Invitrogen

SRC1 Polyclonal Antibody

SRC1 Antibody in Immunocytochemistry (ICC/IF)

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SRC1 Antibody (PA1-840) in ICC/IF

Immunofluorescence analysis of SRC1 was done on 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with SRC1 Rabbit Polyclonal Antibody (Product # PA1-840) at 1 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a ... View More

SRC1 Antibody in Immunohistochemistry (IHC)

SRC1 Antibody in Western Blot, Immunoprecipitation (WB, IP)

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SRC1 Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. A loss of signal was observed for target protein inSRC1 KO cell line compared to control cell line using SRC1 Polyclonal Antibody (Product # PA1-840). {KO}

Product Details

PA1-840

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Frozen) (IHC (F))

1 µg/mL

Immunocytochemistry (ICC/IF)

1-2 µg/mL

Immunoprecipitation (IP)

View 1 publication 1 publication

ChIP assay (ChIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Avian, Human, Mouse

Published species

Amphibian, Avian, Chicken, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues S(8) S D P A N P D S H K R K G S P C(24) of human SRC-1.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2251288

Product Specific Information

PA1-840 detects steroid receptor coactivator-1 (SRC-1) from human, mouse, and japaneese quail tissues and cells.

PA1-840 has been successfully used in Western blot, immunocytochemistry and immunohistochemistry procedures. By Western blot, this antibody detects an ~165 kDa protein representing SRC-1 and a smaller, unknown protein at ~40 kDa from HeLa cell lysate. Immunocytochemical staining of SRC-1 in HeLa cells with PA1-840 results in nuclear staining.

The PA1-840 immunogen is a synthetic peptide corresponding to residues S(8) S D P A N P D S H K R K G S P C(24) of human SRC-1. This immunizing peptide (Cat. # PEP-044) is available for use in neutralization and control experiments.

Target Information

Clone 1135 recognizes SRC-1, steroid-receptor coactivator-1. SRC-1 is a member of a gene family that functions in the full transcriptional activation of the steroid hormone receptor superfamily and contains intrinsic histone acetyltransferase activity. SRC-1 has a molecular weight of 160 kDa and has a basic helix-loop-helix (bHLH)-PAS domain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Direct regulation of genes involved in sperm release by estrogen and androgen through their receptors and coregulators.

The Journal of steroid biochemistry and molecular biology

Kumar A,Dumasia K,Deshpande S,Balasinor NH

PA1840 was used in ChIP assay to examine the presence of a functional estrogen response element upstream of Arpc1b and Evl genes and androgen response element upstream of Picalm, Eea1, and Stx5a genes

Sat Jul 01 00:00:00 UTC 2017

Seasonal and sexual dimorphisms in expression of androgen receptor and its coactivators in brain and peripheral copulatory tissues of the green anole.

General and comparative endocrinology

Kerver HN,Wade J

PA1-840 was used in western blot to study the role of the androgen receptor and its co-activators in regulating the seasonal breeding of the green anole lizzard

Fri Nov 01 00:00:00 UTC 2013

Research resource: identification of novel coregulators specific for thyroid hormone receptor-β2.

Molecular endocrinology (Baltimore, Md.)

Hahm JB,Privalsky ML

PA1-840 was used in Western Blotting to identify a series of previously unrecognized proteins that selectively interact with thyroid hormone receptorΒ2, compared with the more widely expressed TRΒ1.

Wed May 01 00:00:00 UTC 2013

Distribution and sexually dimorphic expression of steroid receptor coactivator-1 (SRC-1) in the zebra finch brain.

General and comparative endocrinology

Duncan KA,Jimenez P,Carruth LL

PA1-840 was used in immunohistochemistry to identify the effect of steroid hormone levels on SRC-1 expression

Sat Jan 15 00:00:00 UTC 2011

The p160 coactivator PAS-B motif stabilizes nuclear receptor binding and contributes to isoform-specific regulation by thyroid hormone receptors.

The Journal of biological chemistry

Privalsky ML,Lee S,Hahm JB,Young BM,Fong RN,Chan IH

PA1-840 was used in immunoprecipitation to investigate the relationship between p160 coactivators and thyroid hormone receptors.

Fri Jul 17 00:00:00 UTC 2009

The p160 coactivator PAS-B motif stabilizes nuclear receptor binding and contributes to isoform-specific regulation by thyroid hormone receptors.

The Journal of biological chemistry

Privalsky ML,Lee S,Hahm JB,Young BM,Fong RN,Chan IH

PA1-840 was used in immunoprecipitation to investigate the relationship between p160 coactivators and thyroid hormone receptors.

Fri Jul 17 00:00:00 UTC 2009

The p160 coactivator PAS-B motif stabilizes nuclear receptor binding and contributes to isoform-specific regulation by thyroid hormone receptors.

The Journal of biological chemistry

Privalsky ML,Lee S,Hahm JB,Young BM,Fong RN,Chan IH

PA1-840 was used in immunoprecipitation to investigate the relationship between p160 coactivators and thyroid hormone receptors.

Fri Jul 17 00:00:00 UTC 2009

Electromagnetic fields alter the expression of estrogen receptor cofactors in breast cancer cells.

Bioelectromagnetics

Girgert R,Gründker C,Emons G,Hanf V

PA1-840 was used in western blot to investigate the effect of electromagnetic fields on expression of estrogen receptor cofactors in MCF-7 cells

Tue Apr 01 00:00:00 UTC 2008

Screening of genetic and expression alterations of SRC1 gene in prostate cancer.

The Prostate

Mäki HE,Waltering KK,Wallén MJ,Martikainen PM,Tammela TL,van Weerden WM,Vessella RL,Visakorpi T

PA1-840 was used in immunohistochemistry to investigate SRC1 mutations in prostate cancer

Fri Sep 15 00:00:00 UTC 2006

Plasticity in the expression of the steroid receptor coactivator 1 in the Japanese quail brain: effect of sex, testosterone, stress and time of the day.

Neuroscience

Charlier TD,Ball GF,Balthazart J

PA1-840 was used in western blot to investigate the regulation of steroid receptor coactivator 1 expression in Japanese quail brain

Fri Jul 21 00:00:00 UTC 2006

Targeting steroid receptor coactivator-1 expression with locked nucleic acids antisense reveals different thresholds for the hormonal regulation of male sexual behavior in relation to aromatase activity and protein expression.

Behavioural brain research

Charlier TD,Harada N,Ball GF,Balthazart J

PA1-840 was used in western blot to investigate the association between male sexual behavior and aromatase activity and protein expression

Mon Sep 25 00:00:00 UTC 2006

Inhibition of steroid receptor coactivator-1 blocks estrogen and androgen action on male sex behavior and associated brain plasticity.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Charlier TD,Ball GF,Balthazart J

PA1-840 was used in western blot to investigate the effect of steroid receptor coactivator-1 on steroid-dependent gene transcription and male sex behavior.

Wed Jan 26 00:00:00 UTC 2005

Expression of nuclear receptor coactivators in androgen-responsive and -unresponsive motoneurons.

Hormones and behavior

O'Bryant EL,Jordan CL

PA1-840 was used in immunohistochemistry to study nuclear receptor coactivators in rat lumbar motoneurons

Sat Jan 01 00:00:00 UTC 2005

Steroid receptor coactivator-1 is not required for androgen-mediated sexual differentiation of spinal motoneurons.

Neuroendocrinology

Monks DA,Xu J,O'Malley BW,Jordan CL

PA1-840 was used in immunohistochemistry to investigate the association between steroid receptor coactivator-1 and a sexually dimorphic neuromuscular system

Tue Jul 01 00:00:00 UTC 2003

Factor recruitment and TIF2/GRIP1 corepressor activity at a collagenase-3 response element that mediates regulation by phorbol esters and hormones.

The EMBO journal

Rogatsky I,Zarember KA,Yamamoto KR

PA1-840 was used in western blot to investigate the role of TIF2/GRIP1 and its associated receptor on specific transcriptional regulation

Thu Nov 01 00:00:00 UTC 2001

Bioinformatics

Protein Aliases: ASV; bHLHe74; c-SRC; Class E basic helix-loop-helix protein 74; Hin-2 protein; mNRC-1; NCoA-1; Nuclear receptor coactivator 1; Nuclear receptor coactivator protein 1; p60-Src; PAX3/NCOA1 fusion protein; Protein Hin-2; Renal carcinoma antigen NY-REN-52; RIP160; SRC-1; SRC-1 (-Q); SRC-a/NCoA-1; Steroid receptor coactivator 1; steroid receptor coactivator-1

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Gene Aliases: bHLHe42; BHLHE74; F-SRC-1; KAT13A; NCOA1; RIP160; SRC-1; SRC1

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UniProt ID: (Human) Q15788, (Mouse) P70365

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Entrez Gene ID: (Human) 8648, (Mouse) 17977

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Function(s)

RNA polymerase II regulatory region DNA binding RNA polymerase II transcription coactivator activity chromatin binding transcription coactivator activity histone acetyltransferase activity protein binding ligand-dependent nuclear receptor binding enzyme binding estrogen receptor binding ligand-dependent nuclear receptor transcription coactivator activity protein complex binding progesterone receptor binding nuclear hormone receptor binding retinoid X receptor binding protein dimerization activity protein N-terminus binding androgen receptor binding DNA binding signal transducer activity transcription factor binding transferase activity transferase activity, transferring acyl groups retinoic acid receptor binding

Process(es)

positive regulation of transcription from RNA polymerase II promoter by galactose regulation of thyroid hormone mediated signaling pathway transcription, DNA-templated lactation male gonad development bile acid and bile salt transport cerebellum development hippocampus development hypothalamus development cerebral cortex development androgen receptor signaling pathway intracellular receptor signaling pathway response to estradiol response to retinoic acid response to progesterone cellular response to hormone stimulus positive regulation of apoptotic process histone H4 acetylation cellular lipid metabolic process estrous cycle positive regulation of neuron differentiation positive regulation of transcription, DNA-templated positive regulation of female receptivity positive regulation of transcription from RNA polymerase II promoter male mating behavior labyrinthine layer morphogenesis cellular response to Thyroglobulin triiodothyronine regulation of cellular response to drug regulation of transcription, DNA-templated signal transduction regulation of nucleic acid-templated transcription regulation of RNA biosynthetic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SRC1 Polyclonal Antibody

SRC1 Antibody (PA1-840) in ICC/IF

Product Details

PA1-840

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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