Invitrogen

SRSF1 Polyclonal Antibody

SRSF1 Antibody in Immunocytochemistry (ICC/IF)

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SRSF1 Antibody (PA5-30220) in ICC/IF

Immunocytochemistry (Confocal immunofluorescence) of SRSF1 in paraformaldehyde-fixed HeLa. Samples were incubated with SRSF1 polyclonal antibody (Product # PA5-30220) using a dilution of 1:500 (green). Alpha-tubulin filaments were labeled with alpha Tubulin monoclonal antibody (Product # MA1-25054) (Red) at 1:2,000.

SRSF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

SRSF1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with SRSF1 siRNA and reduction of signal was observed in Western Blot using SRSF1 Polyclonal Antibody (Product # PA5-30220). {KD}

Product Details

PA5-30220

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:10,000

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Immunoprecipitation (IP)

View 1 publication 1 publication

RNA Immunoprecipitation (RIP)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human SF2. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2547694

Product Specific Information

Recommended positive controls: rat brain, HeLa, HepG2, Mouse brain.

Predicted reactivity: Mouse (100%), Rat (100%), Pig (100%), Chicken (98%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

UBE2E2 catalyzes the covalent attachment of ubiquitin to other proteins.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

mRNA recognition and packaging by the human transcription-export complex.

Nature

Pacheco-Fiallos B,Vorländer MK,Riabov-Bassat D,Fin L,O'Reilly FJ,Ayala FI,Schellhaas U,Rappsilber J,Plaschka C

Published figure using SRSF1 polyclonal antibody (Product # PA5-30220) in Western Blot

Sat Apr 01 00:00:00 UTC 2023

circCELF1 Induces the Apoptosis and Autophagy of Astrocytes in Ischemic Stroke via Upregulating NFAT5.

Cerebrovascular diseases (Basel, Switzerland)

Li W,Teng J

PA5-30220 was used in Immunoprecipitation to unveil the role of a novel circRNA hsa_circ_0000304 (termed as circCELF1) and underlying mechanism in ischemic stroke.

Thu Jun 01 00:00:00 UTC 2023

HOTTIP-dependent R-loop formation regulates CTCF boundary activity and TAD integrity in leukemia.

Molecular cell

Luo H,Zhu G,Eshelman MA,Fung TK,Lai Q,Wang F,Zeisig BB,Lesperance J,Ma X,Chen S,Cesari N,Cogle C,Chen B,Xu B,Yang FC,So CWE,Qiu Y,Xu M,Huang S

PA5-30220 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot, ChIP Assay to conclude that HOTTIP-mediated R-loop formation directly reinforces CTCF chromatin boundary activity and TAD integrity to drive oncogene transcription and leukemia development.

Thu Feb 17 00:00:00 UTC 2022

HOTTIP-dependent R-loop formation regulates CTCF boundary activity and TAD integrity in leukemia.

Molecular cell

Luo H,Zhu G,Eshelman MA,Fung TK,Lai Q,Wang F,Zeisig BB,Lesperance J,Ma X,Chen S,Cesari N,Cogle C,Chen B,Xu B,Yang FC,So CWE,Qiu Y,Xu M,Huang S

Thu Feb 17 00:00:00 UTC 2022

An IGF-1R-mTORC1-SRPK2 signaling Axis contributes to FASN regulation in breast cancer.

BMC cancer

McClellan B,Gries P,Harlow B,Tiziani S,Jolly C,deGraffenried L

PA5-30220 was used in RNA Immunoprecipitation to establish a potential IGF-1-mTORC1-SRPK2-FASN axis in breast cancer, which could be a potential therapeutic target for cancers that overexpress FASN and components of the IGF-1R pathway.

Mon Sep 12 00:00:00 UTC 2022

Elucidating the Antiviral Mechanism of Different MARCH Factors.

mBio

Umthong S,Lynch B,Timilsina U,Waxman B,Ivey EB,Stavrou S

PA5-30220 was used in Western Blot to perform a comprehensive analysis on a number of envelope glycoproteins and show that MARCH proteins have broad antiviral functions.

Tue Mar 02 00:00:00 UTC 2021

Moringa oleifera Gold Nanoparticles Modulate Oncogenes, Tumor Suppressor Genes, and Caspase-9 Splice Variants in A549 Cells.

Journal of cellular biochemistry

Tiloke C,Phulukdaree A,Anand K,Gengan RM,Chuturgoon AA

Published figure using SRSF1 polyclonal antibody (Product # PA5-30220) in Western Blot

Sat Oct 01 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: Alternative-splicing factor 1; ASF-1; ASF/SF2; pre-mRNA-splicing factor SF2, P33 subunit; Pre-mRNA-splicing factor SRp30a; Serine/arginine-rich splicing factor 1; splicing factor 2; Splicing factor, arginine/serine-rich 1; splicing factor, arginine/serine-rich 1 (ASF/SF2); splicing factor, arginine/serine-rich 1B; splicing factor, arginine/serine-rich, 30-KD, A; SR splicing factor 1

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Gene Aliases: 1110054N12Rik; 5730507C05Rik; 6330415C05Rik; AI482334; ASF; AW491331; OK/SW-cl.3; SF2; SF2P33; SFRS1; SRp30a; SRSF1

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UniProt ID: (Human) Q07955, (Mouse) Q6PDM2

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Entrez Gene ID: (Human) 6426, (Rat) 689890, (Mouse) 110809

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Function(s)

nucleotide binding RNA binding mRNA binding protein binding poly(A) RNA binding RS domain binding nucleic acid binding

Process(es)

mRNA 5'-splice site recognition in utero embryonic development regulation of transcription, DNA-templated regulation of translation RNA splicing regulation of mRNA stability regulation of mRNA splicing, via spliceosome cardiac muscle contraction mRNA processing transport mRNA transport alternative mRNA splicing, via spliceosome mRNA splicing, via spliceosome termination of RNA polymerase II transcription mRNA splice site selection RNA export from nucleus mRNA export from nucleus mRNA 3'-end processing

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SRSF1 Polyclonal Antibody

SRSF1 Antibody (PA5-30220) in ICC/IF

Product Details

PA5-30220

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

RNA Immunoprecipitation (RIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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