Invitrogen

STARD3 Polyclonal Antibody

FIGURE: 1 / 5

STARD3 Antibody (PA1-562) in WB

Western blot of MLN64 on rat adrenal gland extract using Product # PA1-562.

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Product Details

PA1-562

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 5 publications 5 publications

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Rat

Published species

Bovine, Hamster, Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant MLN64 protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2197799

Product Specific Information

PA1-562 detects MLN64 from rat tissues.

PA1-562 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~53 kDa protein representing MLN64 from rat adrenal gland tissue extracts. This antibody also detects an ~25 kDa protein from rat adrenal gland which may correspond to degradation product.

PA1-562 antigen is recombinant MLN64 protein.

Target Information

The steroidogenic acute regulatory (StAR) protein facilitates the movement of cholesterol from the outer to inner mitochondrial membrane in adrenal and gonadal cells, fostering steroid biosynthesis. MLN64 is a 445-amino acid protein of unknown function. When 218 amino-terminal residues of MLN64 are deleted, the resulting N-218 MLN64 has 37% amino acid identity with StAR and 50% of StAR's steroidogenic activity in transfected cells. Bacterially expressed N-218 MLN64 exerts StAR-like activity to promote the transfer of cholesterol from the outer to inner mitochondrial membrane in vitro. The presence of a protease-resistant domain and a protease-sensitive carboxy-terminal domain in N-218 MLN64 is similar to the organization of StAR. However, as MLN64 never enters the mitochondria, the protease-resistant domain of MLN64 cannot be a mitochondrial pause-transfer sequence, as has been proposed for StAR.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Proteomic Analysis of Niemann-Pick Type C Hepatocytes Reveals Potential Therapeutic Targets for Liver Damage.

Cells

Balboa E,Marín T,Oyarzún JE,Contreras PS,Hardt R,van den Bosch T,Alvarez AR,Rebolledo-Jaramillo B,Klein AD,Winter D,Zanlungo S

Published figure using STARD3 polyclonal antibody (Product # PA1-562) in Western Blot

Sat Aug 21 00:00:00 UTC 2021

Niemann-Pick Type C2 protein contributes to the transport of endosomal cholesterol to mitochondria without interacting with NPC1.

Journal of lipid research

Kennedy BE,Charman M,Karten B

PA1-562 was used in western blot to study the NPC1-independent transport of endosomal cholesterol to mitochondria by Niemann-Pick type C2 protein

Sat Dec 01 00:00:00 UTC 2012

Effects of interleukin-8 on estradiol and progesterone production by bovine granulosa cells from large follicles and progesterone production by luteinizing granulosa cells in culture.

Cytokine

Shimizu T,Kaji A,Murayama C,Magata F,Shirasuna K,Wakamiya K,Okuda K,Miyamoto A

PA1-562 was used in western blot to study the role of interleukin-8 in steroid synthesis in granulosa cells

Sun Jan 01 00:00:00 UTC 2012

BMP-4 suppresses progesterone production by inhibiting histone H3 acetylation of StAR in bovine granulosa cells in vitro.

Molecular and cellular biochemistry

Yamashita H,Murayama C,Takasugi R,Miyamoto A,Shimizu T

PA1-562 was used in immunocytochemistry to study the mechanism by which BMP-4 inhibits the production of progesterone and the expression of steroidogenic genes.

Tue Feb 01 00:00:00 UTC 2011

MLN64 mediates egress of cholesterol from endosomes to mitochondria in the absence of functional Niemann-Pick Type C1 protein.

Journal of lipid research

Charman M,Kennedy BE,Osborne N,Karten B

PA1-562 was used in western blot to investigate the role of endosomal metastatic lymph node protein 64 MLN64 in cholesterol mitochondrial transport

Sat May 01 00:00:00 UTC 2010

Trafficking from CD63-positive late endocytic multivesicular bodies is essential for intracellular development of Chlamydia trachomatis.

Journal of cell science

Beatty WL

PA1-562 was used in immunocytochemistry to study the intracellular mechanism for chlamydiae infection.

Sun Jan 15 00:00:00 UTC 2006

N-218 MLN64, a protein with StAR-like steroidogenic activity, is folded and cleaved similarly to StAR.

Biochemistry

Bose HS,Whittal RM,Huang MC,Baldwin MA,Miller WL

PA1-562 was used in western blot to investigate the functional properties of N-218 MLN64 in comparison with StAR

Tue Sep 26 00:00:00 UTC 2000

Bioinformatics

Protein Aliases: stAR-related lipid transfer protein 3; START domain containing 3

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Entrez Gene ID: (Rat) 363675