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Invitrogen
SRSF1 Polyclonal Antibody
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SRSF1 Antibody (PA5-80069) in IHC (P)
Immunohistochemical analysis of SF2/SRSF1 in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution).The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/mL rabbit anti-SF2/SRSF1 antibody (Product # PA5-80069) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit ... View More
Product Details
PA5-80069
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of Human SF2 (6-33aa VIRGPAGNNDCRIYVGNLPPDIRTKDIE).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2747184
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human SW620 whole cell, human MCF-7 whole cell, human 293T whole cell, rat brain tissue, rat liver tissue, mouse brain tissue, mouse liver tissue. IHC: human placenta tissue, mouse brain tissue, rat brain tissue.
Target Information
UBE2E2 catalyzes the covalent attachment of ubiquitin to other proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Alternative-splicing factor 1; ASF-1; ASF/SF2; pre-mRNA-splicing factor SF2, P33 subunit; Pre-mRNA-splicing factor SRp30a; Serine/arginine-rich splicing factor 1; splicing factor 2; Splicing factor, arginine/serine-rich 1; splicing factor, arginine/serine-rich 1 (ASF/SF2); splicing factor, arginine/serine-rich 1B; splicing factor, arginine/serine-rich, 30-KD, A; SR splicing factor 1
Gene Aliases: 1110054N12Rik; 5730507C05Rik; 6330415C05Rik; AI482334; ASF; AW491331; OK/SW-cl.3; SF2; SF2P33; SFRS1; SRp30a; SRSF1
UniProt ID: (Human) Q07955, (Mouse) Q6PDM2
Entrez Gene ID: (Human) 6426, (Rat) 689890, (Mouse) 110809
mRNA 5'-splice site recognition
in utero embryonic development
regulation of transcription, DNA-templated
regulation of translation
RNA splicing
regulation of mRNA stability
regulation of mRNA splicing, via spliceosome
cardiac muscle contraction
mRNA processing
transport
mRNA transport
alternative mRNA splicing, via spliceosome
mRNA splicing, via spliceosome
termination of RNA polymerase II transcription
mRNA splice site selection
RNA export from nucleus
mRNA export from nucleus
mRNA 3'-end processing
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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