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Invitrogen
SOD2 Recombinant Rabbit Monoclonal Antibody (001)
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SOD2 Antibody (MA5-29577) in WB
Western blot was performed using Anti-SOD2 Recombinant Rabbit Monoclonal Antibody (1) (Product # MA5-29577) and ~20 kDa band corresponding to SOD2 was observed in all cell lines, tissues tested, induced after TNFα treatment in A549 cells and Hydrogen peroxide treatment in HeLa, PC-12 cells. Whole cell extracts (40 µg lysate) of A549 (Lane 1), A549 treated with TNFα (20 ng/mL for 72 hrs) (Lane 2), HeLa (Lane 3) HeLa treated with Hydrogen peroxide (50 µM for 6 hrs) (Lane 4), Hep G2 (Lane 5), PC-12 (Lane 6), PC-12 treated with Hydrogen peroxi... View More
Product Details
MA5-29577
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
001
Immunogen
Recombinant Human SOD2 protein (Lys25-Lys222)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2785419
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Human SOD2.
Target Information
Superoxide dismutase (SOD) is an antioxidant enzyme involved in the defense system against reactive oxygen species (ROS). SOD catalyzes the dismutation reaction of superoxide radical anion (O2) to hydrogen peroxide, which is then catalyzed to innocuous O2 and H2O by glutathione peroxidase and catalase. Several classes of SOD have been identified. These include intracellular copper, zinc SOD (Cu, Zn-SOD/SOD-1), mitochondrial manganese SOD (Mn-SOD/SOD-2) and extracellular Cu, Zn-SOD (EC-SOD/SOD-3). SOD1 is found in all eukaryotic species as a homodimeric 32 kDa enzyme containing one each of Cu and Zn ion per subunit. The manganese containing 80 kDa tetrameric enzyme SOD2, is located in the mitochondrial matrix in close proximity to a primary endogenous source of superoxide, the mitochondrial respiratory chain. SOD3 is a heparin-binding multimer of disulfide-linked dimers, primarily expressed in human lungs, vessel walls and airways. SOD4 is a copper chaperone for superoxide dismutase (CCS), which specifically delivers Cu to copper/zinc superoxide dismutase. CCS may activate copper/zinc superoxide dismutase through direct insertion of the Cu cofactor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: indophenoloxidase B; IPO B; manganese-containing superoxide dismutase; mangano-superoxide dismutase; Mn superoxide dismutase; superoxide dismutase; Superoxide dismutase [Mn], mitochondrial
Gene Aliases: IPO-B; IPOB; Mn-SOD; MNSOD; MVCD6; SOD2
UniProt ID: (Human) P04179
Entrez Gene ID: (Human) 6648
response to reactive oxygen species
response to superoxide
age-dependent response to reactive oxygen species
release of cytochrome c from mitochondria
vasodilation by acetylcholine involved in regulation of systemic arterial blood pressure
regulation of transcription from RNA polymerase II promoter
superoxide metabolic process
regulation of blood pressure
negative regulation of cell proliferation
removal of superoxide radicals
oxygen homeostasis
negative regulation of neuron apoptotic process
protein homotetramerization
oxidation-reduction process
negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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