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Invitrogen
SPOP Polyclonal Antibody
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FIGURE: 1 / 7
SPOP Antibody (PA5-100014) in ICC/IF
Immunocytochemistry analysis of SPOP in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with SPOP polyclonal antibody (Product # PA5-100014) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-100014
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human SPOP(Accession O43791), corresponding to amino acid residues P151-D201.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2815544
Product Specific Information
Antibody detects endogenous levels of total SPOP.
Target Information
Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination of target proteins, leading most often to their proteasomal degradation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HIB homolog 1; PDX-1 C-terminal-interacting factor 1; Roadkill homolog 1; speckle type POZ protein; Speckle-type POZ protein
Gene Aliases: AI315626; BTBD32; Pcif1; SPOP; TEF2
UniProt ID: (Human) O43791, (Mouse) Q6ZWS8
Entrez Gene ID: (Human) 8405, (Mouse) 20747, (Rat) 287643
negative regulation of transcription from RNA polymerase II promoter
glucose homeostasis
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
proteasome-mediated ubiquitin-dependent protein catabolic process
negative regulation of sequence-specific DNA binding transcription factor activity
positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway
positive regulation of type B pancreatic cell apoptotic process
regulation of proteolysis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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