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SPTLC2 Antibody (PA5-88231) in WB
Western blot analysis of SPTLC2 in extracts of various cell lines. Samples were incubated with SPTLC2 Polyclonal antibody (Product # PA5-88231) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-88231
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 423-562 of human SPTLC2 (NP_0048541)
Conjugate
Form
Liquid
Concentration
0.88 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2804758
Product Specific Information
Immunogen sequence: MKCIMGQDGT SLGKECVQQL AENTRYFRRR LKEMGFIIYG NEDSPVVPLM LYMPAKIGAF GREMLKRNIG VVVVGFPATP IIESRARFCL SAAHTKEILD TALKEIDEVG DLLQLKYSRH RLVPLLDRPF DETTYEETED; Positive Samples: BxPC-3, U-87MG, 293T, Mouse kidney, Mouse stomach; Cellular Location: Endoplasmic reticulum membrane, Single-pass membrane protein
Target Information
Serine palmitoyltransferase (SPT), which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It converts L-serine and palmitoyl-CoA to 3-oxosphinganine with pyridoxal 5'-phosphate as a cofactor. SPT2 is the long chain base subunit 2 of mammalian serine palmitoyltransferase. SPT2 is catalytically active but needs its related protein SPT1 for its stabilization and anchoring the holoenzyme to the cytosolic face of the endoplasmic reticulum. As in the case with SPT1, mutations in the SPT2 gene can cause hereditary sensory and autonomic neuropathy type I (HSAN-I), resulting from a loss of SPT activity as well as the accumulation of the atypical and neurotoxic sphingoid metabolite 1-deoxy-sphinganine.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: LCB 2; LCB2a; Long chain base biosynthesis protein 2; Long chain base biosynthesis protein 2a; Serine palmitoyltransferase 2; serine palmitoyltransferase, long chain base subunit 2; serine palmitoyltransferase, subunit II; Serine-palmitoyl-CoA transferase 2; SPT 2
Gene Aliases: AI173915; hLCB2a; HSN1C; KIAA0526; LCB2; LCB2A; mKIAA0526; NSAN1C; SPT2; SPTLC2
UniProt ID: (Human) O15270, (Mouse) P97363
Entrez Gene ID: (Human) 9517, (Mouse) 20773
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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