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Invitrogen
SPY Polyclonal Antibody
FIGURE: 1 / 1
SPY Antibody (PA5-144417) in WB
Western blot analysis of SPY in recombinant protein using a dilution of 1:2,000. Samples were incubated with SPY polyclonal antibody (Product # PA5-144417). Secondary: Goat polyclonal to rabbit IgG at 1:50,000 dilution. Predicted band size: 26.8 kDa. Observed band size: 33 kDa.
Product Details
PA5-144417
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bacteria
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Escherichia coli (strain K12) SPY protein (52-147aa)
Conjugate
Form
Liquid
Concentration
3.92 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% Proclin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3091660
Target Information
An ATP-independent periplasmic chaperone, decreases protein aggregation and helps protein refolding. Binds substrate over a large region of its convex inner surface (PubMed:21317898, PubMed:24497545). Substrate protein folds while it is bound to chaperone (PubMed:26619265). Increasing Spy flexibility increases its substrate affinity and overall chaperone activity (shown for 3 different substrates) (PubMed:24497545). Protects proteins in vitro against tannin inactivation; tannins have antimicrobial activity (PubMed:21317898). Overexpression enhances the stability of otherwise unstable periplasmic proteins (PubMed:21317898).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: b1743; JW1732; Periplasmic chaperone Spyspy
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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