Invitrogen

SSEA1 Monoclonal Antibody (eBioMC-480 (MC-480)), PE, eBioscience™

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SSEA1 Antibody (12-8813-42) in Flow

THP-1 and Ramos cells were fixed and permeabilized using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-000) and then stained with SSEA1 Monoclonal Antibody (eBioMC-480 (MC-480), PE, eBioscience™ (Product # 12-8813-42) at a concentration of 0.25 µg (yellow histogram) and CD19 Monoclonal Antibody (HIB19), APC, eBioscience™ (Product # 17-0199-42) (red histogram) at a concentration of 1 µg or left unstained (black histogram). CD15 and CD19 were mutually exclusively present on THP-1 and Ramos cells respectively. Vi... View More

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SSEA1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of CD15 was observed in THP-1 in comparison to low to negative in Ramos using SSEA1 Monoclonal Antibody (eBioMC-480 (MC-480), PE, eBioscience™ (Product # 12-8813-42). {RE}

Product Details

12-8813-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 19 publications 19 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

eBioMC-480 (MC-480)

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10852715

Product Specific Information

Description: The eBioMC-480 (MC-480) antibody reacts with the stage-specific embryonic antigen-1 (SSEA-1), a carbohydrate epitope expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine and human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Differentiation of human EC results in an increase in SSEA-1 expression, while in the mouse expression is diminished. Expression of the carbohydrate moiety is also found on mature human granulocytes (on CD15) and some monocytes. SSEA-1 is associated with cell adhesion, migration and differentiation.

Applications Reported: This eBioMC-480 (MC-480) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioMC-480 (MC-480) antibody has been pre-titrated and tested by flow cytometric analysis of the F9 cell line. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin's disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

High-throughput screening in postimplantation haploid epiblast stem cells reveals Hs3st3b1 as a modulator for reprogramming.

Stem cells translational medicine

Gao Q,Zhang W,Zhao Y,Tian Y,Wang Y,Zhang J,Geng M,Xu M,Yao C,Wang H,Li L,Liu Y,Shuai L

Published figure using SSEA1 monoclonal antibody (Product # 12-8813-42) in Flow Cytometry

Sat May 01 00:00:00 UTC 2021

ECE2 regulates neurogenesis and neuronal migration during human cortical development.

EMBO reports

Buchsbaum IY,Kielkowski P,Giorgio G,O'Neill AC,Di Giaimo R,Kyrousi C,Khattak S,Sieber SA,Robertson SP,Cappello S

Published figure using SSEA1 monoclonal antibody (Product # 12-8813-42) in Flow Cytometry

Wed May 06 00:00:00 UTC 2020

Induced pluripotent stem cells reprogrammed from primary dendritic cells provide an abundant source of immunostimulatory dendritic cells for use in immunotherapy.

Stem cells (Dayton, Ohio)

Horton C,Davies TJ,Lahiri P,Sachamitr P,Fairchild PJ

12-8813 was used in Flow cytometry/Cell sorting to investigate if epigenetic memory of iPSC-derived dendritic cells may be productively exploited for the generation of potently immunogenic dendritic cells for immunotherapeutic applications.

Wed Jan 01 00:00:00 UTC 2020

STAT3 Inhibition Combined with CpG Immunostimulation Activates Antitumor Immunity to Eradicate Genetically Distinct Castration-Resistant Prostate Cancers.

Clinical cancer research : an official journal of the American Association for Cancer Research

Moreira D,Adamus T,Zhao X,Su YL,Zhang Z,White SV,Swiderski P,Lu X,DePinho RA,Pal SK,Kortylewski M

12-8813 was used in Flow cytometry/Cell sorting to show a novel strategy combining STAT3 inhibition with Toll-like Receptor 9 (TLR9) stimulation to unleash immune response against prostate cancers regardless of the genetic background.

Sat Dec 01 00:00:00 UTC 2018

Comparative glycoproteomics of stem cells identifies new players in ricin toxicity.

Nature

Stadlmann J,Taubenschmid J,Wenzel D,Gattinger A,Dürnberger G,Dusberger F,Elling U,Mach L,Mechtler K,Penninger JM

12-8813 was used in Flow cytometry/Cell sorting to develop a comparative glycoproteomics platform for studying genome-wide protein glycosylation and glycan modifications in complex biological systems.

Thu Sep 28 00:00:00 UTC 2017

A vital sugar code for ricin toxicity.

Cell research

Taubenschmid J,Stadlmann J,Jost M,Klokk TI,Rillahan CD,Leibbrandt A,Mechtler K,Paulson JC,Jude J,Zuber J,Sandvig K,Elling U,Marquardt T,Thiel C,Koerner C,Penninger JM

Published figure using SSEA1 monoclonal antibody (Product # 12-8813-42) in Flow Cytometry

Wed Nov 01 00:00:00 UTC 2017

Noncanonical function of DGCR8 controls mESC exit from pluripotency.

The Journal of cell biology

Cirera-Salinas D,Yu J,Bodak M,Ngondo RP,Herbert KM,Ciaudo C

Published figure using SSEA1 monoclonal antibody (Product # 12-8813-42) in Flow Cytometry

Wed Feb 01 00:00:00 UTC 2017

Drug-Mediated Shortening of Action Potentials in LQTS2 Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.

Stem cells and development

Duncan G,Firth K,George V,Hoang MD,Staniforth A,Smith G,Denning C

12-8813 was used in Flow cytometry/Cell sorting to investigate the importance of drug-mediated shortening of action potentials in cardiomyocytes from patients with LQTS2.

Fri Dec 01 00:00:00 UTC 2017

NANOG alone induces germ cells in primed epiblast in vitro by activation of enhancers.

Nature

Murakami K,Günesdogan U,Zylicz JJ,Tang WWC,Sengupta R,Kobayashi T,Kim S,Butler R,Dietmann S,Surani MA

Published figure using SSEA1 monoclonal antibody (Product # 12-8813-42) in Flow Cytometry

Thu Jan 21 00:00:00 UTC 2016

Parent stem cells can serve as niches for their daughter cells.

Nature

Pardo-Saganta A,Tata PR,Law BM,Saez B,Chow RD,Prabhu M,Gridley T,Rajagopal J

Published figure using SSEA1 monoclonal antibody (Product # 12-8813-42) in Flow Cytometry

Thu Jul 30 00:00:00 UTC 2015

Generation of embryonic stem cells from mouse adipose-tissue derived cells via somatic cell nuclear transfer.

Cell cycle (Georgetown, Tex.)

Qin Y,Qin J,Zhou C,Li J,Gao WQ

12-8813 was used in Flow cytometry/Cell sorting to demonstrates that ESCs can be generated from the adipose tissue by somatic cell nuclear transfer (SCNT) and suggests that ADCs can be a new donor-cell type for potential therapeutic cloning.

Tue Jan 26 00:00:00 UTC 2016

Coexpression analysis of CD133 and CD44 identifies proneural and mesenchymal subtypes of glioblastoma multiforme.

Oncotarget

Brown DV,Daniel PM,D'Abaco GM,Gogos A,Ng W,Morokoff AP,Mantamadiotis T

12-8813 was used in Flow cytometry/Cell sorting to identify two new potential protein markers for molecular glioma subtype.

Fri Mar 20 00:00:00 UTC 2015

Generation, expansion, and differentiation of cardiovascular progenitor cells from human pluripotent stem cells.

Methods in molecular biology (Clifton, N.J.)

Cao N,Liang H,Yang HT

12-8813-42 was used in Flow cytometry/Cell sorting to describe the protocols for efficient derivation, expansion, and differentiation of CVPCs from hPSCs in a chemically defined medium under feeder- and serum-free culture conditions.

Tue Feb 09 00:00:00 UTC 2016

Distinct and combinatorial functions of Jmjd2b/Kdm4b and Jmjd2c/Kdm4c in mouse embryonic stem cell identity.

Molecular cell

Das PP,Shao Z,Beyaz S,Apostolou E,Pinello L,De Los Angeles A,O'Brien K,Atsma JM,Fujiwara Y,Nguyen M,Ljuboja D,Guo G,Woo A,Yuan GC,Onder T,Daley G,Hochedlinger K,Kim J,Orkin SH

Published figure using SSEA1 monoclonal antibody (Product # 12-8813-42) in Flow Cytometry

Thu Jan 09 00:00:00 UTC 2014

Dax1 and Nanog act in parallel to stabilize mouse embryonic stem cells and induced pluripotency.

Nature communications

Zhang J,Liu G,Ruan Y,Wang J,Zhao K,Wan Y,Liu B,Zheng H,Peng T,Wu W,He P,Hu FQ,Jian R

Published figure using SSEA1 monoclonal antibody (Product # 12-8813-42) in Flow Cytometry

Mon Oct 06 00:00:00 UTC 2014

ΔNp63 regulates select routes of reprogramming via multiple mechanisms.

Cell death and differentiation

Alexandrova EM,Petrenko O,Nemajerova A,Romano RA,Sinha S,Moll UM

12-8813 was used in Flow cytometry/Cell sorting to report that in stark contrast to p53, p73 has no role in the molecular mechanisms of reprogramming the conversion of somatic cells into induced pluripotent stem cells.

Sun Dec 01 00:00:00 UTC 2013

Cell reprogramming requires silencing of a core subset of polycomb targets.

PLoS genetics

Fragola G,Germain PL,Laise P,Cuomo A,Blasimme A,Gross F,Signaroldi E,Bucci G,Sommer C,Pruneri G,Mazzarol G,Bonaldi T,Mostoslavsky G,Casola S,Testa G

Published figure using SSEA1 monoclonal antibody (Product # 12-8813-42) in Flow Cytometry

Thu Jun 13 00:00:00 UTC 2013

Embryonic stem cells require Wnt proteins to prevent differentiation to epiblast stem cells.

Nature cell biology

ten Berge D,Kurek D,Blauwkamp T,Koole W,Maas A,Eroglu E,Siu RK,Nusse R

Published figure using SSEA1 monoclonal antibody (Product # 12-8813-42) in Flow Cytometry

Sun Aug 14 00:00:00 UTC 2011

Three-dimensional cartography of hematopoietic clusters in the vasculature of whole mouse embryos.

Development (Cambridge, England)

Yokomizo T,Dzierzak E

12-8813 was used in Flow cytometry/Cell sorting to devise a method to make transparent whole mouse embryos for the observation of hematopoietic clusters.

Mon Nov 01 00:00:00 UTC 2010

Bioinformatics

Protein Aliases: 3-fucosyl-N-acetyl-lactosamine epitope; 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase; alpha (1,3) fucosyltransferase, myeloid specific; alpha (1,3) fucosyltransferase, myeloid-specific; alpha 1,3-fucosyltransferase; Alpha-(1,3)-fucosyltransferase 4; ELAM ligand fucosyltransferase; ELAM-1 ligand fucosyltransferase; Fuc-TIV; Fucosyltransferase 4; fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific); Fucosyltransferase IV; fucT-IV; FUTIV; Galactoside 3-L-fucosyltransferase; Lewis X; SSEA-1; stage-specific embryonic antigen 1

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Gene Aliases: AI451562; CD15; ELFT; FAL; FCT3A; FUC-TIV; FucT-IV; FUT4; FUTIV; LeX; SSEA-1; Ssea1

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UniProt ID: (Human) P22083, (Mouse) Q11127

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Entrez Gene ID: (Human) 2526, (Mouse) 14345

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Function(s)

Process(es)

carbohydrate metabolic process protein glycosylation protein N-linked glycosylation fucosylation L-fucose catabolic process

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SSEA1 Monoclonal Antibody (eBioMC-480 (MC-480)), PE, eBioscience™

SSEA1 Antibody (12-8813-42) in Flow

Product Details

12-8813-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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