Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 1
SRP14 Antibody (PA5-88866) in WB
Western blot analysis of SRP14 in extracts of various cell lines. Samples were incubated with SRP14 Polyclonal antibody (Product # PA5-88866) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-88866
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-136 of human SRP14 (NP_0031253)
Conjugate
Form
Liquid
Concentration
0.14 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2805182
Product Specific Information
Immunogen sequence: MVLLESEQFL TELTRLFQKC RTSGSVYITL KKYDGRTKPI PKKGTVEGFE PADNKCLLRA TDGKKKISTV VSSKEVNKFQ MAYSNLLRAN MDGLKKRDKK NKTKKTKAAA AAAAAAPAAA ATAPTTAATT AATAAQ; Positive Samples: HeLa, 22RV1, HT-29, HepG2, A-549; Cellular Location: Cytoplasm
Target Information
Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. SRP9 together with SRP14 and the Alu portion of the SRP RNA, constitutes the elongation arrest domain of SRP. The complex of SRP9 and SRP14 is required for SRP RNA binding.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: (homologous Alu RNA binding protein); 18 kDa Alu RNA-binding protein; homologous Alu RNA binding protein; homologous Alu RNA-binding protein; Signal recognition particle 14 kDa protein; signal recognition particle 14kDa (homologous Alu RNA binding protein); SRP14
Gene Aliases: 14kDa; ALURBP; AW536328; SRP14
UniProt ID: (Human) P37108, (Mouse) P16254
Entrez Gene ID: (Human) 6727, (Mouse) 20813, (Rat) 296076
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support