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Invitrogen
SRSF3 Polyclonal Antibody
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FIGURE: 1 / 3
SRSF3 Antibody (PA5-80070) in ICC/IF
Immunocytochemistry analysis of SRSF3 in A549 cells. Samples were incubated with SRSF3 polyclonal antibody (Product # PA5-80070) using a dilution of 5 μg/mL overnight at 4°C followed by DyLight®488 Conjugated Goat Anti-Rabbit IgG and DyLight®550 Conjugated Goat Anti-Mouse IgG for 30 minutes at 37°C. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples incubated with primary and mouse anti-Beta Tubulin antibody. Visualize using a fluorescence micro... View More
Product Details
PA5-80070
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human SFRS3 (1-29aa MHRDSCPLDCKVYVGNLGNNGNKTELERA).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.01mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2747185
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human SH-SY5Y whole cell, human 293T whole cell, human HepG2 whole cell, human SiHa whole cell. ICC/IF: A549 cell. Flow: SH-SY5Y cells.
Target Information
SR proteins appear to be recruited from nuclear "speckles", in which they are concentrated, to sites of transcription in order to spatially coordinate transcription and pre-mRNA splicing within the cell nucleus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: pre-mRNA splicing factor SRp20; Pre-mRNA-splicing factor SRP20; Serine/arginine-rich splicing factor 3; Splicing factor, arginine/serine-rich 3; splicing factor, arginine/serine-rich, 20-kD
Gene Aliases: SFRS3; SRP20; SRSF3
UniProt ID: (Human) P84103
Entrez Gene ID: (Human) 6428
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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