Invitrogen

SRP14 Polyclonal Antibody

FIGURE: 1 / 4

SRP14 Antibody (PA5-27554) in WB

Western blot analysis of SRP14 in wild-type (WT) and SRP14 knockout (KO) 293T cell extracts (30 µg). Samples were incubated with SRP14 polyclonal antibody (Product # PA5-27554) using a dilution of 1:500 followed by HRP-conjugated anti-rabbit IgG. Separated by 15% SDS-PAGE.

Product Image Gallery

SRP14 Antibody — Figure 4. SRP mediated targeting to the ER ensures efficient spicing of XBP1u mRNA. ( A ) HEK 293 cells were transfected with shRNAs against luciferase (control), SRP14, or SRP54. 5 days after transfection, the cells were replated for transfection with XBP1u and treated with 10 mM DTT for the indicated time periods. The Trizol harvested cells were analyzed as in Figure 2D . A phos-tag gel was used for the Ire1alpha immunoblot. p-Ire1alpha and p-PERK indicate the phosphorylated forms of Ire1alpha and PERK, respectively. * denotes a background band. ( B ) HeLa cells were transfected with control siRNA or siRNA targeting the alpha subunit of the SRP receptor (SRalpha) and analyzed as described in Figure 2D . ( C ) HeLa cells were transfected with the indicated siRNA oligos and treated with 10 mM DTT for 2 hr after 96 hr post-transfection. Cells were harvested in SDS sample buffer and analyzed for IB with indicated antibodies. Upon DTT treatment, the ATF6alpha band disappears due to the cleavage of its N-terminal cytosolic domain. Our ATF6alpha antibodies were not suitable for detecting the cleaved N-terminal cytosolic domain (not shown). Note that depletion of Sec61alpha caused significant reduction of the transmembrane proteins PERK and ATF6alpha. DOI: http://dx.doi.org/ {KD}

Product Details

PA5-27554

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human SRP14. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.73 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2545030

Product Specific Information

Recommended positive controls: Raji.

Predicted reactivity: Mouse (91%), Rat (91%), Zebrafish (83%), Xenopus laevis (94%), Dog (94%), Chicken (94%), Rhesus Monkey (100%), Bovine (94%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. SRP9 together with SRP14 and the Alu portion of the SRP RNA, constitutes the elongation arrest domain of SRP. The complex of SRP9 and SRP14 is required for SRP RNA binding.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A functional link between the co-translational protein translocation pathway and the UPR.

eLife

Plumb R,Zhang ZR,Appathurai S,Mariappan M

PA5-27554 was used in western blot to elucidate the mechanism by which Ire1alpha cleaves mRNAs at the ER membrane.

Wed May 20 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: 18 kDa Alu RNA-binding protein; homologous Alu RNA binding protein; Signal recognition particle 14 kDa protein; signal recognition particle 14kDa (homologous Alu RNA binding protein); SRP14

View more View less

Gene Aliases: ALURBP; SRP14

View more View less

UniProt ID: (Human) P37108

View more View less

Entrez Gene ID: (Human) 6727

View more View less

Function(s)

RNA binding protein binding 7S RNA binding endoplasmic reticulum signal peptide binding poly(A) RNA binding

Process(es)

cotranslational protein targeting to membrane SRP-dependent cotranslational protein targeting to membrane response to drug protein targeting to ER

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

SRP14 Polyclonal Antibody

SRP14 Antibody (PA5-27554) in WB

Product Details

PA5-27554

Western Blot (WB)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!