Invitrogen

SRSF6 Polyclonal Antibody

SRSF6 Antibody in Immunocytochemistry (ICC/IF)

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SRSF6 Antibody (PA5-41810) in ICC/IF

Immunofluorescence analysis of SRSF6 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with SRSF6 Polyclonal Antibody (Product # PA5-41810) at 5 µg/mL 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) for ... View More

SRSF6 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

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SRSF6 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. T-47D cells were transfected with SRSF6 siRNA and reduction of signal was observed in Western Blot using SRSF6 Polyclonal Antibody (Product # PA5-41810). {KD}

Product Details

PA5-41810

Applications

Tested Dilution

Publications

Western Blot (WB)

0.2-1 µg/mL

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

5 µg/mL

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide directed towards the middle region of human SFRS6

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS with 2% sucrose

Contains

0.09% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2607949

Product Specific Information

Peptide sequence: KERTNEGVIE FRSYSDMKRA LDKLDGTEIN GRNIRLIEDK PRTSHRRSYS

Sequence homology: Cow: 100%; Dog: 100%; Guinea Pig: 82%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 85%

Target Information

SFRS6 is involved in mRNA splicing and may play a role in the determination of alternative splicing. It belongs to the splicing factor SR family and has been shown to bind with and modulate another member of the family, SFRS12. The protein encoded by this gene is involved in mRNA splicing and may play a role in the determination of alternative splicing. The encoded nuclear protein belongs to the splicing factor SR family and has been shown to bind with and modulate another member of the family, SFRS12.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Splicing factor SRSF6 mediates pleural fibrosis.

JCI insight

Liang LM,Xiong L,Cheng PP,Chen SJ,Feng X,Zhou YY,Niu Q,Wang M,Chen Q,Song LJ,Yu F,He XL,Xiang F,Wang X,Ye H,Ma WL

PA5-41810 was used in Immunohistochemistry (Paraffin) to find the role of the SRSF6 protein, which belong to a family of highly conserved RNA-binding splicing-factors, in the pleural mesothelial cells of pleural fibrosis.

Mon May 24 00:00:00 UTC 2021

Splicing factor SRSF6 mediates pleural fibrosis.

JCI insight

Liang LM,Xiong L,Cheng PP,Chen SJ,Feng X,Zhou YY,Niu Q,Wang M,Chen Q,Song LJ,Yu F,He XL,Xiang F,Wang X,Ye H,Ma WL

Mon May 24 00:00:00 UTC 2021

Splicing factor SRSF6 mediates pleural fibrosis.

JCI insight

Liang LM,Xiong L,Cheng PP,Chen SJ,Feng X,Zhou YY,Niu Q,Wang M,Chen Q,Song LJ,Yu F,He XL,Xiang F,Wang X,Ye H,Ma WL

Mon May 24 00:00:00 UTC 2021

The TIA1 RNA-Binding Protein Family Regulates EIF2AK2-Mediated Stress Response and Cell Cycle Progression.

Molecular cell

Meyer C,Garzia A,Mazzola M,Gerstberger S,Molina H,Tuschl T

PA5-41810 was used in Western Blotting to study the role of TIA1 protein family in the mediated stress response and cell cycle progression.

Thu Feb 15 00:00:00 UTC 2018

The TIA1 RNA-Binding Protein Family Regulates EIF2AK2-Mediated Stress Response and Cell Cycle Progression.

Molecular cell

Meyer C,Garzia A,Mazzola M,Gerstberger S,Molina H,Tuschl T

PA5-41810 was used in Western Blotting to study the role of TIA1 protein family in the mediated stress response and cell cycle progression.

Thu Feb 15 00:00:00 UTC 2018

Bioinformatics

Protein Aliases: epididymis secretory protein Li 91; MGC5045; Pre-mRNA-splicing factor SRP55; Serine/arginine-rich splicing factor 6; Splicing factor, arginine/serine-rich 6; splicing factor, arginine/serine-rich, 55 kDa; SR splicing factor 6

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Gene Aliases: B52; HEL-S-91; SFRS6; SRP55; SRSF6

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UniProt ID: (Human) Q13247

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Entrez Gene ID: (Human) 6431

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Function(s)

nucleotide binding RNA binding protein binding pre-mRNA binding poly(A) RNA binding

Process(es)

alternative mRNA splicing, via spliceosome regulation of alternative mRNA splicing, via spliceosome mRNA splicing, via spliceosome termination of RNA polymerase II transcription mRNA splice site selection RNA export from nucleus mRNA export from nucleus regulation of keratinocyte proliferation mRNA 3'-end processing negative regulation of keratinocyte differentiation negative regulation of mRNA splicing, via spliceosome positive regulation of epithelial cell proliferation involved in lung morphogenesis negative regulation of cell death regulation of wound healing

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SRSF6 Polyclonal Antibody

SRSF6 Antibody (PA5-41810) in ICC/IF

Product Details

PA5-41810

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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