Invitrogen

SPARC Monoclonal Antibody (ON1-1)

7 Published Figures

33 References

View all (51) SPARC antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Published Figures (7)

SPARC Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 7

SPARC Antibody (33-5500) in ICC/IF

Asian journal of andrology 2020 - Figure 2 Basal expression, silencing, and overexpression of SPARC in PCa cell lines. (a) Representative image of western blot against SPARC in PCa cell lines 22Rv1, LNCaP, DU145 and PC3. (b) RT-qPCR of SPARC in PCa cell lines. DeltaDeltaCt was obtained after normalizing to PUM1 and PC3 cell lines ( n = 3). (c and d) PC3 cells were stable transduced with shRNA against SPARC (ShSPARC) or scramble (ShScr). LNC... View More

SPARC Antibody in Immunohistochemistry (IHC)

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Product Details

33-5500

Applications

Tested Dilution

Publications

Western Blot (WB)

5-10 µg/mL

View 4 publications 4 publications

Immunohistochemistry (IHC)

5-10 µg/mL

View 19 publications 19 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Flow Cytometry (Flow)

1 µg/mL

ELISA (ELISA)

Assay-dependent

View 2 publications 2 publications

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Human, Pig, Rabbit

Published species

Dog, Human, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

ON1-1

Immunogen

Full-length Bovine bone osteonectin (SPARC)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

2.0 mg/mL

Purification

Ion-exchange chromatography

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

no preservative

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533126

Product Specific Information

Source: Monoclonal antibody was obtained by fusing the mouse myeloma cell-line P3-X63-Ag8-U1 with spleen cells of BALB/c mouse after immunization with bovine bone osteonectin (SPARC). The monoclonal antibody was harvested from ascitic fluid.

Specificity: This antibody reacts with both bone derived and platelet derived Osteonectin (ELISA). This antibody does not react with non-activated platelet and reacts with thrombin-stimulated platelet (flow cytometric analysis). This antibody reacts with human and bovine Osteonectin (Western blot analysis). This antibody cross reacts with rabbit and porcine Osteonectin. (ELISA)

Storage: 4°C. This product does not contain preservative. The stock solution (2.0 mg/mL) should be stored in aliquots at -20°C for 1 year, or should be stored at 4°C for 6 months after adding 0.1% sodium azide. Avoid repeated freeze-thaw cycles.

Reconstitution: Dissolve the lyophilized antibody in 50 µL of distilled water (final concentration: 2.0 mg/mL). This solution can be used as a stock solution. If dilution is necessary for your application, dilute the stock solution with the following Dilution solution just prior to use. When the entire amount of antibody is to be used over a short time period, it may be dissolved directly in 500 µL or more of the Dilution solution. Be sure to store the antibody at a minimum concentration of 2.0 mg/mL. A lower antibody concentration may result in decreased stability. Reconstituted antibody solution should contain 0.1% sodium azide as a preservative when stored at 4°C.

Target Information

SPARC gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Quantitative Proteomics Identifies Secreted Diagnostic Biomarkers as well as Tumor-Dependent Prognostic Targets for Clear Cell Renal Cell Carcinoma.

Molecular cancer research : MCR

Senturk A,Sahin AT,Armutlu A,Kiremit MC,Acar O,Erdem S,Bagbudar S,Esen T,Tuncbag N,Ozlu N

33-5500 was used in Immunohistochemistry to propose a biomarker panel for the robust classification of ccRCC tumors into two main clusters, which significantly differed in patient outcome with an almost three times higher risk of death for cluster 1 tumors compared with cluster 2 tumors.

Sun Aug 01 00:00:00 UTC 2021

Quantitative Proteomics Identifies Secreted Diagnostic Biomarkers as well as Tumor-Dependent Prognostic Targets for Clear Cell Renal Cell Carcinoma.

Molecular cancer research : MCR

Senturk A,Sahin AT,Armutlu A,Kiremit MC,Acar O,Erdem S,Bagbudar S,Esen T,Tuncbag N,Ozlu N

Sun Aug 01 00:00:00 UTC 2021

Expression of Extracellular Matrix-Related Genes and Their Regulatory microRNAs in Problematic Colorectal Polyps.

Cancers

Žlajpah M,Boštjančič E,Tepeš B,Zidar N

33-5500 was used in Immunohistochemistry to analyse the expression of selected extracellular matrix (ECM)-related genes and proteins, and their regulatory microRNAs using RT-qPCR and immunohistochemistry in biopsies from 44 patients.

Fri Dec 11 00:00:00 UTC 2020

Expression of Extracellular Matrix-Related Genes and Their Regulatory microRNAs in Problematic Colorectal Polyps.

Cancers

Žlajpah M,Boštjančič E,Tepeš B,Zidar N

Fri Dec 11 00:00:00 UTC 2020

Effects of alloplastic graft material combined with a topical ozone application on calvarial bone defects in rats.

Folia morphologica

Laçin N,İzol BS,Gökalp Özkorkmaz E,Deveci B,Deveci E

33-5500 was used in Immunohistochemistry (Paraffin) to present the evaluation of the damage in the bone tissue resulting from a calvarial defect in rats and the efficiency of exposure to an ozone application with an alloplastic bone graft on the calvarial bone damage.

Tue Jul 13 00:00:00 UTC 2021

Secreted protein acidic and rich in cysteine (SPARC) induces epithelial-mesenchymal transition, enhancing migration and invasion, and is associated with high Gleason score in prostate cancer.

Asian journal of andrology

López-Moncada F,Torres MJ,Castellón EA,Contreras HR

33-5500 was used in Immunohistochemistry to show that secreted protein acidic and rich in cysteine (SPARC) is highly expressed in PCa tissues with a higher Gleason score.

Mon Aug 17 00:00:00 UTC 2020

Secreted protein acidic and rich in cysteine (SPARC) induces epithelial-mesenchymal transition, enhancing migration and invasion, and is associated with high Gleason score in prostate cancer.

Asian journal of andrology

López-Moncada F,Torres MJ,Castellón EA,Contreras HR

33-5500 was used in Immunohistochemistry to show that secreted protein acidic and rich in cysteine (SPARC) is highly expressed in PCa tissues with a higher Gleason score.

Mon Aug 17 00:00:00 UTC 2020

Secreted protein acidic and rich in cysteine (SPARC) induces epithelial-mesenchymal transition, enhancing migration and invasion, and is associated with high Gleason score in prostate cancer.

Asian journal of andrology

López-Moncada F,Torres MJ,Castellón EA,Contreras HR

33-5500 was used in Immunohistochemistry to show that secreted protein acidic and rich in cysteine (SPARC) is highly expressed in PCa tissues with a higher Gleason score.

Mon Aug 17 00:00:00 UTC 2020

Short-term use of resveratrol in alloplastic graft material applied with calvarial bone defects in rats.

Acta cirurgica brasileira

Laçin N,Deveci E

33-5500 was used in Immunohistochemistry to show that new bone formation in the calvaral defect region of rats can be induced by short term treatment of resveratrol in alloplastic graft material.

Thu Sep 12 00:00:00 UTC 2019

Ganoderma lucidum, a promising agent possessing antioxidant and anti-inflammatory effects for treating calvarial defects with graft application in rats.

Acta cirurgica brasileira

Laçin N,İzol SB,İpek F,Tuncer MC

33-5500 was used in Immunohistochemistry to show that, with its antioxidant and anti-inflammatory properties, G. Lucidum is an important factor in the treatment of calvarial bone defects.

Fri Dec 13 00:00:00 UTC 2019

The effect of graft application and allopurinol treatment on calvarial bone defect in rats1.

Acta cirurgica brasileira

Laçin N,İzol BS,Özkorkmaz EG,Deveci B,Tuncer MC

33-5500 was used in Immunohistochemistry to investigate the effects of allopurinol administration on osteoinductive reaction and bone development with graft material.

Mon Mar 18 00:00:00 UTC 2019

The effect of graft application and simvastatin treatment on tibial bone defect in rats. A histological and immunohistochemical study.

Acta cirurgica brasileira

Laçin N,İzol BS,Özkorkmaz EG,Deveci B,Tuncer MC

33-5500 was used in Immunohistochemistry to establish the increasing effect of simvastatin in bone turnover in rats, by increasing osteoinductive effect with graft.

Mon May 06 00:00:00 UTC 2019

Effects of formaldehyde on vascular endothelial growth factor, matrix metallopeptidase 2 and osteonectin levels in periodontal membrane and alveolar bone in rats.

Folia morphologica

Laçin N,İzol BS,Tuncer MC,Gökalp Özkorkmaz E,Deveci B,Deveci E

33-5500 was used in Immunohistochemistry to investigate if long term formaldehyde inhalation may effect periodontal membrane and alveolar bone loss leading to periodontitis.

Tue Jan 21 00:00:00 UTC 2020

Biochemical and immunohistochemical investigations on bone formation and remodelling in ovariectomised rats with tamoxifen citrate administration.

Folia morphologica

Baloğlu M,Gökalp Özkorkmaz E

33-5500 was used in Immunohistochemistry to observe that Osteonectin promotes bone formation by influencing collagen fiber formation, extracellular matrix development, osteoblast differentiation and the capacity to affect osteoclast activity.

Tue Jul 07 00:00:00 UTC 2020

Diagnostic role of circulating extracellular matrix-related proteins in non-small cell lung cancer.

BMC cancer

Andriani F,Landoni E,Mensah M,Facchinetti F,Miceli R,Tagliabue E,Giussani M,Callari M,De Cecco L,Colombo MP,Roz L,Pastorino U,Sozzi G

33-5500 was used in Western Blotting to study the effects of COL11A1, COL10A1 and SPARC proteins in plasma samples of lung cancer patients and in healthy heavy-smokers controls and test whether such measurements have diagnostic or prognostic value.

Tue Sep 18 00:00:00 UTC 2018

Diagnostic role of circulating extracellular matrix-related proteins in non-small cell lung cancer.

BMC cancer

Andriani F,Landoni E,Mensah M,Facchinetti F,Miceli R,Tagliabue E,Giussani M,Callari M,De Cecco L,Colombo MP,Roz L,Pastorino U,Sozzi G

Tue Sep 18 00:00:00 UTC 2018

Attachment and growth of dental pulp stem cells on dentin in presence of extra calcium.

Archives of oral biology

Özdal-Kurt F,Şen BH,Tuğlu I,Vatansever S,Türk BT,Deliloğlu-Gürhan I

33-5500 was used in immunocytochemistry to characterize the growth and attachment of dental pulp stem cells on dentin with extra calcium

Mon Aug 01 00:00:00 UTC 2016

The effect of different implant biomaterials on the behavior of canine bone marrow stromal cells during their differentiation into osteoblasts.

Biotechnic & histochemistry : official publication of the Biological Stain Commission

Özdal-Kurt F,Tuğlu I,Vatansever HS,Tong S,Şen BH,Deliloğlu-Gürhan SI

33-5500 was used in immunohistochemistry to elucidate the behavior of canine bone marrow stromal cells during differentiation into osteoblasts utilizing different implant biomaterials

Mon Aug 01 00:00:00 UTC 2016

Stromal Caveolin-1 Is Associated With Response and Survival in a Phase II Trial of nab-Paclitaxel With Carboplatin for Advanced NSCLC Patients.

Clinical lung cancer

Bertino EM,Williams TM,Nana-Sinkam SP,Shilo K,Chatterjee M,Mo X,Rahmani M,Phillips GS,Villalona-Calero MA,Otterson GA

33-5500 was used in immunohistochemistry to evaluate carboplatin with nanoparticle albumin-bound-paclitaxel as first-line therapy for advanced non-small-cell lung cancer in a phase II trail.

Sun Nov 01 00:00:00 UTC 2015

The effect of autologous bone marrow stromal cells differentiated on scaffolds for canine tibial bone reconstruction.

Biotechnic & histochemistry : official publication of the Biological Stain Commission

Özdal-Kurt F,Tuğlu I,Vatansever HS,Tong S,Deliloğlu-Gürhan SI

33-5500 was used in immunohistochemistry to assess the use of cultured autologous bone marrow stromal cells on different scaffolds for healing defects in the tibias of dogs.

Thu Sep 01 00:00:00 UTC 2016

A Magnetic Bead-Based Sensor for the Quantification of Multiple Prostate Cancer Biomarkers.

PloS one

Jokerst JV,Chen Z,Xu L,Nolley R,Chang E,Mitchell B,Brooks JD,Gambhir SS

33-5500 was used in ELISA to optimize a method to distinguish benign prostatic hypertrophy from prostate cancer.

Mon Mar 28 00:00:00 UTC 2016

Vasculogenic mimicry: a new prognostic sign of human osteosarcoma.

Human pathology

Ren K,Yao N,Wang G,Tian L,Ma J,Shi X,Zhang L,Zhang J,Zhou X,Zhou G,Wu S,Sun X

33-5500 was used in immunohistochemistry to evaluate the vasculogenic mimicry as a prognostic marker of human osteosarcoma

Wed Oct 01 00:00:00 UTC 2014

Stromal responses among common carcinomas correlated with clinicopathologic features.

Clinical cancer research : an official journal of the American Association for Cancer Research

Chen JL,Espinosa I,Lin AY,Liao OY,van de Rijn M,West RB

33-5500 was used in immunohistochemistry to test if the desmoid-type fibromatosis signature is specific to breast cancer or if it is found in multiple types of cancer.

Sun Sep 15 00:00:00 UTC 2013

Neoplastic and stromal cells contribute to an extracellular matrix gene expression profile defining a breast cancer subtype likely to progress.

PloS one

Triulzi T,Casalini P,Sandri M,Ratti M,Carcangiu ML,Colombo MP,Balsari A,Ménard S,Orlandi R,Tagliabue E

33-5500 was used in immunohistochemistry (paraffin) to examine the characteristics of the extracellular matrix and its impact on tumor progression.

Tue Aug 13 00:00:00 UTC 2013

Elevated plasma SPARC levels are associated with insulin resistance, dyslipidemia, and inflammation in gestational diabetes mellitus.

PloS one

Xu L,Ping F,Yin J,Xiao X,Xiang H,Ballantyne CM,Wu H,Li M

33-5500 was used in ELISA to test if the concentrations of SPARC are altered in patients with gestational diabetes mellitus.

Mon Sep 29 00:00:00 UTC 2014

Effects of bisphosphonates on sutural bone formation and relapse: A histologic and immunohistochemical study.

American journal of orthodontics and dentofacial orthopedics : official publication of the American Association of Orthodontists, its constituent societies, and the American Board of Orthodontics

Oztürk F,Babacan H,Inan S,Gümüş C

Fri Jul 01 00:00:00 UTC 2011

A physical mechanism for coupling bone resorption and formation in adult human bone.

The American journal of pathology

Andersen TL,Sondergaard TE,Skorzynska KE,Dagnaes-Hansen F,Plesner TL,Hauge EM,Plesner T,Delaisse JM

33-5500 was used in immunohistochemistry to investigate the molecular mechanism of bone remodeling with osteoclasts and osteoblasts

Thu Jan 01 00:00:00 UTC 2009

Markers aiding the diagnosis of chondroid tumors: an immunohistochemical study including osteonectin, bcl-2, cox-2, actin, calponin, D2-40 (podoplanin), mdm-2, CD117 (c-kit), and YKL-40.

APMIS : acta pathologica, microbiologica, et immunologica Scandinavica

Daugaard S,Christensen LH,Høgdall E

33-5500 was used in immunohistochemistry - paraffin section to identify immunohistochemical markers to aid chondroid tumor diagnosis

Wed Jul 01 00:00:00 UTC 2009

Multiple genes are hypermethylated in intraductal papillary mucinous neoplasms of the pancreas.

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

Hong SM,Kelly D,Griffith M,Omura N,Li A,Li CP,Hruban RH,Goggins M

33-5500 was used in immunohistochemistry - paraffin section to examine DNA methylation in intraductal papillary mucinous neoplasms

Mon Dec 01 00:00:00 UTC 2008

The nonsteroidal anti-inflammatory drug NS398 reactivates SPARC expression via promoter demethylation to attenuate invasiveness of lung cancer cells.

Experimental biology and medicine (Maywood, N.J.)

Pan MR,Chang HC,Chuang LY,Hung WC

33-5500 was used in immunocytochemistry and western blot to investigate how nonsteroidal anti-inflammatory drugs alter metastasis-related genes

Tue Apr 01 00:00:00 UTC 2008

Peritumoral fibroblast SPARC expression and patient outcome with resectable pancreatic adenocarcinoma.

Journal of clinical oncology : official journal of the American Society of Clinical Oncology

Infante JR,Matsubayashi H,Sato N,Tonascia J,Klein AP,Riall TA,Yeo C,Iacobuzio-Donahue C,Goggins M

33-5500 was used in immunohistochemistry - paraffin section to assess the prognostic significance of tumor and peritumoral secreted protein acidic and rich in cysteine expression in patients with pancreatic adenocarcinoma

Sat Jan 20 00:00:00 UTC 2007

SPARC expression is associated with impaired tumor growth, inhibited angiogenesis and changes in the extracellular matrix.

International journal of cancer

Chlenski A,Liu S,Guerrero LJ,Yang Q,Tian Y,Salwen HR,Zage P,Cohn SL

33-5500 was used in immunohistochemistry and western blot to study the role of SPARC expression in impaired tumor growth, inhibited angiogenesis and extracellular matrix changes

Sun Jan 15 00:00:00 UTC 2006

Gene expression in the normal adult human kidney assessed by complementary DNA microarray.

Molecular biology of the cell

Higgins JP,Wang L,Kambham N,Montgomery K,Mason V,Vogelmann SU,Lemley KV,Brown PO,Brooks JD,van de Rijn M

Sun Feb 01 00:00:00 UTC 2004

Gene expression profiling of normal human pulmonary fibroblasts following coculture with non-small-cell lung cancer cells reveals alterations related to matrix degradation, angiogenesis, cell growth and survival.

Oncogene

Fromigué O,Louis K,Dayem M,Milanini J,Pages G,Tartare-Deckert S,Ponzio G,Hofman P,Barbry P,Auberger P,Mari B

Thu Nov 20 00:00:00 UTC 2003

SPARC/osteonectin is a frequent target for aberrant methylation in pancreatic adenocarcinoma and a mediator of tumor-stromal interactions.

Oncogene

Sato N,Fukushima N,Maehara N,Matsubayashi H,Koopmann J,Su GH,Hruban RH,Goggins M

Thu Aug 07 00:00:00 UTC 2003

Bioinformatics

Protein Aliases: Basement membrane protein 40; Basement-membrane protein 40; BM-40; BM40; Cysteine rich protein; ON; Osteonectin; Secreted acidic cystein rich glycoprotein; Secreted protein acidic and rich in cysteine; Secreted protein acidic and rich in cysteine (SPARC); secreted protein, acidic, cysteine-rich (osteonectin); SPARC

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Gene Aliases: BM-40; BOS_8355; OI17; ON; SPARC

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UniProt ID: (Bovine) P13213, (Human) P09486, (Pig) P20112

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Entrez Gene ID: (Bovine) 282077, (Human) 6678, (Pig) 595124, (Rabbit) 100009355

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Function(s)

calcium ion binding extracellular matrix binding protein binding collagen binding

Process(es)

signal transduction regulation of cell proliferation bone development cellular response to growth factor stimulus ossification negative regulation of endothelial cell proliferation platelet degranulation receptor-mediated endocytosis heart development response to gravity response to lead ion positive regulation of endothelial cell migration negative regulation of angiogenesis regulation of cell morphogenesis extracellular matrix organization lung development response to lipopolysaccharide response to L-ascorbic acid response to cytokine wound healing response to peptide hormone response to ethanol response to cadmium ion inner ear development response to glucocorticoid response to cAMP response to calcium ion

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SPARC Monoclonal Antibody (ON1-1)

SPARC Antibody (33-5500) in ICC/IF

Product Details

33-5500

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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