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          • Primary Antibodies ›
          • SPAG16 Antibodies

          Proteintech

          SPAG16 Polyclonal Antibody

          1 Reference
          View all (9) SPAG16 antibodies

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          Datasheet
          Protocols
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          Datasheet
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          Cite SPAG16 Polyclonal Antibody

          • Antibody Testing Data (6)
          SPAG16 Antibody in Immunocytochemistry (ICC/IF)
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          SPAG16 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 6

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          SPAG16 Antibody (16883-1-AP) in ICC/IF

          Immunofluorescent analysis of HepG2 cells, using SPAG16 antibody 16883-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          SPAG16 Antibody in Immunocytochemistry (ICC/IF)
          SPAG16 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SPAG16 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SPAG16 Antibody in Western Blot (WB)
          SPAG16 Antibody in Western Blot (WB)
          SPAG16 Antibody in Immunoprecipitation (IP)

          Product Details

          16883-1-AP

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          View 1 publication 1 publication

          Immunohistochemistry (Paraffin) (IHC (P))

          1:20-1:200
          -

          Immunocytochemistry (ICC/IF)

          1:20-1:200
          -

          Immunoprecipitation (IP)

          0.5-4.0 µg
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          SPAG16 Fusion Protein Ag10348 (1-181 aa encoded by BC009614)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.14 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice (domestic); Dry ice (international)

          Product Specific Information

          This antibody was raised against the N-terminal region of SPAG16 and mainly recognizes SPAG16L.

          Immunogen sequence: MAAQRGMPS SAVRVLEEAL GMGLTAAGDA RDTADAVAAE GAYYLEQVTI TEASEDDYEY EEIPDDNFSI PEGEEDLAKA IQMAQEQATD TEILERKTVL PSKHAVPEVI EDFLCNFLIK MGMTRTLDCF QSEWYELIQK GVTELRTVGN VPDVYTQIML LENENKNLKK DLKHYKQAAE YV (1-181 aa encoded by BC009614)

          Target Information

          Cilia and flagella are comprised of a microtubular backbone, the axoneme, which is organized by the basal body and surrounded by plasma membrane. SPAG16 encodes 2 major proteins that associate with the axoneme of sperm tail and the nucleus of postmeiotic germ cells, respectively.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Pf20 protein homolog; Sperm-associated antigen 16 protein; sperm-associated WD repeat protein; spg16; WD repeat domain 29

          View more View less

          Gene Aliases: 4921511D23Rik; 4930524F24Rik; 4930585K05Rik; AV261009; PF20; RGD1565062; SPAG16; WDR29

          View more View less

          UniProt ID: (Human) Q8N0X2, (Mouse) Q8K450

          View more View less

          Entrez Gene ID: (Human) 79582, (Mouse) 66722, (Rat) 501158

          View more View less

          Function(s)
          protein kinase binding protein binding
          Process(es)
          sperm axoneme assembly cell projection organization axoneme assembly cilium assembly microtubule sliding cilium movement involved in cell motility cell motility in response to calcium ion spermatogenesis
          It has to be done as per old AB suggested Products section.
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