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Invitrogen
SRSF2 Polyclonal Antibody
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FIGURE: 1 / 8
SRSF2 Antibody (PA5-115868) in ICC/IF
Immunocytochemistry analysis of SRSF2 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115868) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Product Details
PA5-115868
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human SRSF2(Accession Q01130), corresponding to amino acid residues D73-R123.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2900502
Product Specific Information
Antibody detects endogenous levels of total SC35.
Target Information
The protein encoded by SC35 is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Two transcript variants encoding the same protein and one non-coding transcript variant have been found for SC35. In addition, a pseudogene of SC35 has been found on chromosome 11.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MRF-1; myelin regulatory factor 1; Protein PR264; Putative myelin regulatory factor 1; SC-35; Serine/arginine-rich splicing factor 2; Splicing component, 35 kDa; Splicing factor SC35; Splicing factor, arginine/serine-rich 2; splicing factor, arginine/serine-rich 2 (SC-35); SR splicing factor 2
Gene Aliases: D11Wsu175e; MRF-1; PR264; SC-35; SC35; Sfrs10; SFRS2; SFRS2A; SRp30b; SRSF2
UniProt ID: (Human) Q01130, (Mouse) Q62093, (Rat) Q6PDU1
Entrez Gene ID: (Human) 6427, (Mouse) 20382, (Rat) 494445
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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