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Invitrogen
SPG7 Polyclonal Antibody
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FIGURE: 1 / 1
SPG7 Antibody (PA5-87745) in WB
Western blot analysis of SPG7 in extracts of various cell lines. Samples were incubated with SPG7 Polyclonal antibody (Product # PA5-87745) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-87745
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human SPG7 (NP_0031101)
Conjugate
Form
Liquid
Concentration
0.32 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2804382
Product Specific Information
Immunogen sequence: MAVLLLLLRA LRRGPGPGPR PLWGPGPAWS PGFPARPGRG RPYMASRPPG DLAEAGGRAL QSLQLRLLTP TFEGINGLLL KQHLVQNPVR LWQLLGGTFY FNTSRLKQKN KEKDKSKGKA PEEDEEERRR RERDDQMYRE RLRTLLVIAV VMSLLNALST SGGSISWNDF VHEMLAKGEV QRVQVVPESD VVEVYLHPGA VVFGRPRLAL MYRMQVANID KFEEKLRAAE DELNIEAKDR IPVSYKRTGF; Positive Samples: U-87MG, MCF7, HT-29, K-562, Mouse brain, Mouse kidney, Mouse liver; Cellular Location: Mitochondrion membrane, Multi-pass membrane protein
Target Information
This gene encodes a mitochondrial metalloprotease protein that is a member of the AAA family. Members of this protein family share an ATPase domain and have roles in diverse cellular processes including membrane trafficking, intracellular motility, organelle biogenesis, protein folding, and proteolysis. Mutations in this gene cause autosomal recessive spastic paraplegia 7. Two transcript variants encoding distinct isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cell adhesion regulator; Cell matrix adhesion regulator; Mitochondrial inner membrane m-AAA protease component paraplegin; Paraplegin; paraplegin, isoform 1; spastic paraplegia 7 (pure and complicated autosomal recessive); Spastic paraplegia 7 protein
Gene Aliases: AI452278; AU015315; CAR; CMAR; PGN; SPG5C; SPG7
UniProt ID: (Human) Q9UQ90, (Mouse) Q3ULF4
Entrez Gene ID: (Human) 6687, (Mouse) 234847
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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