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Invitrogen
SOCS3 Polyclonal Antibody
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SOCS3 Antibody (PA5-95044) in IHC (P)
Immunohistochemistry analysis of SOCS3 in paraffin-embedded human tonsil tissues. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with SOCS3 polyclonal antibody (Product # PA5-95044) at a 1 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C), and developed with Strepavidin-Biotin-Complex and DA... View More
Product Details
PA5-95044
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human SOCS3 (71-85aa RDSSDQRHFFTLSVK).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide, 0.05mg thimerosal
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2806850
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human A549 whole cell, human A431 whole cell. IHC: human tonsil tissue.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Accumulating evidence demonstrates that cytokine receptor signaling is negatively regulated by a family of Src homology 2 domain-containing adaptor molecules Termed SOCS (Suppressor of Cytokine Signaling). To date, there are eight members of SOCS family have been recognized, they are SOCS-1, 2, 3, 4, 5, 6, 7 and CIS. Structurally the SOCS proteins are composed of an N-terminal region of variable length and amino acid composition, a central SH2 domain, and a previously unrecognized C-terminal motif that we have called the SOCS box. The SOCS proteins appear to form part of a classical negative feed back loop that regulates cytokine signal transduction via a STAT-induced transcriptional mechanism. Transcription of each of the SOCS genes occurs rapidly in vitro and in vivo in response to cytokines, and once produced, the various members of the SOCS family appear to inhibit signaling in different ways.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CIS-3; cytokine inducible SH2-containing protein 3; Cytokine-inducible SH2 protein 3; E2a-Pbx1 target gene in fibroblasts 10; MGC71791; Protein EF-10; SOCS-3; SSI-3; STAT-induced STAT inhibitor 3; Suppressor of cytokine signaling 3
Gene Aliases: ATOD4; CIS3; Cish3; EF-10; Ef10; SOCS-3; SOCS3; SSI-3; SSI3
UniProt ID: (Human) O14543, (Mouse) O35718
Entrez Gene ID: (Human) 9021, (Mouse) 12702
regulation of protein phosphorylation
negative regulation of protein kinase activity
signal transduction
JAK-STAT cascade
negative regulation of signal transduction
cytokine-mediated signaling pathway
intracellular signal transduction
regulation of growth
negative regulation of apoptotic process
regulation of cell differentiation
positive regulation of cell differentiation
negative regulation of JAK-STAT cascade
negative regulation of insulin receptor signaling pathway
negative regulation of inflammatory response
branching involved in labyrinthine layer morphogenesis
placenta blood vessel development
trophoblast giant cell differentiation
spongiotrophoblast differentiation
response to hypoxia
aging
response to heat
response to gamma radiation
protein ubiquitination
organ regeneration
response to food
response to estradiol
response to lipopolysaccharide
response to progesterone
response to insulin
response to drug
negative regulation of tyrosine phosphorylation of Stat1 protein
positive regulation of tyrosine phosphorylation of Stat3 protein
negative regulation of tyrosine phosphorylation of Stat3 protein
response to glucocorticoid
regulation of interferon-gamma-mediated signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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