Proteintech

SPHK2 Polyclonal Antibody

SPHK2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 21

SPHK2 Antibody (17096-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol) fixed HEK-293 cells using 17096-1-AP (SPHK2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

SPHK2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SPHK2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

17096-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:6,000

View 26 publications 26 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 3 publications 3 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Not applicable (N/A)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SPHK2 Fusion Protein Ag10569 (266-618 aa encoded by BC010671)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: LNCSLLLCR GGGHPLDLLS VTLASGSRCF SFLSVAWGFV SDVDIQSERF RALGSARFTL GTVLGLATLH TYRGRLSYLP ATVEPASPTP AHSLPRAKSE LTLTPDPAPP MAHSPLHRSV SDLPLPLPQP ALASPGSPEP LPILSLNGGG PELAGDWGGA GDAPLSPDPL LSSPPGSPKA ALHSPVSEGA PVIPPSSGLP LPTPDARVGA STCGPPDHLL PPLGTPLPPD WVTLEGDFVL MLAISPSHLG ADLVAAPHAR FDDGLVHLCW VRSGISRAAL LRLFLAMERG SHFSLGCPQL GYAAARAFRL EPLTPRGVLT VDGEQVEYGP LQAQMHPGIG TLLTGPPGCP GREP (266-618 aa encoded by B C010671)

Target Information

SPHK2, along with SPHK1, catalyzes the phosphorylation of sphingosine producing the signaling molecule sphingosine-1-phosphate (S1P). S1P signals through G-protein coupled receptors and controls diverse cellular processes. Inhibitors of SPHKs are viewed as potential therapeutics for the treatment of cancer. SPHK2 is an important signaling molecule with intra- and extracellular functions and has been implicated in regulating cell differentiation, calcium mobilization from intracellular stores, and apoptosis. The cell surface receptors for S1P are the EDG family of G protein-coupled receptors (S1P Receptors). These receptors couple to multiple G proteins (e.g. S1P1 couples to Gi whereas S1P2 and S1P3 couple to Gq, G13 in addition to Gi) and regulate a extremely wide raange of cellular events including cell motility, survival, apoptosis, migration and cell-cell interaction. Important roles for S1P have also been reported in regulation of cardiogenesis, vascular maturation, oocyte survival, immune cell trafficking, cells of the neuronal system and bone cells. S1P levels are regulated by the activity of Sphk (Sphk1 and Sphk2).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Study on the action mechanism of Buyang Huanwu Decoction against ischemic stroke based on S1P/S1PR1/PI3K/Akt signaling pathway.

Journal of ethnopharmacology

Liu W,Zhou X,Zeng K,Nie C,Huang J,Zhu L,Pei D,Zhang Y

Thu Aug 10 00:00:00 UTC 2023

Study on the action mechanism of Buyang Huanwu Decoction against ischemic stroke based on S1P/S1PR1/PI3K/Akt signaling pathway.

Journal of ethnopharmacology

Liu W,Zhou X,Zeng K,Nie C,Huang J,Zhu L,Pei D,Zhang Y

Thu Aug 10 00:00:00 UTC 2023

Sphingosine kinase 2 regulates insulin receptor trafficking in hepatocytes.

Experimental biology and medicine (Maywood, N.J.)

Aji G,Jiang S,Obulkasim H,Lu Z,Wang W,Xia P

Sun Jan 01 00:00:00 UTC 2023

Enzymatic independent role of sphingosine kinase 2 in regulating the expression of type I interferon during influenza A virus infection.

PLoS pathogens

Xu M,Xia S,Wang M,Liu X,Li X,Chen W,Wang Y,Li H,Xia C,Chen J,Wu J

Thu Sep 01 00:00:00 UTC 2022

FTY720 Reduces Lipid Accumulation by Upregulating ABCA1 through Liver X Receptor and Sphingosine Kinase 2 Signaling in Macrophages.

International journal of molecular sciences

Tachibana K,Kusumoto K,Ogawa M,Ando H,Shimizu T,Ishima Y,Ishida T,Okuhira K

Wed Nov 23 00:00:00 UTC 2022

Inhibition of Sphingosine Kinase Activity Enhances Immunogenic Cell Surface Exposure of Calreticulin Induced by the Synthetic Cannabinoid 5-epi-CP-55,940.

Cannabis and cannabinoid research

Hengst JA,Nduwumwami AJ,Raup-Konsavage WM,Vrana KE,Yun JK

Sat Oct 01 00:00:00 UTC 2022

Chronic treatment with serelaxin mitigates adverse remodeling in a murine model of ischemic heart failure and modulates bioactive sphingolipid signaling.

Scientific reports

Devarakonda T,Valle Raleigh J,Mauro AG,Lambert JM,Cowart LA,Salloum FN

Wed May 25 00:00:00 UTC 2022

To Be or Not to Be: The Divergent Action and Metabolism of Sphingosine-1 Phosphate in Pancreatic Beta-Cells in Response to Cytokines and Fatty Acids.

International journal of molecular sciences

Gurgul-Convey E

Mon Jan 31 00:00:00 UTC 2022

Injectable cartilage matrix hydrogel loaded with cartilage endplate stem cells engineered to release exosomes for non-invasive treatment of intervertebral disc degeneration.

Bioactive materials

Luo L,Gong J,Wang Z,Liu Y,Cao J,Qin J,Zuo R,Zhang H,Wang S,Zhao P,Yang D,Zhang M,Wang Y,Zhang J,Zhou Y,Li C,Ni B,Tian Z,Liu M

Thu Sep 01 00:00:00 UTC 2022

Injectable cartilage matrix hydrogel loaded with cartilage endplate stem cells engineered to release exosomes for non-invasive treatment of intervertebral disc degeneration.

Bioactive materials

Luo L,Gong J,Wang Z,Liu Y,Cao J,Qin J,Zuo R,Zhang H,Wang S,Zhao P,Yang D,Zhang M,Wang Y,Zhang J,Zhou Y,Li C,Ni B,Tian Z,Liu M

Thu Sep 01 00:00:00 UTC 2022

Injectable cartilage matrix hydrogel loaded with cartilage endplate stem cells engineered to release exosomes for non-invasive treatment of intervertebral disc degeneration.

Bioactive materials

Luo L,Gong J,Wang Z,Liu Y,Cao J,Qin J,Zuo R,Zhang H,Wang S,Zhao P,Yang D,Zhang M,Wang Y,Zhang J,Zhou Y,Li C,Ni B,Tian Z,Liu M

Thu Sep 01 00:00:00 UTC 2022

Injectable cartilage matrix hydrogel loaded with cartilage endplate stem cells engineered to release exosomes for non-invasive treatment of intervertebral disc degeneration.

Bioactive materials

Luo L,Gong J,Wang Z,Liu Y,Cao J,Qin J,Zuo R,Zhang H,Wang S,Zhao P,Yang D,Zhang M,Wang Y,Zhang J,Zhou Y,Li C,Ni B,Tian Z,Liu M

Thu Sep 01 00:00:00 UTC 2022

Injectable cartilage matrix hydrogel loaded with cartilage endplate stem cells engineered to release exosomes for non-invasive treatment of intervertebral disc degeneration.

Bioactive materials

Luo L,Gong J,Wang Z,Liu Y,Cao J,Qin J,Zuo R,Zhang H,Wang S,Zhao P,Yang D,Zhang M,Wang Y,Zhang J,Zhou Y,Li C,Ni B,Tian Z,Liu M

Thu Sep 01 00:00:00 UTC 2022

Injectable cartilage matrix hydrogel loaded with cartilage endplate stem cells engineered to release exosomes for non-invasive treatment of intervertebral disc degeneration.

Bioactive materials

Luo L,Gong J,Wang Z,Liu Y,Cao J,Qin J,Zuo R,Zhang H,Wang S,Zhao P,Yang D,Zhang M,Wang Y,Zhang J,Zhou Y,Li C,Ni B,Tian Z,Liu M

Thu Sep 01 00:00:00 UTC 2022

The function of Sphingosine-1-phosphate receptor 2 (S1PR2) in maintaining intestinal barrier and inducing ulcerative colitis.

Bioengineered

Chen T,Gu K,Lin R,Liu Y,Shan Y

Sun May 01 00:00:00 UTC 2022

Ablation of sphingosine kinase 2 suppresses fatty liver-associated hepatocellular carcinoma via downregulation of ceramide transfer protein.

Oncogenesis

Liu XT,Chung LH,Liu D,Chen J,Huang Y,Teo JD,Han XD,Zhao Y,Guan FHX,Tran C,Lee JY,Couttas TA,Liu K,McCaughan GW,Gorrell MD,Don AS,Zhang S,Qi Y

Fri Nov 04 00:00:00 UTC 2022

Clonorchis sinensis infection induces hepatobiliary injury via disturbing sphingolipid metabolism and activating sphingosine 1-phosphate receptor 2.

Frontiers in cellular and infection microbiology

Liu JX,Liu M,Yu GZ,Zhao QQ,Wang JL,Sun YH,Koda S,Zhang B,Yu Q,Yan C,Tang RX,Jiang ZH,Zheng KY

Tue Nov 08 00:00:00 UTC 2022

Proteomic-Based Approach Reveals the Involvement of Apolipoprotein A-I in Related Phenotypes of Autism Spectrum Disorder in the BTBR Mouse Model.

International journal of molecular sciences

Li Q,Shi Y,Li X,Yang Y,Zhang X,Xu L,Ma Z,Wang J,Fan L,Wu L

Sun Dec 04 00:00:00 UTC 2022

Sphingosine-1-Phosphate Protects Against the Development of Cardiac Remodeling via Sphingosine Kinase 2 and the S1PR2/ERK Pathway.

Current medical science

Yan H,Zhao H,Yi SW,Zhuang H,Wang DW,Jiang JG,Shen GF

Mon Aug 01 00:00:00 UTC 2022

Aging-dependent mitochondrial dysfunction mediated by ceramide signaling inhibits antitumor T cell response.

Cell reports

Vaena S,Chakraborty P,Lee HG,Janneh AH,Kassir MF,Beeson G,Hedley Z,Yalcinkaya A,Sofi MH,Li H,Husby ML,Stahelin RV,Yu XZ,Mehrotra S,Ogretmen B

Tue May 04 00:00:00 UTC 2021

Analysis of selective target engagement by small-molecule sphingosine kinase inhibitors using the Cellular Thermal Shift Assay (CETSA).

Cancer biology & therapy

Hengst JA,Dick TE,Smith CD,Yun JK

Tue Sep 01 00:00:00 UTC 2020

Inhibition of sphingosine kinase 2 attenuates hypertrophic scar formation via upregulation of Smad7 in human hypertrophic scar fibroblasts.

Molecular medicine reports

Zeng J,Jiang B,Xiao X,Zhang R

Tue Sep 01 00:00:00 UTC 2020

Mechanism of sphingosine 1-phosphate clearance from blood.

The Biochemical journal

Kharel Y,Huang T,Salamon A,Harris TE,Santos WL,Lynch KR

Fri Mar 13 00:00:00 UTC 2020

The lncRNA FEZF1-AS1 Promotes the Progression of Colorectal Cancer Through Regulating OTX1 and Targeting miR-30a-5p.

Oncology research

Li J,Zhao LM,Zhang C,Li M,Gao B,Hu XH,Cao J,Wang GY

Fri Feb 07 00:00:00 UTC 2020

Generation of mice with hepatocyte-specific conditional deletion of sphingosine kinase 1.

Transgenic research

Yu J,Dong J,Chen K,Ding Y,Yang Z,Lan T

Sat Aug 01 00:00:00 UTC 2020

Metabolic Adaptation of Airway Smooth Muscle Cells to an SPHK2 Substrate Precedes Cytostasis.

American journal of respiratory cell and molecular biology

Blais-Lecours P,Laouafa S,Arias-Reyes C,Santos WL,Joseph V,Burgess JK,Halayko AJ,Soliz J,Marsolais D

Wed Jan 01 00:00:00 UTC 2020

Metabolic Adaptation of Airway Smooth Muscle Cells to an SPHK2 Substrate Precedes Cytostasis.

American journal of respiratory cell and molecular biology

Blais-Lecours P,Laouafa S,Arias-Reyes C,Santos WL,Joseph V,Burgess JK,Halayko AJ,Soliz J,Marsolais D

Wed Jan 01 00:00:00 UTC 2020

Cytoplasmic dynein regulates the subcellular localization of sphingosine kinase 2 to elicit tumor-suppressive functions in glioblastoma.

Oncogene