Invitrogen

SPARC Polyclonal Antibody

SPARC Antibody in Immunocytochemistry (ICC/IF)

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SPARC Antibody (PA5-78178) in ICC/IF

Immunofluorescence analysis of SPARC was performed using 70% confluent log phase BJ cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with SPARC Polyclonal Antibody (Product # PA5-78178) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790... View More

SPARC Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SPARC Antibody in Western Blot, Immunohistochemistry (WB, IHC)

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SPARC Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of SPARC was observed in BJ, U-2 OS, U-87 MG and SH-SY5Y which are reported to be positive and not in other cell lines like MCF7, HT-29 and Raji using Anti-SPARC Polyclonal Antibody (Product # PA5-78178) in Western Blot. {RE}

Product Details

PA5-78178

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human SPARC. The exact sequence is proprietary.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.54 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2736653

Product Specific Information

Positive Control: NT2D1

Predicted Reactivity: Pig (85%), Sheep (94%), Bovine (97%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

SPARC gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The role of lymphoid tissue SPARC in the pathogenesis and response to treatment of multiple myeloma.

Frontiers in oncology

Aly NAR,Rizk S,Aboul Enein A,El Desoukey N,Zawam H,Ahmed M,El Shikh ME,Pitzalis C

PA5-78178 was used in Immunohistochemistry and Western Blotting to investigate the role of secreted protein acidic and rich in cysteine (SPARC) in the pathogenesis of multiple myeloma.

Wed Mar 22 00:00:00 UTC 2023

The role of lymphoid tissue SPARC in the pathogenesis and response to treatment of multiple myeloma.

Frontiers in oncology

Aly NAR,Rizk S,Aboul Enein A,El Desoukey N,Zawam H,Ahmed M,El Shikh ME,Pitzalis C

PA5-78178 was used in Immunohistochemistry and Western Blotting to investigate the role of secreted protein acidic and rich in cysteine (SPARC) in the pathogenesis of multiple myeloma.

Wed Mar 22 00:00:00 UTC 2023

The role of lymphoid tissue SPARC in the pathogenesis and response to treatment of multiple myeloma.

Frontiers in oncology

Aly NAR,Rizk S,Aboul Enein A,El Desoukey N,Zawam H,Ahmed M,El Shikh ME,Pitzalis C

PA5-78178 was used in Immunohistochemistry and Western Blotting to investigate the role of secreted protein acidic and rich in cysteine (SPARC) in the pathogenesis of multiple myeloma.

Wed Mar 22 00:00:00 UTC 2023

Bioinformatics

Protein Aliases: Basement membrane protein 40; Basement-membrane protein 40; BM-40; BM40; Cysteine rich protein; ON; Osteonectin; Secreted acidic cystein rich glycoprotein; Secreted acidic cystein-rich glycoprotein (osteonectin); secreted acidic cysteine rich glycoprotein; Secreted protein acidic and rich in cysteine; Secreted protein acidic and rich in cysteine (SPARC); secreted protein, acidic, cysteine-rich (osteonectin); SPARC

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Gene Aliases: BM-40; OI17; ON; SPARC

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UniProt ID: (Human) P09486, (Rat) P16975, (Mouse) P07214

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Entrez Gene ID: (Human) 6678, (Rat) 24791, (Mouse) 20692

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Function(s)

calcium ion binding protein binding collagen binding extracellular matrix binding metal ion binding

Process(es)

ossification negative regulation of endothelial cell proliferation signal transduction heart development response to gravity response to lead ion positive regulation of endothelial cell migration negative regulation of angiogenesis regulation of cell morphogenesis lung development response to lipopolysaccharide response to L-ascorbic acid response to cytokine wound healing regulation of cell proliferation response to peptide hormone response to ethanol response to cadmium ion inner ear development response to glucocorticoid response to cAMP response to calcium ion bone development cellular response to growth factor stimulus platelet degranulation receptor-mediated endocytosis extracellular matrix organization

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SPARC Polyclonal Antibody

SPARC Antibody (PA5-78178) in ICC/IF

Product Details

PA5-78178

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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