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SOX5 Antibody (MA5-55559) in WB
Western blot analysis of lysates from Mouse testis, using SOX5 Recombinant Monoclonal Antibody (Product # MA5-55559) at 1:2,000 dilution followed by a HRP-conjugated Goat anti-Rabbit IgG (H+L) secondary antibody at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Product Details
MA5-55559
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
7W1J1
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SOX5.
Conjugate
Form
Liquid
Concentration
1.35 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
RRID
AB_3667798
Product Specific Information
Immunogen Sequence: MLTDPDLPQE FERMSSKRPA SPYGEADGEV AMVTSRQKVE EEESDGLPAF HLPLHVSFPN KPHSEEFQPV SLLTQETCGH RTPTSQHNTM EVDGNKVMSS
Target Information
This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. The encoded protein may play a role in chondrogenesis. A pseudogene of this gene is located on chromosome 8. Multiple transcript variants encoding distinct isoforms have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MGC35153; Sox5-BLM isoform; SRY-box containing gene 5; SRY-box containing gene 5-like 1; Transcription factor SOX-5
Gene Aliases: A730017D01Rik; AI528773; Sox-5; Sox5; Sox5l1
UniProt ID: (Rat) F1M8W4, (Mouse) P35710
Entrez Gene ID: (Rat) 140587, (Mouse) 20678
in utero embryonic development
transcription from RNA polymerase II promoter
central nervous system neuron differentiation
positive regulation of chondrocyte differentiation
cell fate commitment
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
oligodendrocyte differentiation
cartilage development
regulation of timing of neuron differentiation
positive regulation of cartilage development
cellular response to transforming growth factor beta stimulus
positive regulation of mesenchymal stem cell differentiation
transcription, DNA-templated
regulation of transcription, DNA-templated
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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