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OriGene
SOD2 Monoclonal Antibody (OTI1H6), TrueMAB™
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FIGURE: 1 / 2
SOD2 Antibody (CF501887) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SOD2 (RC202330, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-SOD2. Positive lysates LY400235 (100 µg) and LC400235 (20 µg) can be purchased separately from OriGene.
Product Details
CF501887
Product Specifications
Species Reactivity
Dog,
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI1H6
Immunogen
Full length human recombinant protein of human SOD2 produced in HEK293T cell.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
Superoxide dismutase (SOD) is an antioxidant enzyme involved in the defense system against reactive oxygen species (ROS). SOD catalyzes the dismutation reaction of superoxide radical anion (O2) to hydrogen peroxide, which is then catalyzed to innocuous O2 and H2O by glutathione peroxidase and catalase. Several classes of SOD have been identified. These include intracellular copper, zinc SOD (Cu, Zn-SOD/SOD-1), mitochondrial manganese SOD (Mn-SOD/SOD-2) and extracellular Cu, Zn-SOD (EC-SOD/SOD-3). SOD1 is found in all eukaryotic species as a homodimeric 32 kDa enzyme containing one each of Cu and Zn ion per subunit. The manganese containing 80 kDa tetrameric enzyme SOD2, is located in the mitochondrial matrix in close proximity to a primary endogenous source of superoxide, the mitochondrial respiratory chain. SOD3 is a heparin-binding multimer of disulfide-linked dimers, primarily expressed in human lungs, vessel walls and airways. SOD4 is a copper chaperone for superoxide dismutase (CCS), which specifically delivers Cu to copper/zinc superoxide dismutase. CCS may activate copper/zinc superoxide dismutase through direct insertion of the Cu cofactor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: indophenoloxidase B; IPO B; manganese-containing superoxide dismutase; mangano-superoxide dismutase; Mn superoxide dismutase; superoxide dismutase; Superoxide dismutase [Mn], mitochondrial
Gene Aliases: IPO-B; IPOB; Mn-SOD; MNSOD; MVCD6; SOD2
UniProt ID: (Human) P04179
Entrez Gene ID: (Human) 6648, (Dog) 476258
response to reactive oxygen species
response to superoxide
age-dependent response to reactive oxygen species
release of cytochrome c from mitochondria
vasodilation by acetylcholine involved in regulation of systemic arterial blood pressure
regulation of transcription from RNA polymerase II promoter
superoxide metabolic process
regulation of blood pressure
negative regulation of cell proliferation
removal of superoxide radicals
oxygen homeostasis
negative regulation of neuron apoptotic process
protein homotetramerization
oxidation-reduction process
negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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