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Invitrogen
SPARC Recombinant Rabbit Monoclonal Antibody (PD00-57)
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FIGURE: 1 / 2
SPARC Antibody (MA5-50724) in IHC (P)
Immunohistochemistry of SPARC in paraffin-embedded human malignant melanoma tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with SPARC recombinant monoclonal antibody (Product # MA5-50724) with a dilution of 1:500 for 1 hou... View More
Product Details
MA5-50724
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
PD00-57
Immunogen
Synthetic peptide aa 18-303/303.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3093015
Product Specific Information
Positive control: Human malignant melanoma tissue, human glioma tissue.
Predicted band size: 35 kDa
Subcellular Location: Secreted, extracellular space, extracellular matrix, basement membrane.
Target Information
SPARC gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Basement membrane protein 40; Basement-membrane protein 40; BM-40; BM40; Cysteine rich protein; ON; Osteonectin; Secreted acidic cystein rich glycoprotein; Secreted protein acidic and rich in cysteine; Secreted protein acidic and rich in cysteine (SPARC); secreted protein, acidic, cysteine-rich (osteonectin); SPARC
Gene Aliases: BM-40; OI17; ON; SPARC
UniProt ID: (Human) P09486
Entrez Gene ID: (Human) 6678
ossification
negative regulation of endothelial cell proliferation
platelet degranulation
receptor-mediated endocytosis
signal transduction
heart development
response to gravity
response to lead ion
positive regulation of endothelial cell migration
negative regulation of angiogenesis
regulation of cell morphogenesis
extracellular matrix organization
lung development
response to lipopolysaccharide
response to L-ascorbic acid
response to cytokine
wound healing
response to peptide hormone
response to ethanol
response to cadmium ion
inner ear development
response to glucocorticoid
response to cAMP
response to calcium ion
bone development
cellular response to growth factor stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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