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Invitrogen
SPDYA Polyclonal Antibody
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FIGURE: 1 / 4
SPDYA Antibody (PA1-16959) in IHC (P)
Immunohistochemical analysis of SPDYA in immersion fixed paraffin-embedded sections of human testis. Samples were incubated in SPDYA polyclonal antibody (Product # PA1-16959) using a dilution of 1:300 for 1 hour at room temperature followed by the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized t... View More
Product Details
PA1-16959
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Sheep,
Rat
Published species
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues 200-286 of human Speedy/Ringo.
Conjugate
Form
Liquid
Concentration
1.1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/tris phosphate, pH 7-8
Contains
0.1% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2195543
Product Specific Information
PA1-16959 is specific for the ~36 kDa Speedy/Ringo protein (isoform 2).
After a longer exposure, a 31 kDa band can be detected (isoform 1).
There is also a non-specific band detected at ~58 kDa.Suggested positive control: mouse testis lysate, mouse testis protein.
Target Information
Speedy A, also known as SPDYA, SPDY1, Ringo3 or SPY1, is a 313 amino acid protein that localizes to the nucleus and belongs to the speedy/ringo family. Expressed at high levels in testis and at lower levels in brain, kidney, heart, bone marrow, colon, lung, liver spleen and placenta, Speedy A functions to regulate the G1/S phase transition of the cell cycle, specifically by binding to and activating Cdc2 p34, Cdk2 and p27. Additionally, Speedy A mediates cell survival during DNA damage, suggesting that Speedy A plays a role in proper cell cycle progression throughout the life of the cell. Multiple isoforms of Speedy A exist due to alternative splicing events. The gene encoding Speedy A maps to human chromosome 2, which encodes over 1,400 genes and comprises nearly 8% of the human genome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: GS4; hSpy/Ringo A; MLZ-465; mSpy/Ringo A; Protein expressed in male leptotene and zygotene spermatocytes 465; Rapid inducer of G2/M progression in oocytes A; RINGO A; SPDYA; spdya {ECO:0000250|UniProtKB:Q5MJ70}; speedy A; speedy A1; speedy A2; speedy homolog 1; speedy homolog A; Speedy protein A; Speedy-1; speedy/ringo; spermatogenesis related protein Gs4; Spy1
Gene Aliases: 4921517J08Rik; 4930548B21Rik; GS4; Lm23; MLZ-465; RINGO3; RINGOA; SPDY1; SPDYA; SPY1
UniProt ID: (Human) Q5MJ70, (Mouse) Q5IBH7, (Rat) Q8R496
Entrez Gene ID: (Human) 245711, (Sheep) 101123454, (Mouse) 70891, (Rat) 192209
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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