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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • SOX9 Antibodies

          Invitrogen

          SOX9 Monoclonal Antibody (GMPR9), eBioscience™

          Advanced Verification
          3 References
          View all (54) SOX9 antibodies

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          Datasheet
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          Datasheet
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          Cite SOX9 Monoclonal Antibody (GMPR9), eBioscience™

          • Antibody Testing Data (7)
          • Advanced Verification (3)
          SOX9 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          SOX9 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 10

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          SOX9 Antibody (14-9765-82) in ICC/IF

          Immunocytochemistry of fixed and permeabilized HepG2 cells using 1 µg/mL of Anti-Human/Mouse Sox9 Purified followed by 1 µg/mL of F (ab')2 Anti-Mouse IgG eFluor® 570.Nuclei are counterstained with DAPI, and colocalization of signal appears pink (left).Immunohistochemistry of formalin-fixed paraffin embedded human tonsil using 1 µg/mL of Anti-Human/Mouse Sox9 Purified followed by Anti-Mouse IgG Biotin, Streptavidin HRP, and DAB visualization.Nuclei are counterstained with hematoxylin (right). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          SOX9 Antibody in Immunocytochemistry (ICC/IF)
          SOX9 Antibody in Immunocytochemistry (ICC/IF)
          SOX9 Antibody in Western Blot (WB)
          SOX9 Antibody in Western Blot (WB)
          SOX9 Antibody in Western Blot (WB)
          SOX9 Antibody in ChIP Assay (ChIP)
          SOX9 Antibody in ChIP Assay (ChIP)
          SOX9 Antibody
          SOX9 Antibody
          SOX9 Antibody

          Product Details

          14-9765-82

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.5 µg/mL
          -

          Immunohistochemistry (IHC)

          -
          View 2 publications 2 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1 µg/mL
          -

          Immunocytochemistry (ICC/IF)

          1 µg/mL
          View 1 publication 1 publication

          ChIP assay (ChIP)

          2.5 µg/10^6 cells
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Human, Mouse

          Host/Isotype

          Mouse / IgG1, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          GMPR9

          Conjugate

          Unconjugated Unconjugated Unconjugated
          • Alexa Fluor 488 
          • Request custom conjugation

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          0.09% sodium azide

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2573006

          Product Specific Information

          Description: This GMPR9 monoclonal antibody reacts with human and mouse Sox9, a member of the Sry HMG-box (Sox) gene family of transcription factors. Like Sry, Sox9 contains a high mobility group (HMG-box) DNA-binding domain. The Sox9 DNA-binding domain recognizes the sequence AGAACAATGG, and when bound to co-factors, functions to bend the DNA. This structural change is thought to be important for chromatin remodeling and the initiation of more broad transcriptional regulation of multiple genes. Sox9 is expressed in multiple tissue types during development and its activation of downstream targets is determined by interaction with tissue-specific co-factors. During development, Sox9 expression is important for cartilage formation (mesenchymal to chondrocyte), sex determination (testes formation), neural crest formation and retinal development. Sox9 plays a role in the differentiation of multiple cell types including glial, hair, cardiac, melanocytes, pancreatic, prostate, and intestinal epithelium. Sox9 regulates expression of the following family member genes sox5, sox6, and sox10, in addition to collagen type 2 alpha, collagen 9A1, CEACAM1, and snail2. Sox9 dysregulation is involved in craniofacial defects, cleft palate, sex reversal, and some types of cancer (melanoma, lung adenocarcinoma, and breast cancer). Sox9 has also been shown to be expressed in adult stem cells in the liver, pancreas and intestine.

          Applications Reported: This GMPR9 antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry.

          Applications Tested: This GMPR9 antibody has been tested by immunohistochemistry of formalin fixed paraffin embedded human tissue using high or low pH antigen retrieval and can be used at less than or equal to 1 µg/mL. This GMPR9 antibody has been tested by immunocytochemistry of fixed and permeabilized human cells and can be used at less than or equal to 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Purity: Greater than 90%, as determined by SDS-PAGE.

          Aggregation: Less than 10%, as determined by HPLC.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          SOX9 has a role in sex determination and differentiation of Sertoli cells. It is involved in chondrogenesis and regulates the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes. Translocation of this gene can cause campomelic dysplasia. SOX9 is involved in the formation of testes from the indifferent fetal gonads. It is a major molecular component of the neuron-glia switch in developing spinal cord. During normal development, SOX9 allows the prostate epithelium to outgrow into the mesenchyme and then provides basal cell support for development and maintenance of the luminal epithelium. These functions of SOX9 are subverted in prostate cancer to support tumor growth and invasion. SOX9 may direct the formation of neural crest precursors and the development of a range of neural crest derivative. It has a transcriptional regulation in melanin production in cells. Northern blot analysis shows its expression on adult testis, adult heart, and fetal brain.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: mSox9; SOX 9; SRY (sex determining region Y)-box 9; SRY (sex determining region Y)-box 9 (campomelic dysplasia, autosomal sex-reversal); SRY (sex determining region Y)-box9; SRY (sex-determining region Y)-box 9 protein; SRY box 9; SRY-Box 9 ; SRY-box containing gene 9; SRY-related HMG-box, gene 9; Transcription factor SOX-9

          View more View less

          Gene Aliases: 2010306G03Rik; AV220920; CMD1; CMPD1; mKIAA4243; Sox-9; SOX9; SRA1; SRXX2; SRXY10

          View more View less

          UniProt ID: (Human) P48436, (Rat) F1LYL9, (Mouse) Q04887

          View more View less

          Entrez Gene ID: (Human) 6662, (Rat) 140586, (Mouse) 20682

          View more View less

          Function(s)
          core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding enhancer sequence-specific DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding protein kinase activity protein binding beta-catenin binding protein kinase A catalytic subunit binding enhancer binding bHLH transcription factor binding protein heterodimerization activity pre-mRNA intronic binding transcription regulatory region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding sequence-specific DNA binding transcription regulatory region DNA binding
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter skeletal system development cartilage condensation ossification branching involved in ureteric bud morphogenesis cell fate specification epithelial to mesenchymal transition tissue homeostasis positive regulation of protein phosphorylation hair follicle development positive regulation of mesenchymal cell proliferation chondrocyte development negative regulation of immune system process heart valve development heart valve morphogenesis heart valve formation endocardial cushion morphogenesis chondrocyte differentiation involved in endochondral bone morphogenesis chondrocyte hypertrophy nucleosome assembly chromatin remodeling transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter protein complex assembly protein phosphorylation cytoskeleton organization signal transduction epidermal growth factor receptor signaling pathway spermatogenesis heart development positive regulation of cell proliferation negative regulation of cell proliferation male gonad development regulation of cell cycle process positive regulation of gene expression negative regulation of gene expression positive regulation of epithelial cell migration neural crest cell development positive regulation of phosphatidylinositol 3-kinase signaling single organismal cell-cell adhesion male germ-line sex determination cAMP-mediated signaling cell differentiation regulation of cell adhesion extracellular matrix organization male sex determination negative regulation of ossification negative regulation of bone mineralization prostate gland development negative regulation of epithelial cell differentiation positive regulation of epithelial cell differentiation mammary gland development notochord development otic vesicle formation endocrine pancreas development negative regulation of chondrocyte differentiation positive regulation of chondrocyte differentiation lacrimal gland development protein localization to nucleus somatic stem cell population maintenance intrahepatic bile duct development regulation of cell proliferation regulation of apoptotic process negative regulation of apoptotic process protein kinase B signaling cell fate commitment regulation of cell differentiation negative regulation of myoblast differentiation positive regulation of protein catabolic process negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of photoreceptor cell differentiation oligodendrocyte differentiation homeostasis of number of cells within a tissue positive regulation of epithelial cell proliferation negative regulation of epithelial cell proliferation cartilage development Sertoli cell differentiation Sertoli cell development astrocyte fate commitment retina development in camera-type eye limb bud formation retinal rod cell differentiation endochondral bone morphogenesis epithelial tube branching involved in lung morphogenesis lung epithelial cell differentiation prostate gland morphogenesis epithelial cell proliferation involved in prostatic bud elongation bronchus cartilage development trachea cartilage development intestinal epithelial structure maintenance regulation of cell proliferation involved in tissue homeostasis positive regulation of cartilage development regulation of branching involved in lung morphogenesis morphogenesis of a branching epithelium lung smooth muscle development negative regulation of biomineral tissue development ERK1 and ERK2 cascade Harderian gland development cellular response to mechanical stimulus cellular response to retinoic acid cellular response to interleukin-1 cellular response to epidermal growth factor stimulus cellular response to heparin cellular response to transforming growth factor beta stimulus renal vesicle induction ureter development ureter urothelium development ureter smooth muscle cell differentiation ureter morphogenesis metanephric nephron tubule formation negative regulation of canonical Wnt signaling pathway cochlea morphogenesis positive regulation of kidney development positive regulation of branching involved in ureteric bud morphogenesis positive regulation of extracellular matrix assembly positive regulation of male gonad development positive regulation of cell proliferation involved in heart morphogenesis positive regulation of mesenchymal stem cell differentiation regulation of epithelial cell proliferation involved in lung morphogenesis negative regulation of mesenchymal cell apoptotic process Notch signaling pathway
          It has to be done as per old AB suggested Products section.
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