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Invitrogen
SPINLW1 Polyclonal Antibody
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FIGURE: 1 / 1
SPINLW1 Antibody (PA5-96071) in WB
Western blot analysis of SPINLW1 in extracts of various cell lines. Samples were incubated with SPINLW1 Polyclonal antibody (Product # PA5-96071) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Product Details
PA5-96071
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 22-133 of human EPPIN (NP_0651311)
Conjugate
Form
Liquid
Concentration
0.93 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2807873
Product Specific Information
Immunogen sequence: PGLTDWLFPR RCPKIREECE FQERDVCTKD RQCQDNKKCC VFSCGKKCLD LKQDVCEMPK ETGPCLAYFL HWWYDKKDNT CSMFVYGGCQ GNNNNFQSKA NCLNTCKNKR FP; Positive Samples: Mouse testis; Cellular Location: Cell surface
Target Information
Eppin, an epididymal protease inhibitor, belongs to the WFDC family and to the telomeric cluster. The gene encoding the protein is localized to chromosome 20q12-q13 in centromeric and telomeric clusters. It expresses three mRNAs encoding two isoforms of a cystine-rich protein that contains Kunitz-type and WAP-type (four disulfide core) protease inhibitor consensus sequences. The mouse gene lies in a cluster of putative Eppin-like genes on mouse chromosome 2. Following ejaculation, Eppin is bound to semenogelin in seminal plasma and on human spermatozoa. This complex of Eppin and Semenogelin can provide antimicrobial activity for spermatozoa. It can also provide for the preparation and survival of spermatozoa for fertility in the female reproductive tract. Eppin, which is a secreted protein, is expressed in epididymis and testis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cancer/testis antigen 71; cancer/testis antigen 72; CT71; Epididymal protease inhibitor; Eppin; EPPIN1; EPPIN2; EPPIN3; Protease inhibitor WAP7; serine peptidase inhibitor-like, with Kunitz and WAP domains 1 (eppin); Serine protease inhibitor-like with Kunitz and WAP domains 1; serine protease inhibitor-like, with Kunitz and WAP domains 1 (eppin); WAP four-disulfide core domain protein 7; WAP four-disulfide core domain protein 8
Gene Aliases: 1700024E17Rik; CT71; CT72; dJ461P17.2; EPPIN; SPINLW1; WAP7; WFDC7; Wfdc8
UniProt ID: (Human) O95925, (Rat) D4A2Z2, (Mouse) Q9DA01
Entrez Gene ID: (Human) 57119, (Rat) 685161, (Mouse) 75526
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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