Proteintech

SPDEF Polyclonal Antibody

FIGURE: 1 / 4

SPDEF Antibody (11467-1-AP) in WB

MCF-7 cells were subjected to SDS PAGE followed by western blot with 11467-1-AP ( SPDEF Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Product Details

11467-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SPDEF Fusion Protein Ag2020 (1-335 aa encoded by BC021299)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.31 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MGSASPGLS SVSPSHLLLP PDTVSRTGLE KAAAGAVGLE RRDWSPSPPA TPEQGLSAFY LSYFDMLYPE DSSWAAKAPG ASSREEPPEE PEQCPVIDSQ APAGSLDLVP GGLTLEEHSL EQVQSMVVGE VLKDIETACK LLNITADPMD WSPSNVQKWL LWTEHQYRLP PMGKAFQELA GKELCAMSEE QFRQRSPLGG DVLHAHLDIW KSAAWMKERT SPGAIHYCAS TSEESWTDSE VDSSCSGQPI HLWQFLKELL LKPHSYGRFI RWLNKEKGIF KIEDSAQVAR LWGIRKNRPA MNYDKLSRSI RQYYKKGIIR KPDISQRLVY QFVHPI (1-335 aa encoded by B C021299)

Target Information

PDEF (prostate-derived Ets factor) is a 37.5-kDa protein that acts as an androgen-independent transcriptional activator for the PSA (prostate specific antigen) promoter. It also interacts directly with the DNA binding domain of androgen receptor and enhances androgen-mediated activation of the PSA promoter. PDEF was shown to have transcript levels 192-fold higher in the peripheral blood of some breast cancer patients in comparison with normal individuals. PDEF mRNA is also frequently over-expressed in human breast tumors as well as in human ovarian tumors. In ovarian tumors, PDEF expression seems to down-modulate malignant potential, and thus provides a rationale to screen for drugs that induce PDEF expression in epithelial ovarian tumors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Shaoyao decoction restores the mucus layer in mice with DSS-induced colitis by regulating Notch signaling pathway.

Journal of ethnopharmacology

Fang YX,Liu YQ,Hu YM,Yang YY,Zhang DJ,Jiang CH,Wang JH,Zhang J

Tue May 23 00:00:00 UTC 2023

EPA and DHA differentially coordinate the crosstalk between host and gut microbiota and block DSS-induced colitis in mice by a reinforced colonic mucus barrier.

Food & function

Fang J,Zhang Z,Cheng Y,Yang H,Zhang H,Xue Z,Lu S,Dong Y,Song C,Zhang X,Zhou Y

Wed Apr 20 00:00:00 UTC 2022

Gut microbiota-derived butyrate regulates gut mucus barrier repair by activating the macrophage/WNT/ERK signaling pathway.

Clinical science (London, England : 1979)

Liang L,Liu L,Zhou W,Yang C,Mai G,Li H,Chen Y

Fri Feb 25 00:00:00 UTC 2022

Gut microbiota-derived butyrate regulates gut mucus barrier repair by activating the macrophage/WNT/ERK signaling pathway.

Clinical science (London, England : 1979)

Liang L,Liu L,Zhou W,Yang C,Mai G,Li H,Chen Y

Fri Feb 25 00:00:00 UTC 2022

Resveratrol inhibits MUC5AC expression by regulating SPDEF in lung cancer cells.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Lin YH,Zhu LY,Yang YQ,Zhang ZH,Chen QG,Sun YP,Bi JJ,Luo XM,Ni ZH,Wang XB

Sun Aug 01 00:00:00 UTC 2021

Resveratrol inhibits MUC5AC expression by regulating SPDEF in lung cancer cells.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Lin YH,Zhu LY,Yang YQ,Zhang ZH,Chen QG,Sun YP,Bi JJ,Luo XM,Ni ZH,Wang XB

Sun Aug 01 00:00:00 UTC 2021

Compromised Ileal Mucus Barrier Due to Impaired Epithelial Homeostasis Caused by Notch1 Signaling in Cirrhotic Rats.

Digestive diseases and sciences

Zhang L,Tai Y,Tang S,Zhao C,Tong H,Gao J,Tang C

Fri Jan 01 00:00:00 UTC 2021

A modular master regulator landscape controls cancer transcriptional identity.

Cell

Paull EO,Aytes A,Jones SJ,Subramaniam PS,Giorgi FM,Douglass EF,Tagore S,Chu B,Vasciaveo A,Zheng S,Verhaak R,Abate-Shen C,Alvarez MJ,Califano A

Thu Jan 21 00:00:00 UTC 2021

Neuroplastin-β mediates S100A8/A9-induced lung cancer disseminative progression.

Molecular carcinogenesis

Sumardika IW,Chen Y,Tomonobu N,Kinoshita R,Ruma IMW,Sato H,Kondo E,Inoue Y,Yamauchi A,Murata H,Yamamoto KI,Tomida S,Shien K,Yamamoto H,Soh J,Futami J,Putranto EW,Hibino T,Nishibori M,Toyooka S,Sakaguchi M

Sat Jun 01 00:00:00 UTC 2019

Bioinformatics

Protein Aliases: Prostate epithelium-specific Ets transcription factor; prostate specific ets transcription factor; Prostate-derived Ets factor; Prostate-specific Ets; SAM pointed domain-containing Ets transcription factor

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Gene Aliases: bA375E1.3; PDEF; PSE; SPDEF

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UniProt ID: (Human) O95238, (Mouse) Q9WTP3

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Entrez Gene ID: (Human) 25803, (Mouse) 30051, (Rat) 689210

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Function(s)

RNA polymerase II transcription factor activity, sequence-specific DNA binding sequence-specific DNA binding DNA binding transcription factor activity, sequence-specific DNA binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated multicellular organism development negative regulation of cell fate commitment positive regulation of cell fate commitment cell differentiation positive regulation of apoptotic process positive regulation of transcription from RNA polymerase II promoter lung goblet cell differentiation intestinal epithelial cell development regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SPDEF Polyclonal Antibody

SPDEF Antibody (11467-1-AP) in WB

Product Details

11467-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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