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SRP72 Antibody (PA5-143946) in ICC/IF
Immunocytochemistry analysis of SRP72 in U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in SRP72 Polyclonal antibody (Product # PA5-143946) using a dilution of 5 μg/mL. DyLight®594 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate f... View More
Product Details
PA5-143946
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human SRP72 recombinant protein (Position: I40-Y460).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3075160
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human K562 whole cell, human HL-60 whole cell, rat pancreas tissue, mouse pamcreas tissue. IHC: human breast cancer tissue, human renal cancer tissue, human thyroid cancer tissue, human liver cancer tissue, mouse brain tissue, rat brain tissue. ICC/IF: U20S cell. Flow: U937 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. Binds the 7S RNA only in presence of SRP68. This ribonucleoprotein complex might interact directly with the docking protein in the ER membrane and possibly participate in the elongation arrest function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: epididymis luminal protein 103; signal recognition particle 72 kDa; Signal recognition particle 72 kDa protein; signal recognition particle 72kD; signal recognition particle 72kDa; Signal recognition particle subunit SRP72; SRP72
Gene Aliases: 5730576P14Rik; 72kDa; AI132477; BC019196; BMFF; BMFS1; C77589; HEL103; SRP72
UniProt ID: (Human) O76094
Entrez Gene ID: (Human) 6731, (Rat) 498351, (Mouse) 66661
Performance Guarantee
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