Proteintech

SPATA22 Polyclonal Antibody

SPATA22 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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SPATA22 Antibody (16989-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded mouse testis tissue slide using 16989-1-AP (SPATA22 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

SPATA22 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

16989-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 7 publications 7 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SPATA22 Fusion Protein Ag10591 (1-363 aa encoded by BC029483)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MKRSLNENS ARSTAGCLPV PLFNQKKRNR QPLTSNPLKD DSGISTPSDN YDFPPLPTDW AWEAVNPELA PVMKTVDTGQ IPHSVSRPLR SQDSVFNSIQ SNTGRSQGGW SYRDGNKNTS LKTWNKNDFK PQCKRTNLVA NDGKNSCPMS SGAQQQKQLR TPEPPNLSRN KETELLRQTH SSKISGCTMR GLDKNSALQT LKPNFQQNQY KKQMLDDIPE DNTLKETSLY QLQFKEKASS LRIISAVIES MKYWREHAQK TVLLFEVLAV LDSAVTPGPY YSKTFLMRDG KNTLPCVFYE IDRELPRLIR GRVHRCVGNY DQKKNIFQCV SVRPASVSEQ KTFQAFVKIA DVEMQYYINV MNET (1-363 aa encoded by BC029483)

Target Information

SPATA22 is highly expressed in adult testis. The specific function of SPATA22 is not yet known.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A truncating variant of RAD51B associated with primary ovarian insufficiency provides insights into its meiotic and somatic functions.

Cell death and differentiation

Franca MM,Condezo YB,Elzaiat M,Felipe-Medina N,Sánchez-Sáez F,Muñoz S,Sainz-Urruela R,Martín-Hervás MR,García-Valiente R,Sánchez-Martín MA,Astudillo A,Mendez J,Llano E,Veitia RA,Mendonca BB,Pendás AM

Thu Dec 01 00:00:00 UTC 2022

SCF ubiquitin E3 ligase regulates DNA double-strand breaks in early meiotic recombination.

Nucleic acids research

Guan Y,Lin H,Leu NA,Ruthel G,Fuchs SY,Busino L,Luo M,Wang PJ

Fri May 20 00:00:00 UTC 2022

Nutrient restriction synergizes with retinoic acid to induce mammalian meiotic initiation in vitro.

Nature communications

Zhang X,Gunewardena S,Wang N

Fri Mar 19 00:00:00 UTC 2021

Proteostasis regulated by testis-specific ribosomal protein RPL39L maintains mouse spermatogenesis.

iScience

Zou Q,Yang L,Shi R,Qi Y,Zhang X,Qi H

Fri Dec 17 00:00:00 UTC 2021

The BRCA2-MEILB2-BRME1 complex governs meiotic recombination and impairs the mitotic BRCA2-RAD51 function in cancer cells.

Nature communications

Zhang J,Gurusaran M,Fujiwara Y,Zhang K,Echbarthi M,Vorontsov E,Guo R,Pendlebury DF,Alam I,Livera G,Emmanuelle M,Wang PJ,Nandakumar J,Davies OR,Shibuya H

Tue Apr 28 00:00:00 UTC 2020

A missense in HSF2BP causing primary ovarian insufficiency affects meiotic recombination by its novel interactor C19ORF57/BRME1.

eLife

Felipe-Medina N,Caburet S,Sánchez-Sáez F,Condezo YB,de Rooij DG,Gómez-H L,Garcia-Valiente R,Todeschini AL,Duque P,Sánchez-Martin MA,Shalev SA,Llano E,Veitia RA,Pendás AM

Wed Aug 26 00:00:00 UTC 2020

Meiosis-Specific C19orf57/4930432K21Rik/BRME1 Modulates Localization of RAD51 and DMC1 to DSBs in Mouse Meiotic Recombination.

Cell reports

Takemoto K,Tani N,Takada-Horisawa Y,Fujimura S,Tanno N,Yamane M,Okamura K,Sugimoto M,Araki K,Ishiguro KI

Tue May 26 00:00:00 UTC 2020

A cell-based high-content screen identifies isocotoin as a small molecule inhibitor of the meiosis-specific MEIOB-SPATA22 complex†.

Biology of reproduction

Xu Y,Liu R,Leu NA,Zhang L,Ibragmova I,Schultz DC,Wang PJ

Tue Aug 04 00:00:00 UTC 2020

A cell-based high-content screen identifies isocotoin as a small molecule inhibitor of the meiosis-specific MEIOB-SPATA22 complex†.

Biology of reproduction

Xu Y,Liu R,Leu NA,Zhang L,Ibragmova I,Schultz DC,Wang PJ

Tue Aug 04 00:00:00 UTC 2020

A meiosis-specific BRCA2 binding protein recruits recombinases to DNA double-strand breaks to ensure homologous recombination.

Nature communications

Zhang J,Fujiwara Y,Yamamoto S,Shibuya H

Wed Feb 13 00:00:00 UTC 2019

A meiosis-specific BRCA2 binding protein recruits recombinases to DNA double-strand breaks to ensure homologous recombination.

Nature communications

Zhang J,Fujiwara Y,Yamamoto S,Shibuya H

Wed Feb 13 00:00:00 UTC 2019

MEIOB exhibits single-stranded DNA-binding and exonuclease activities and is essential for meiotic recombination.

Nature communications

Luo M,Yang F,Leu NA,Landaiche J,Handel MA,Benavente R,La Salle S,Wang PJ

Tue Jul 15 00:00:00 UTC 2014

MEIOB exhibits single-stranded DNA-binding and exonuclease activities and is essential for meiotic recombination.

Nature communications

Luo M,Yang F,Leu NA,Landaiche J,Handel MA,Benavente R,La Salle S,Wang PJ

Tue Jul 15 00:00:00 UTC 2014

Spata22, a novel vertebrate-specific gene, is required for meiotic progress in mouse germ cells.

Biology of reproduction

La Salle S,Palmer K,O'Brien M,Schimenti JC,Eppig J,Handel MA

Wed Feb 01 00:00:00 UTC 2012

Spata22, a novel vertebrate-specific gene, is required for meiotic progress in mouse germ cells.

Biology of reproduction

La Salle S,Palmer K,O'Brien M,Schimenti JC,Eppig J,Handel MA

Wed Feb 01 00:00:00 UTC 2012

Spata22, a novel vertebrate-specific gene, is required for meiotic progress in mouse germ cells.

Biology of reproduction

La Salle S,Palmer K,O'Brien M,Schimenti JC,Eppig J,Handel MA

Wed Feb 01 00:00:00 UTC 2012

Spata22, a novel vertebrate-specific gene, is required for meiotic progress in mouse germ cells.

Biology of reproduction

La Salle S,Palmer K,O'Brien M,Schimenti JC,Eppig J,Handel MA

Wed Feb 01 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: Spermatogenesis-associated protein 22; testicular tissue protein Li 186; Testis development protein NYD-SP20

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Gene Aliases: Gm882; NYD-SP20; NYDSP20; Repro42; RGD1565378; Sgn; SPATA22

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UniProt ID: (Human) Q8NHS9, (Mouse) Q5SV06

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Entrez Gene ID: (Human) 84690, (Mouse) 380709, (Rat) 360565

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Function(s)

protein binding

Process(es)

meiotic DNA repair synthesis synapsis gamete generation fertilization meiotic cell cycle regulation of meiotic cell cycle reproductive system development

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SPATA22 Polyclonal Antibody

SPATA22 Antibody (16989-1-AP) in IHC (P)

Product Details

16989-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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