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SSEA1 Antibody (MA1-022-PE) in Flow
Flow cytometry analysis of SSEA-1 in F9 (red filled histogram) and NCCIT cells (red unfilled histogram) compared to unstained cells (blue histogram). Positive staining is observed on F9 cells when compared to no antibody control (middle panel) and to SSEA-1-negative undifferentiated NCCIT cells (right panel). Cells were fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with 2% BSA-PBS for 30 minutes at room temperature, and incubated with a PE-conjugated SSEA-1 monoclonal antibody (Product # MA1-022-PE) in 2% BSA-PBS a... View More
Product Details
MA1-022-PE
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgM
Class
Monoclonal
Type
Antibody
Clone
MC-480
Immunogen
BALB/c mouse immunized with F9 teratocarcinoma stem cells (X-irradiated).
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography - MBP
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2536697
Product Specific Information
MA1-022-PE has been successfully used in ICC/IF and flow cytometry applications on mouse and human samples.
Target Information
CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin's disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 3-fucosyl-N-acetyl-lactosamine epitope; 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase; alpha (1,3) fucosyltransferase, myeloid specific; alpha (1,3) fucosyltransferase, myeloid-specific; alpha 1,3-fucosyltransferase; Alpha-(1,3)-fucosyltransferase 4; ELAM ligand fucosyltransferase; ELAM-1 ligand fucosyltransferase; Fuc-TIV; Fucosyltransferase 4; fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific); Fucosyltransferase IV; fucT-IV; FUTIV; Galactoside 3-L-fucosyltransferase; Lewis X; SSEA-1; stage-specific embryonic antigen 1
Gene Aliases: AI451562; CD15; ELFT; FAL; FCT3A; FUC-TIV; FucT-IV; FUT4; FUTIV; LeX; SSEA-1; Ssea1
UniProt ID: (Human) P22083, (Mouse) Q11127
Entrez Gene ID: (Human) 2526, (Mouse) 14345
fucosyltransferase activity
alpha-(1->3)-fucosyltransferase activity
alpha-1,6-mannosyltransferase activity
alpha-1,2-mannosyltransferase activity
mannosyltransferase activity
alpha-1,3-mannosyltransferase activity
alpha-1,3-galactosyltransferase activity
UDP-glucose:glycoprotein glucosyltransferase activity
glycolipid mannosyltransferase activity
oligosaccharyl transferase activity
dolichyl-phosphate-glucose-glycolipid alpha-glucosyltransferase activity
acetylglucosaminyltransferase activity
acetylgalactosaminyltransferase activity
galactosyltransferase activity
O antigen polymerase activity
lipopolysaccharide-1,6-galactosyltransferase activity
transferase activity
transferase activity, transferring glycosyl groups
cellulose synthase activity
9-phenanthrol UDP-glucuronosyltransferase activity
1-phenanthrol glycosyltransferase activity
9-phenanthrol glycosyltransferase activity
1,2-dihydroxy-phenanthrene glycosyltransferase activity
phenanthrol glycosyltransferase activity
beta-1,4-mannosyltransferase activity
alpha-1,2-galactosyltransferase activity
dolichyl pyrophosphate Man7GlcNAc2 alpha-1,3-glucosyltransferase activity
lipopolysaccharide-1,5-galactosyltransferase activity
dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase activity
dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase activity
inositol phosphoceramide synthase activity
glucosyltransferase activity
alpha-(1->6)-fucosyltransferase activity
indole-3-butyrate beta-glucosyltransferase activity
salicylic acid glucosyltransferase (ester-forming) activity
salicylic acid glucosyltransferase (glucoside-forming) activity
benzoic acid glucosyltransferase activity
chondroitin hydrolase activity
dolichyl-pyrophosphate Man7GlcNAc2 alpha-1,6-mannosyltransferase activity
cytokinin 9-beta-glucosyltransferase activity
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