A,Schneider J,Khadim F,Elson G,Altaf A,Yehia G,Dong JH,Liu J,Mark W,Bhaumik M,Grange R,Fraidenraich D

MA1-745 was used in immunohistochemistry and western blot to study the rescue of mdx mice by wild type embryonic stem cells

Thu Jul 02 00:00:00 UTC 2009

Cardiac sodium channel Nav1.5 is regulated by a multiprotein complex composed of syntrophins and dystrophin.

Circulation research

Gavillet B,Rougier JS,Domenighetti AA,Behar R,Boixel C,Ruchat P,Lehr HA,Pedrazzini T,Abriel H

MA1-745 was used in western blot to investigate the role of syntrophins and dystrophin on the proper expression and function of Na (v) 1.5

Fri Aug 18 00:00:00 UTC 2006

Syntrophins regulate alpha1D-adrenergic receptors through a PDZ domain-mediated interaction.

The Journal of biological chemistry

Chen Z,Hague C,Hall RA,Minneman KP

MA1-745 was used in immunoprecipitation to study the mechanism for syntrophin regulation of alpha1D-adrenergic receptors .

Fri May 05 00:00:00 UTC 2006

Syntrophins regulate alpha1D-adrenergic receptors through a PDZ domain-mediated interaction.

The Journal of biological chemistry

Chen Z,Hague C,Hall RA,Minneman KP

MA1-745 was used in immunoprecipitation to study the mechanism for syntrophin regulation of alpha1D-adrenergic receptors .

Fri May 05 00:00:00 UTC 2006

Dephosphorylation of beta2-syntrophin and Ca2+/mu-calpain-mediated cleavage of ICA512 upon stimulation of insulin secretion.

The EMBO journal

Ort T,Voronov S,Guo J,Zawalich K,Froehner SC,Zawalich W,Solimena M

Published figure using SNTB2 monoclonal antibody (Product # MA1-745) in Western Blot

Wed Aug 01 00:00:00 UTC 2001

Absence of alpha-syntrophin leads to structurally aberrant neuromuscular synapses deficient in utrophin.

The Journal of cell biology

Adams ME,Kramarcy N,Krall SP,Rossi SG,Rotundo RL,Sealock R,Froehner SC

MA1-745 was used in immunoprecipitation to investigate the impacts of the lack of alpha-syntrophin on neuromuscular synapses

Mon Sep 18 00:00:00 UTC 2000

A role of tyrosine phosphatase in acetylcholine receptor cluster dispersal and formation.

The Journal of cell biology

Dai Z,Peng HB

MA1-745 was used in immunocytochemistry to investigate the regulation of AChR cluster dispersal and formation by PTPase

Mon Jun 29 00:00:00 UTC 1998

Bioinformatics

Protein Aliases: 59 kDa dystrophin-associated protein A1 basic component 2; Beta-2-syntrophin; dystrophin-associated protein A1, 59kD, basic component 2; SNT3; SNTL; SYN; syntrophin, basic 2; syntrophin, beta 2 (dystrophin-associated protein A1, 59kDa, basic component 2); Syntrophin-3; Syntrophin-like

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Gene Aliases: D16S2531E; EST25263; Snt2; SNT2B2; SNT3; SNTB2; SNTL

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UniProt ID: (Human) Q13425, (Mouse) Q61235

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Entrez Gene ID: (Human) 6645, (Dog) 479675, (Mouse) 20650, (Rat) 689421

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Function(s)

actin binding protein binding calmodulin binding poly(A) RNA binding structural molecule activity

Performance Guarantee

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SNTB2 Monoclonal Antibody (1351)

SNTB2 Antibody (MA1-745) in ICC/IF

Product Details

MA1-745

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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