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          • Primary Antibodies ›
          • STAT1 Antibodies

          Proteintech

          STAT1 Polyclonal Antibody

          107 References
          View all (90) STAT1 antibodies

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          Datasheet
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          Datasheet
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          Cite STAT1 Polyclonal Antibody

          • Antibody Testing Data (16)
          STAT1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          STAT1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 16

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          STAT1 Antibody (10144-2-AP) in ICC/IF

          Immunofluorescent analysis of ( 10% Formaldehyde ) fixed PC-3 cells using 10144-2-AP (STAT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          STAT1 Antibody in Immunocytochemistry (ICC/IF)
          STAT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          STAT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          STAT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          STAT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          STAT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          STAT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          STAT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          STAT1 Antibody in Western Blot (WB)
          STAT1 Antibody in Western Blot (WB)
          STAT1 Antibody in Western Blot (WB)
          STAT1 Antibody in Western Blot (WB)
          STAT1 Antibody in Western Blot (WB)
          STAT1 Antibody in Western Blot (WB)
          STAT1 Antibody in Flow Cytometry (Flow)
          STAT1 Antibody in Immunoprecipitation (IP)

          Product Details

          10144-2-AP

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:1,000
          View 86 publications 86 publications

          Immunohistochemistry (IHC)

          -
          View 3 publications 3 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:20-1:200
          -

          Immunocytochemistry (ICC/IF)

          1:50-1:500
          View 9 publications 9 publications

          Flow Cytometry (Flow)

          0.2 µg/1x10^6 cells
          -

          Immunoprecipitation (IP)

          0.5-4.0 µg
          View 7 publications 7 publications

          ChIP assay (ChIP)

          -
          View 1 publication 1 publication

          Not applicable (N/A)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Rat

          Published species

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          STAT1 Fusion Protein Ag0199 (2-230 aa encoded by BC002704)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.13 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice (domestic); Dry ice (international)

          Product Specific Information

          This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human STAT1.

          Immunogen sequence: SQWYELQQL DSKFLEQVHQ LYDDSFPMEI RQYLAQWLEK QDWEHAANDV SFATIRFHDL LSQLDDQYSR FSLENNFLLQ HNIRKSKRNL QDNFQEDPIQ MSMIIYSCLK EERKILENAQ RFNQAQSGNI QSTVMLDKQK ELDSKVRNVK DKVMCIEHEI KSLEDLQDEY DFKCKTLQNR EHETNGVAKS DQKQEQLLLK KMYLMLDNKR KEVVHKIIEL LNVTELTQNA (2-230 aa encoded by BC002704)

          Target Information

          STAT1 (signal transducers and activators of transcription 1) is a member of the STAT family of transcription factors. STAT1 can be activated by interferon-alpha, interferon-gamma, EGF, PDGF and IL6. STAT1 is known to regulate several genes which are involved in cell growth, apoptosis, immune responses, and lipid metabolism. Further, STAT1 plays an important role in mediating cell viability in response to different cell stimuli and pathogen exposure. The STAT1 gene is located on chromosome 2. STAT1 is activated to regulate gene expression in response to extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STAT1 enters the nucleus to regulate transcription of many different genes. Among the seven STATs types, STAT1, STAT3, STAT5a, and STAT5b have a wide activation profile. STAT1 is activated by many different ligands including IFN family (IFN-Alpha, IFN-Beta, IFN-gamma and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-1). Two alternatively spliced transcript variants encoding distinct isoforms of STAT1 have been described.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: DKFZp686B04100; OTTHUMP00000165046; OTTHUMP00000205845; signal transducer and activator of transcription 1, 91kD; signal transducer and activator of transcription 1, 91kDa; Signal transducer and activator of transcription 1-alpha/beta; signal transducer and activator of transcription-1; STAT; STAT 1; STAT-1; stat1 alpha; Transcription factor ISGF-3 components p91/p84

          View more View less

          Gene Aliases: CANDF7; DD6G4-4; IMD31A; IMD31B; IMD31C; ISGF-3; STAT1; STAT91

          View more View less

          UniProt ID: (Human) P42224

          View more View less

          Entrez Gene ID: (Human) 6772, (Rat) 25124

          View more View less

          Function(s)
          RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity, RNA polymerase II core promoter sequence-specific double-stranded DNA binding transcription factor activity, sequence-specific DNA binding signal transducer activity tumor necrosis factor receptor binding protein binding enzyme binding nuclear hormone receptor binding identical protein binding protein homodimerization activity cadherin binding involved in cell-cell adhesion DNA binding CCR5 chemokine receptor binding sequence-specific DNA binding protein phosphatase 2A binding
          Process(es)
          negative regulation of endothelial cell proliferation transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter activation of cysteine-type endopeptidase activity involved in apoptotic process JAK-STAT cascade response to nutrient blood circulation positive regulation of cell proliferation response to mechanical stimulus response to bacterium response to organic cyclic compound negative regulation of angiogenesis cytokine-mediated signaling pathway lipopolysaccharide-mediated signaling pathway response to lipopolysaccharide cellular response to insulin stimulus tumor necrosis factor-mediated signaling pathway response to cytokine negative regulation of macrophage fusion cellular response to interferon-beta response to drug response to hydrogen peroxide negative regulation of I-kappaB kinase/NF-kappaB signaling response to exogenous dsRNA response to peptide hormone endothelial cell migration positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation by virus of viral protein levels in host cell positive regulation of smooth muscle cell proliferation response to cAMP interferon-gamma-mediated signaling pathway type I interferon signaling pathway renal tubule development cellular response to lipopolysaccharide negative regulation of transcription from RNA polymerase II promoter positive regulation of mesenchymal cell proliferation negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis apoptotic process macrophage derived foam cell differentiation response to interferon-beta regulation of apoptotic process defense response to virus regulation of interferon-gamma-mediated signaling pathway regulation of type I interferon-mediated signaling pathway cellular response to organic cyclic compound metanephric mesenchymal cell proliferation involved in metanephros development metanephric mesenchymal cell differentiation negative regulation of metanephric nephron tubule epithelial cell differentiation cell-cell adhesion
          It has to be done as per old AB suggested Products section.
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