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Invitrogen
SPOP Recombinant Rabbit Monoclonal Antibody (2N8L8)
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FIGURE: 1 / 1
SPOP Antibody (MA5-37921) in WB
Western blot analysis of SPOP in extracts of PC-3 cells. Samples were incubated with SPOP Monoclonal antibody (Product # MA5-37921) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Product Details
MA5-37921
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
2N8L8
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 250-350 of human SPOP (O43791)
Conjugate
Form
Liquid
Concentration
1.08 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2897841
Product Specific Information
Positive Samples: PC-3
Immunogen sequence: VFKEMMCFIY TGKAPNLDKM ADDLLAAADK YALERLKVMC EDALCSNLSV ENAAEILILA DLHSADQLKT QAVDFINYHA SDVLETSGWK SMVVSHPHLV A
Target Information
Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination of target proteins, leading most often to their proteasomal degradation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HIB homolog 1; Roadkill homolog 1; speckle type POZ protein; Speckle-type POZ protein
Gene Aliases: BTBD32; SPOP; TEF2
UniProt ID: (Human) O43791
Entrez Gene ID: (Human) 8405
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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