Proteintech

SRD5A1 Monoclonal Antibody (1D5D8)

SRD5A1 Antibody in Immunocytochemistry (ICC/IF)

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SRD5A1 Antibody (66329-1-IG) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 66329-1-IG (SRD5A1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).

SRD5A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

66329-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1D5D8

Immunogen

SRD5A1 Fusion Protein Ag23351 (1-163 aa encoded by BC007033)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MATATGVAE ERLLAALAYL QCAVGCAVFA RNRQTNSVYG RHALPSHRLR VPARAAWVVQ ELPSLALPLY QYASESAPRL RSAPNCILLA MFLVHYGHRC LIYPFLMRGG KPMPLLACTM AIMFCTCNGY LQSRYLSHCA VYADDWVTDP RFLIGFGLWL TGML (1-163 aa encoded by BC007033)

Target Information

Steroid 5alpha-Reductase is an important enzyme in androgen physiology because it catalyzes the conversion of testosterone into the more potent 5alpha-dihydro-testosterone, which mediates androgen effects on target tissues. The enzyme exists as two isoforms: type 1, which is expressed mainly in the skin; and type 2, which is expressed mainly in the prostate. In cultured human skin cells, 5alpha-Reductase 1 shows heterogeneity of protein, and has different levels of transcriptional and translational expression. 5alpha-Reductase 1 is expressed in all portions of the hair follicle, whereas 5alpha-Reductase 2 is expressed only in mesenchymal portions. In addition, 5alpha-Reductase 1 is mainly expressed in human breast carcinoma and may play a role in the in situ production and actions of the potent androgen 5alpha-dihydrotestosterone, including inhibition of cancer cell proliferation in hormone-dependent human breast carcinoma. The 5alpha-Reductase-3alpha-hydroxysteroid dehydrogenase complex is present in the human brain, suggesting that the complex may be involved in the synthesis of neuroactive steroids or the catabolism of neurotoxic steroids.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The estrous cycle modulates early-life adversity effects on mouse avoidance behavior through progesterone signaling.

Nature communications

Laham BJ,Murthy SS,Hanani M,Clappier M,Boyer S,Vasquez B,Gould E

Wed Dec 07 00:00:00 UTC 2022

Suppression of steroid 5α-reductase type I promotes cellular apoptosis and autophagy via PI3K/Akt/mTOR pathway in multiple myeloma.

Cell death & disease

Dou R,Qian J,Wu W,Zhang Y,Yuan Y,Guo M,Wei R,Yang S,Jurczyszyn A,Janz S,Beksac M,Gu C,Yang Y

Wed Feb 24 00:00:00 UTC 2021

Effects of Curcumin Combined With the 5-alpha Reductase Inhibitor Dutasteride on LNCaP Prostate Cancer Cells.

In vivo (Athens, Greece)

Nakayama A,Ide H,Lu Y,Takei A,Fukuda K,Osaka A,Arai G,Horie S,Okada H,Saito K

Fri Jun 18 00:00:00 UTC 2021

Steroid 5α-Reductase Type I Induces Cell Viability and Migration via Nuclear Factor-κB/Vascular Endothelial Growth Factor Signaling Pathway in Colorectal Cancer.

Frontiers in oncology

Wei R,Zhong S,Qiao L,Guo M,Shao M,Wang S,Jiang B,Yang Y,Gu C

Thu Oct 01 00:00:00 UTC 2020

Steroid 5α-Reductase Type I Induces Cell Viability and Migration via Nuclear Factor-κB/Vascular Endothelial Growth Factor Signaling Pathway in Colorectal Cancer.

Frontiers in oncology

Wei R,Zhong S,Qiao L,Guo M,Shao M,Wang S,Jiang B,Yang Y,Gu C

Thu Oct 01 00:00:00 UTC 2020

Modulation of AKR1C2 by curcumin decreases testosterone production in prostate cancer.

Cancer science

Ide H,Lu Y,Noguchi T,Muto S,Okada H,Kawato S,Horie S

Sun Apr 01 00:00:00 UTC 2018

Expression of steroidogenic enzymes and metabolism of steroids in COS-7 cells known as non-steroidogenic cells.

Scientific reports

Nozaki M,Haraguchi S,Miyazaki T,Shigeta D,Kano N,Lei XF,Kim-Kaneyama JR,Minakata H,Miyazaki A,Tsutsui K

Thu Feb 01 00:00:00 UTC 2018

Bioinformatics

Protein Aliases: 3-oxo-5-alpha-steroid 4-dehydrogenase 1; S5AR 1; SR type 1; Steroid 5-alpha-reductase 1; steroid 5-alpha-reductase type I; steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1)

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Gene Aliases: S5AR 1; SRD5A1

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UniProt ID: (Human) P18405

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Entrez Gene ID: (Human) 6715

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Function(s)

3-oxo-5-alpha-steroid 4-dehydrogenase activity electron carrier activity amide binding cholestenone 5-alpha-reductase activity NADPH binding

Process(es)

urogenital system development liver development steroid biosynthetic process androgen biosynthetic process sex determination male gonad development cellular response to starvation response to muscle activity diterpenoid metabolic process spinal cord development hippocampus development thalamus development hypothalamus development pituitary gland development cerebral cortex development cell differentiation male genitalia development female genitalia development response to follicle-stimulating hormone cellular response to insulin stimulus serotonin metabolic process progesterone metabolic process response to drug circadian sleep/wake cycle, REM sleep response to estrogen oxidation-reduction process bone development response to growth hormone response to fungicide cellular response to cAMP cellular response to growth factor stimulus cellular response to estradiol stimulus cellular response to testosterone stimulus cellular response to dexamethasone stimulus cellular response to epinephrine stimulus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SRD5A1 Monoclonal Antibody (1D5D8)

SRD5A1 Antibody (66329-1-IG) in ICC/IF

Product Details

66329-1-IG

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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