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Invitrogen
SREBP1 Polyclonal Antibody
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Product Details
PA5-120378
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 200-310 of human SREBF1 (NP_004167.3).
Conjugate
Form
Liquid
Concentration
1.43 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2913950
Product Specific Information
Positive test controls include: A-549, MCF7, U-87MG, Mouse liver. The target is usually found in the following locations: COPII-coated vesicle membrane, Cytoplasmic vesicle, Endoplasmic reticulum membrane, Golgi apparatus membrane, Multi-pass membrane protein, Multi-pass membrane protein, Nucleus.
Immunogen sequence: SLHTQVQSVV PQQLLTVTAA PTAAPVTTTV TSQIQQVPVL LQPHFIKADS LLLTAMKTDG ATVKAAGLSP LVSGTTVQTG PLPTLVSGGT ILATVPLVVD AEKLPINRLA A
Target Information
Sterol regulatory element-binding proteins (SREBPs) are transcription factors that are members of the basic helix-loop-helix leucine zipper family of DNA binding proteins. Three isoforms have been identified in mammalian tissues that vary in structure, regulation, and function. SREBP-1a and SREBP-1c (originally cloned as ADD1) are protein products of alternative promoter usage of the SREBP-1 gene. The third isoform is transcribed from a different gene, SREBP-2. SREBPs are present as 120 kDa inactive precursors in the endoplasmic reticulum (ER) membrane. Upon activation, the SREBP protein is translocated to the Golgi and proteolytic cleavage occurs resulting in a mature transcriptionally active 60-78 kDa fragment. In liver and adipose tissues, SREBPs have a significant influence on lipid and cholesterol accumulation by inducing the transcription of genes involved in these processes. While SREBP-1 is thought to be more important in regulating the expression of genes involved in triglyceride synthesis and accumulation, SREBP-2 has been more closely linked to those involved in cholesterol synthesis and accumulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ADD1; Adipocyte determination- and differentiation-dependent factor 1; bHLHd1; Class D basic helix-loop-helix protein 1; nt-SREBP1; SREBP-1; Sterol regulatory element-binding protein 1; Sterol regulatory element-binding transcription factor 1
Gene Aliases: ADD-1; ADD1; BHLHD1; D630008H06; SREBF1; SREBP-1; SREBP-1a; SREBP-1c; SREBP1; SREBP1a; SREBP1c
UniProt ID: (Human) P36956, (Rat) P56720, (Mouse) Q9WTN3
Entrez Gene ID: (Human) 6720, (Rat) 78968, (Mouse) 20787
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
protein kinase binding
protein complex binding
sterol response element binding
protein dimerization activity
sequence-specific DNA binding
transcription regulatory region DNA binding
negative regulation of transcription from RNA polymerase II promoter
regulation of heart rate by chemical signal
regulation of transcription from RNA polymerase II promoter
lipid metabolic process
aging
circadian rhythm
cholesterol metabolic process
insulin receptor signaling pathway
lipid biosynthetic process
cellular response to starvation
response to glucose
positive regulation of triglyceride biosynthetic process
regulation of fatty acid metabolic process
lung development
positive regulation of histone deacetylation
regulation of protein stability
response to food
response to retinoic acid
response to progesterone
response to glucagon
response to drug
mRNA transcription from RNA polymerase II promoter
fat cell differentiation
positive regulation of cholesterol biosynthetic process
positive regulation of transcription from RNA polymerase II promoter
negative regulation of insulin secretion
response to cAMP
cellular response to fatty acid
regulation of mitophagy
regulation of protein targeting to mitochondrion
transcription, DNA-templated
regulation of transcription, DNA-templated
response to organic cyclic compound
response to insulin
cellular response to insulin stimulus
response to lipid
response to peptide hormone
positive regulation of transcription, DNA-templated
regulation of insulin secretion
response to fatty acid
transcription from RNA polymerase II promoter
steroid metabolic process
positive regulation of fatty acid biosynthetic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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