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          • Primary Antibodies ›
          • SPOP Antibodies

          Invitrogen

          SPOP Polyclonal Antibody

          View all (17) SPOP antibodies

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          Cite SPOP Polyclonal Antibody

          • Antibody Testing Data (2)
          SPOP Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          SPOP Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          SPOP Antibody (PA5-30546) in WB

          Western Blot analysis of SPOP was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a SPOP Polyclonal Antibody (Product # PA5-30546) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          SPOP Antibody in Western Blot (WB)
          SPOP Antibody in Western Blot (WB)

          Product Details

          PA5-30546

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:3,000
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant protein encompassing a sequence within the center region of human SPOP. The exact sequence is proprietary.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          0.1M tris glycine, pH 7, with 20% glycerol

          Contains

          0.01% thimerosal

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2548020

          Product Specific Information

          Recommended positive controls: A549, HCT116.

          Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (95%), Xenopus laevis (97%), Rhesus Monkey (100%), Bovine (100%).

          Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

          Target Information

          Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination of target proteins, leading most often to their proteasomal degradation.

          ⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: HIB homolog 1; Roadkill homolog 1; speckle type POZ protein; Speckle-type POZ protein

          View more View less

          Gene Aliases: BTBD32; SPOP; TEF2

          View more View less

          UniProt ID: (Human) O43791

          View more View less

          Entrez Gene ID: (Human) 8405

          View more View less

          Function(s)
          protein binding ubiquitin protein ligase binding
          Process(es)
          regulation of proteolysis protein ubiquitination involved in ubiquitin-dependent protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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