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Invitrogen
SPC24 Polyclonal Antibody
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FIGURE: 1 / 1
SPC24 Antibody (PA5-95812) in WB
Western blot analysis of SPC24 in HeLa cells. Samples were incubated with SPC24 Polyclonal antibody (Product # PA5-95812) using a dilution of 1:2,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Product Details
PA5-95812
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-197 of human SPC24 (NP_8723191)
Conjugate
Form
Liquid
Concentration
2.12 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2807614
Product Specific Information
Immunogen sequence: MAAFRDIEEV SQGLLSLLGA NRAEAQQRRL LGRHEQVVER LLETQDGAEK QLREILTMEK EVAQSLLNAK EQVHQGGVEL QQLEAGLQEA GEEDTRLKAS LLQLTRELEE LKEIEADLER QEKEVDEDTT VTIPSAVYVA QLYHQVSKIE WDYECEPGMV KGIHHGPSVA QPIHLDSTQL SRKFISDYLW SLVDTEW; Positive Samples: Jurkat, HeLa, 293T; Cellular Location: Chromosome, Nucleus, centromere, kinetochore
Target Information
Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hSpc24; Kinetochore protein Spc24; SPC24, NDC80 kinetochore complex component, homolog; spindle pole body component 24 homolog
Gene Aliases: 2410030K01Rik; AV109292; SPBC24; SPC24
UniProt ID: (Human) Q8NBT2, (Mouse) Q9D083
Entrez Gene ID: (Human) 147841, (Mouse) 67629
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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