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Invitrogen
STAM Polyclonal Antibody
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FIGURE: 1 / 5
STAM Antibody (PA5-144659) in ICC/IF
Immunocytochemistry analysis of STAM in SH-SY5Y cells. Cells were fixed in 4% paraformaldehyde for 30 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at 37&(8)451;. Cells were incubated with STAM polyclonal antibody (Product # PA5-144659) with a dilution of 1:200 in 2% BSA overnight at 4 &(8)451;. Followed by secondary antibody Goat Anti-Rabbit IgG H&L (iFluor™ 488) at a dilution of 1:1,000. Nuclear DNA was labelled in blue with DAPI, beta tubulin (red) wa... View More
Product Details
PA5-144659
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide within Human aa 30-55/540.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_3091363
Product Specific Information
Positive control: Siha, SHSY5Y, human kidney tissue, human colon tissue, Rat testis tissue.
Predicted band size: 59 kDa
Subcellular Location: Cytoplasm; Early endosome membrane.
Target Information
STAM (Signal transducing adaptor molecule) is an adaptor molecule involved in downstream signaling pathways mediated by growth factors and cytokines. STAM also plays an important role in endocytosis, the JAK-STAT signaling pathway, and membrane trafficking. In humans, the gene encoding STAM is located on chromosome 10.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp686J2352; HSE1 homolog; Signal transducing adapter molecule 1; signal transducing adaptor molecule (SH3 domain and ITAM motif) 1; STAM-1; STAT91
Gene Aliases: RGD1564499; STAM; STAM-1; STAM1
UniProt ID: (Human) Q92783
Entrez Gene ID: (Human) 8027, (Rat) 498798
intracellular protein transport
positive regulation of exosomal secretion
regulation of extracellular exosome assembly
autophagy
signal transduction
positive regulation of signal transduction
endosomal transport
multivesicular body assembly
negative regulation of epidermal growth factor receptor signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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