Proteintech

SOX2 Polyclonal Antibody

SOX2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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SOX2 Antibody (20118-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded mouse embryo tissue slide using 20118-1-AP ( SOX2 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

SOX2 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

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Product Details

20118-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 13 publications 13 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 3 publications 3 publications

Flow Cytometry (Flow)

Assay-dependent

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SOX2 Fusion Protein Ag13635 (1-317 aa encoded by BC013923)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.33 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody is a rabbit polyclonal antibody raised against an internal region of human SOX2. a rare undifferentiated cell population that is intermingled with the Bergmann glia of the adult murine cerebellar cortex, expresses the stem cell markers Sox2 and Nestin, and lacks markers of glial or neuronal differentiation. Sox2-expressing neural stem cells in the subgranular zone (SGZ), a well-known stem cell niche of the adult brain.

Immunogen sequence: MYNMMETEL KPPGPQQTSG GGGGNSTAAA AGGNQKNSPD RVKRPMNAFM VWSRGQRRKM AQENPKMHNS EISKRLGAEW KLLSETEKRP FIDEAKRLRA LHMKEHPDYK YRPRRKTKTL MKKDKYTLPG GLLAPGGNSM ASGVGVGAGL GAGVNQRMDS YAHMNGWSNG SYSMMQDQLG YPQHPGLNAH GAAQMQPMHR YDVSALQYNS MTSSQTYMNG SPTYSMSYSQ QGTPGMALGS MGSVVKSEAS SSPPVVTSSS HSRAPCQAGD LRDMISMYLP GAEVPEPAAP SRLHMSQHYQ SGPVPGTAIN GTLPLSHM (1-317 aa encoded by BC013923)

Target Information

SOX2 is an intronless gene encoding a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of the SOX2 gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. The SOX2 gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). Further, SOX2 protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation, optic nerve hypoplasia and syndromic microphthalmia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Extracellular HSPA5 is autocrinally involved in the regulation of neuronal process elongation.

Biochemical and biophysical research communications

Fukawa M,Shirai R,Torii T,Nakata K,Fukatsu S,Sato T,Homma K,Miyamoto Y,Yamauchi J

Wed Jul 05 00:00:00 UTC 2023

KK-LC-1 as a therapeutic target to eliminate ALDH⁺ stem cells in triple negative breast cancer.

Nature communications

Bu J,Zhang Y,Wu S,Li H,Sun L,Liu Y,Zhu X,Qiao X,Ma Q,Liu C,Niu N,Xue J,Chen G,Yang Y,Liu C

Fri May 05 00:00:00 UTC 2023

KK-LC-1 as a therapeutic target to eliminate ALDH⁺ stem cells in triple negative breast cancer.

Nature communications

Bu J,Zhang Y,Wu S,Li H,Sun L,Liu Y,Zhu X,Qiao X,Ma Q,Liu C,Niu N,Xue J,Chen G,Yang Y,Liu C

Fri May 05 00:00:00 UTC 2023

Patients' Stem Cells Differentiation in a 3D Environment as a Promising Experimental Tool for the Study of Amyotrophic Lateral Sclerosis.

International journal of molecular sciences

Scarian E,Bordoni M,Fantini V,Jacchetti E,Raimondi MT,Diamanti L,Carelli S,Cereda C,Pansarasa O

Wed May 11 00:00:00 UTC 2022

CHD4 mediates SOX2 transcription through TRPS1 in luminal breast cancer.

Cellular signalling

Zhang J,Lv X,Wei B,Gong X,Chen L

Thu Dec 01 00:00:00 UTC 2022

Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM).

Nature methods

Laporte MH,Klena N,Hamel V,Guichard P

Tue Feb 01 00:00:00 UTC 2022

p57 Suppresses the Pluripotency and Proliferation of Mouse Embryonic Stem Cells by Positively Regulating p53 Activation.

Stem cells international

Li N,Du Z,Li Y,Xu W,Yang Y,Peng H,Song T,Qin Q,Lei H,Hua J

Tue Jan 04 00:00:00 UTC 2022

Effect of the interference with DRP1 expression on the biological characteristics of glioma stem cells.

Experimental and therapeutic medicine

Zhang L,Cao H,Tao H,Yang J,Gong W,Hu Q

Thu Jul 01 00:00:00 UTC 2021

HET0016 attenuates the stemness of breast cancer cells through targeting CYP4Z1.

Molecular carcinogenesis

Liu H,Qin H,Zhou Y,Yuan Y,Liu Y,Chen Y,Yang Y,Ni H,Xi T,Zheng L

Tue Jun 01 00:00:00 UTC 2021

LncRNA SOX2OT affects cervical cancer cell growth, migration and invasion by regulating SOX2.

Cell cycle (Georgetown, Tex.)

Chang X,Zhang H,Yang Q,Pang L

Mon Jun 01 00:00:00 UTC 2020

Acetyl-11-keto-β-boswellic acid suppresses docetaxel-resistant prostate cancer cells in vitro and in vivo by blocking Akt and Stat3 signaling, thus suppressing chemoresistant stem cell-like properties.

Acta pharmacologica Sinica

Liu YQ,Wang SK,Xu QQ,Yuan HQ,Guo YX,Wang Q,Kong F,Lin ZM,Sun DQ,Wang RM,Lou HX

Wed May 01 00:00:00 UTC 2019

Acta pharmacologica Sinica

Liu YQ,Wang SK,Xu QQ,Yuan HQ,Guo YX,Wang Q,Kong F,Lin ZM,Sun DQ,Wang RM,Lou HX

Wed May 01 00:00:00 UTC 2019

Early expression of Tubulin Beta-III in avian cranial neural crest cells.

Gene expression patterns : GEP

Chacon J,Rogers CD

Sun Dec 01 00:00:00 UTC 2019

Knockdown of SALL4 expression using RNA interference induces cell cycle arrest, enhances early apoptosis, inhibits invasion and increases chemosensitivity to temozolomide in U251 glioma cells.

Oncology letters

Zhang L,Yan Y,Jiang Y,Qian J,Jiang L,Hu G,Lu Y,Luo C

Sun Oct 01 00:00:00 UTC 2017

Knockdown of SALL4 expression using RNA interference induces cell cycle arrest, enhances early apoptosis, inhibits invasion and increases chemosensitivity to temozolomide in U251 glioma cells.

Oncology letters

Zhang L,Yan Y,Jiang Y,Qian J,Jiang L,Hu G,Lu Y,Luo C

Sun Oct 01 00:00:00 UTC 2017

Linc-DYNC2H1-4 promotes EMT and CSC phenotypes by acting as a sponge of miR-145 in pancreatic cancer cells.

Cell death & disease

Gao Y,Zhang Z,Li K,Gong L,Yang Q,Huang X,Hong C,Ding M,Yang H

Thu Jul 13 00:00:00 UTC 2017

Linc-DYNC2H1-4 promotes EMT and CSC phenotypes by acting as a sponge of miR-145 in pancreatic cancer cells.

Cell death & disease

Gao Y,Zhang Z,Li K,Gong L,Yang Q,Huang X,Hong C,Ding M,Yang H

Thu Jul 13 00:00:00 UTC 2017

Aldehyde dehydrogenase 1A1 up-regulates stem cell markers in benzo[a]pyrene-induced malignant transformation of BEAS-2B cells.

Environmental toxicology and pharmacology

Liu Y,Lu R,Gu J,Chen Y,Zhang X,Zhang L,Wu H,Hua W,Zeng J

Fri Jul 01 00:00:00 UTC 2016

Aldehyde dehydrogenase 1A1 up-regulates stem cell markers in benzo[a]pyrene-induced malignant transformation of BEAS-2B cells.

Environmental toxicology and pharmacology

Liu Y,Lu R,Gu J,Chen Y,Zhang X,Zhang L,Wu H,Hua W,Zeng J

Fri Jul 01 00:00:00 UTC 2016

Knocking down of heat-shock protein 27 directs differentiation of functional glutamatergic neurons from placenta-derived multipotent cells.

Scientific reports

Cheng YC,Huang CJ,Lee YJ,Tien LT,Ku WC,Chien R,Lee FK,Chien CC

Fri Jul 22 00:00:00 UTC 2016

Small-Activating RNA Can Change Nucleosome Positioning in Human Fibroblasts.

Journal of biomolecular screening

Wang B,Sun J,Shi J,Guo Q,Tong X,Zhang J,Hu N,Hu Y

Fri Jul 01 00:00:00 UTC 2016

Small-Activating RNA Can Change Nucleosome Positioning in Human Fibroblasts.

Journal of biomolecular screening

Wang B,Sun J,Shi J,Guo Q,Tong X,Zhang J,Hu N,Hu Y

Fri Jul 01 00:00:00 UTC 2016

Epigenetic reprogramming of human lung cancer cells with the extract of bovine parthenogenetic oocytes.

Journal of cellular and molecular medicine

Wang Z,Dao R,Bao L,Dong Y,Wang H,Han P,Yue Y,Yu H

Mon Sep 01 00:00:00 UTC 2014

Epigenetic reprogramming of human lung cancer cells with the extract of bovine parthenogenetic oocytes.

Journal of cellular and molecular medicine

Wang Z,Dao R,Bao L,Dong Y,Wang H,Han P,Yue Y,Yu H

Mon Sep 01 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: Delta EF2a; MCOPS3 (Microphthalmia Syndromic type 3); sex determining region Y-box 2; SOX 2; SRY (sex determining region Y) box 2; SRY (sex determining region Y)-box 2; SRY box 2; SRY related HMG box 2; SRY-box containing gene 2; SRY-related HMG-box gene 2; Transcription factor SOX-2; transcription factor SOX2

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Gene Aliases: ANOP3; lcc; MCOPS3; RGD1565646; Sox-2; SOX2; ysb

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UniProt ID: (Human) P48431, (Mouse) P48432

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Entrez Gene ID: (Human) 6657, (Rat) 499593, (Mouse) 20674

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Function(s)

transcription regulatory region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding miRNA binding sequence-specific DNA binding transcription regulatory region DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding molecular_function chromatin binding transcription factor binding protein heterodimerization activity chromatin DNA binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter osteoblast differentiation eye development endodermal cell fate specification chromatin organization regulation of transcription, DNA-templated transcription from RNA polymerase II promoter cell cycle arrest response to wounding regulation of gene expression glial cell fate commitment pituitary gland development adenohypophysis development positive regulation of cell-cell adhesion forebrain development somatic stem cell population maintenance tissue regeneration regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of MAPK cascade positive regulation of cell differentiation negative regulation of neuron differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter inner ear development negative regulation of epithelial cell proliferation response to growth factor negative regulation of canonical Wnt signaling pathway neuronal stem cell population maintenance cell fate specification positive regulation of neuroblast proliferation regulation of transcription from RNA polymerase II promoter sensory perception of sound response to organic substance stem cell population maintenance forebrain neuron differentiation cerebral cortex development negative regulation of Wnt signaling pathway male genitalia development positive regulation of epithelial cell differentiation olfactory placode formation response to retinoic acid inner ear morphogenesis tongue development cell fate commitment negative regulation of cell differentiation positive regulation of neuron differentiation negative regulation of osteoblast differentiation positive regulation of Notch signaling pathway pigment biosynthetic process lung alveolus development embryonic organ development anatomical structure formation involved in morphogenesis neuron fate commitment diencephalon morphogenesis stem cell differentiation regulation of neurogenesis detection of mechanical stimulus involved in sensory perception of sound detection of mechanical stimulus involved in equilibrioception retina morphogenesis in camera-type eye lens induction in camera-type eye epithelial tube branching involved in lung morphogenesis transcription, DNA-templated multicellular organism development

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SOX2 Polyclonal Antibody

SOX2 Antibody (20118-1-AP) in IHC (P)

Product Details

20118-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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