Invitrogen

SRSF3 Monoclonal Antibody (1H4G7)

FIGURE: 1 / 11

SRSF3 Antibody (33-9400) in WB

Western blot of HeLa cell lysates using Mouse anti-SR Proteins monoclonal Antibody (Product # 33-9400).

Product Image Gallery

SRSF3 Antibody — Figure 6 Digoxin alters the activity of SR protein kinases and induces modification of a subset of SR proteins. (A) To assess the effect of digoxin on CLK kinase function, HeLa rtTA-HIV-Delta Mls cells were transfected with vectors expressing GFP-tagged CLK1. Twenty-four hours post-transfection, cells were treated with DMSO or 100 nM digoxin. After 24 h of treatment, cells were fixed, stained for SC35/SRSF2 nuclear speckles (Texas Red), and nuclei stained with DAPI. Images are representative of the localization patterns observed from >=5 independent experiments. Magnification 630x. (B, C, D) Western blot analysis of the effect of digoxin treatment on SR proteins. Lysates were harvested from HeLa rtTA-HIV-Delta Mls cells treated with 100 nM digoxin or DMSO and were induced with Dox (+) or left uninduced (-) as described in Fig. 1 . Immunoblots were probed by specific antibody for (B) phospho-SR proteins (1H4) or (C) SRp20, Tra2beta1, 9G8, or SF2/ASF (SF2). (D) Cell lysates from above were dephosphorylated by treatment with (+) and without (-) calf intestinal alkaline phosphatase (CIP), then immunoblotted with a SRp20-specific antibody. Arrows on the right indicate the protein specie(s) detected and their modified/unmodified isoform(s). Anti-tubulin (Anti-Tub.) blots for each experiment served as an internal loading control for the relative amount of protein lysate per lane. Immunoblots are representative of >=4 independent trials. {TM}

Product Details

33-9400

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 9 publications 9 publications

Immunocytochemistry (ICC/IF)

Assay-dependent

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

View 1 publication 1 publication

ChIP assay (ChIP)

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Xenopus

Published species

Hamster, Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

1H4G7

Immunogen

Mass-isolated nuclei from Xenopus Laevis oocytes.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533150

Target Information

SR proteins appear to be recruited from nuclear "speckles", in which they are concentrated, to sites of transcription in order to spatially coordinate transcription and pre-mRNA splicing within the cell nucleus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Therapeutic manipulation of IKBKAP mis-splicing with a small molecule to cure familial dysautonomia.

Nature communications

Ajiro M,Awaya T,Kim YJ,Iida K,Denawa M,Tanaka N,Kurosawa R,Matsushima S,Shibata S,Sakamoto T,Studer L,Krainer AR,Hagiwara M

33-9400 was used in Western Blot to indicate that small synthetic molecules affecting phosphorylation state of SRSFs is available as a new therapeutic modality for mechanism-oriented precision medicine of splicing diseases.

Fri Jul 23 00:00:00 UTC 2021

Splicing Enhancers at Intron-Exon Borders Participate in Acceptor Splice Sites Recognition.

International journal of molecular sciences

Kováčová T,Souček P,Hujová P,Freiberger T,Grodecká L

33-9400 was used in Western Blotting to analyse 3'splice site recognition where the intron-exon border is bound by a ubiquitous splicing regulator SRSF1.

Tue Sep 08 00:00:00 UTC 2020

Splicing Enhancers at Intron-Exon Borders Participate in Acceptor Splice Sites Recognition.

International journal of molecular sciences

Kováčová T,Souček P,Hujová P,Freiberger T,Grodecká L

33-9400 was used in Western Blotting to analyse 3'splice site recognition where the intron-exon border is bound by a ubiquitous splicing regulator SRSF1.

Tue Sep 08 00:00:00 UTC 2020

Anti-tumor efficacy of a novel CLK inhibitor via targeting RNA splicing and MYC-dependent vulnerability.

EMBO molecular medicine

Iwai K,Yaguchi M,Nishimura K,Yamamoto Y,Tamura T,Nakata D,Dairiki R,Kawakita Y,Mizojiri R,Ito Y,Asano M,Maezaki H,Nakayama Y,Kaishima M,Hayashi K,Teratani M,Miyakawa S,Iwatani M,Miyamoto M,Klein MG,Lane W,Snell G,Tjhen R,He X,Pulukuri S,Nomura T

33-9400 was used in Western Blotting to identify that T-025 functions as an orally available and potent inhibitor of Cdc2-like kinases, evolutionally conserved kinases that facilitate exon recognition in the splicing machinery.

Fri Jun 01 00:00:00 UTC 2018

Balanced splicing at the Tat-specific HIV-1 3'ss A3 is critical for HIV-1 replication.

Retrovirology

Erkelenz S,Hillebrand F,Widera M,Theiss S,Fayyaz A,Degrandi D,Pfeffer K,Schaal H

33-9400 was used in western blot to investigate viral tat-mRNA splicing.

Sat Mar 28 00:00:00 UTC 2015

Digoxin suppresses HIV-1 replication by altering viral RNA processing.

PLoS pathogens

Wong RW,Balachandran A,Ostrowski MA,Cochrane A

33-9400 was used in western blot to examine the effects of RNA splicing modulators on viral RNA metabolism

Fri Mar 01 00:00:00 UTC 2013

Serine arginine splicing factor 3 is involved in enhanced splicing of glucose-6-phosphate dehydrogenase RNA in response to nutrients and hormones in liver.

The Journal of biological chemistry

Walsh CM,Suchanek AL,Cyphert TJ,Kohan AB,Szeszel-Fedorowicz W,Salati LM

33-9400 was used in ChIP assay and western blot to report that nutrients and hormones control the activity of serine-arginine-rich proteins which regulate the splicing of G6PD mRNA.

Fri Jan 25 00:00:00 UTC 2013

Serine arginine splicing factor 3 is involved in enhanced splicing of glucose-6-phosphate dehydrogenase RNA in response to nutrients and hormones in liver.

The Journal of biological chemistry

Walsh CM,Suchanek AL,Cyphert TJ,Kohan AB,Szeszel-Fedorowicz W,Salati LM

33-9400 was used in ChIP assay and western blot to report that nutrients and hormones control the activity of serine-arginine-rich proteins which regulate the splicing of G6PD mRNA.

Fri Jan 25 00:00:00 UTC 2013

Serine arginine splicing factor 3 is involved in enhanced splicing of glucose-6-phosphate dehydrogenase RNA in response to nutrients and hormones in liver.

The Journal of biological chemistry

Walsh CM,Suchanek AL,Cyphert TJ,Kohan AB,Szeszel-Fedorowicz W,Salati LM

33-9400 was used in ChIP assay and western blot to report that nutrients and hormones control the activity of serine-arginine-rich proteins which regulate the splicing of G6PD mRNA.

Fri Jan 25 00:00:00 UTC 2013

The cardiotonic steroid digitoxin regulates alternative splicing through depletion of the splicing factors SRSF3 and TRA2B.

RNA (New York, N.Y.)

Anderson ES,Lin CH,Xiao X,Stoilov P,Burge CB,Black DL

Tue May 01 00:00:00 UTC 2012

Engineering DYRK1A overdosage yields Down syndrome-characteristic cortical splicing aberrations.

Neurobiology of disease

Toiber D,Azkona G,Ben-Ari S,Torán N,Soreq H,Dierssen M

33-9400 was used in immunohistochemistry and western blot to assess the splicing machinery in Down syndrome brains

Fri Oct 01 00:00:00 UTC 2010

Engineering DYRK1A overdosage yields Down syndrome-characteristic cortical splicing aberrations.

Neurobiology of disease

Toiber D,Azkona G,Ben-Ari S,Torán N,Soreq H,Dierssen M

33-9400 was used in immunohistochemistry and western blot to assess the splicing machinery in Down syndrome brains

Fri Oct 01 00:00:00 UTC 2010

Changes in brain MicroRNAs contribute to cholinergic stress reactions.

Journal of molecular neuroscience : MN

Meerson A,Cacheaux L,Goosens KA,Sapolsky RM,Soreq H,Kaufer D

33-9400 was used in western blot to study how stress-regulated miRs regulate alternative splicing

Fri Jan 01 00:00:00 UTC 2010

RNA splicing capability of live neuronal dendrites.

Proceedings of the National Academy of Sciences of the United States of America

Glanzer J,Miyashiro KY,Sul JY,Barrett L,Belt B,Haydon P,Eberwine J

Tue Nov 15 00:00:00 UTC 2005

RNA folding affects the recruitment of SR proteins by mouse and human polypurinic enhancer elements in the fibronectin EDA exon.

Molecular and cellular biology

Buratti E,Muro AF,Giombi M,Gherbassi D,Iaconcig A,Baralle FE

Sun Feb 01 00:00:00 UTC 2004

RNA folding affects the recruitment of SR proteins by mouse and human polypurinic enhancer elements in the fibronectin EDA exon.

Molecular and cellular biology

Buratti E,Muro AF,Giombi M,Gherbassi D,Iaconcig A,Baralle FE

Sun Feb 01 00:00:00 UTC 2004

Cyclin L is an RS domain protein involved in pre-mRNA splicing.

The Journal of biological chemistry

Dickinson LA,Edgar AJ,Ehley J,Gottesfeld JM

33-9400 was used in Western Blot to report the cDNA cloning and functional characterisation of human cyclin L, a novel cyclin related to the C-type cyclines that are involved in regulation of RNA polymerase II transcription.

Fri Jul 12 00:00:00 UTC 2002

Bioinformatics

Protein Aliases: pre-mRNA splicing factor SRp20; Pre-mRNA-splicing factor SRP20; Serine/arginine-rich splicing factor 3; Splicing factor, arginine/serine-rich 3; splicing factor, arginine/serine-rich, 20-kD

View more View less

Gene Aliases: SFRS3; SRP20; SRSF3

View more View less

UniProt ID: (Human) P84103

View more View less

Entrez Gene ID: (Human) 6428

View more View less

Function(s)

nucleotide binding RNA binding protein binding phospholipase binding poly(A) RNA binding sequence-specific mRNA binding

Process(es)

mRNA splicing, via spliceosome termination of RNA polymerase II transcription RNA export from nucleus mRNA export from nucleus mRNA 3'-end processing regulation of mRNA splicing, via spliceosome

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

SRSF3 Monoclonal Antibody (1H4G7)

SRSF3 Antibody (33-9400) in WB

Product Details

33-9400

Western Blot (WB)

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!