Invitrogen

STAT1 Monoclonal Antibody (15H3)

STAT1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 7

STAT1 Antibody (MA1-037) in ICC/IF

Immunofluorescent analysis of STAT1 in MCF-7 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a STAT1 monoclonal antibody (Product # MA1-037) at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). STAT1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

STAT1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

STAT1 Antibody — The inhibition of the STAT1/STAT3 signaling reverses the kynurenine-dependent immunosuppression in multidrug resistant cells. A549/dx cells were grown for 48 h in fresh medium (CTRL), treated with a non-targeting scrambled siRNA (scr) or with a specific siRNAs pool targeting STAT1 or STAT3, respectively (si STAT1, si STAT3). Untreated chemosensitive A549 cells were used as control. A. The expression of STAT1, STAT3, IDO1, IDO2 and TDO was measured in whole cell lysates by Western blotting, 48 h after the transfection. The β-tubulin expression was used as control of equal protein loading. The figure is representative of 3 experiments with similar results. B. The kynurenine levels in the cell culture supernatants were measured spectrophotometrically. Data are presented as means SD (n = 4). * p < 0.01: versus A549 CTRL; ° p < 0.005, °° p < 0.001: versus A549/dx CTRL. C. The proliferation of activated T-lymphocytes collected from PBMC after a 72 h co-incubation with A549 and A549/dx cells was measured with the [3H]thymidine assay. In the presence of anti-CD3 and anti-CD28 stimulatory antibodies without tumor cells (positive control), the [3H]thymidine incorporation was 28,926 1,426 cpm; in the presence of RPMI medium alone (negative control), the [3H]thymidine incorporation was 4,312 529 cpm. Data are presented as means SD (n = 6). * p < 0.05: versus A549 CTRL; ° p < 0.01, °° p < 0.005: versus A549/dx CTRL. D. The percentage of CD3+ T-lymphocytes colle {KD}

Product Details

MA1-037

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1:20

Immunoprecipitation (IP)

2 µg

Product Specifications

Species Reactivity

Human, Non-human primate

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

15H3

Immunogen

Protein expressed in 293T cell transfected with human STAT1 expression vector.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA, 30% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11154072

Product Specific Information

Note: This antibody requires overnight incubation in a Western blot for optimal performance. Do not use this antibody in a Fast Western blotting procedure.

Target Information

STAT1 (signal transducers and activators of transcription 1) is a member of the STAT family of transcription factors. STAT1 can be activated by interferon-alpha, interferon-gamma, EGF, PDGF and IL6. STAT1 is known to regulate several genes which are involved in cell growth, apoptosis, immune responses, and lipid metabolism. Further, STAT1 plays an important role in mediating cell viability in response to different cell stimuli and pathogen exposure. The STAT1 gene is located on chromosome 2. STAT1 is activated to regulate gene expression in response to extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STAT1 enters the nucleus to regulate transcription of many different genes. Among the seven STATs types, STAT1, STAT3, STAT5a, and STAT5b have a wide activation profile. STAT1 is activated by many different ligands including IFN family (IFN-Alpha, IFN-Beta, IFN-gamma and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-1). Two alternatively spliced transcript variants encoding distinct isoforms of STAT1 have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A multi-shRNA vector enhances the silencing efficiency of exogenous and endogenous genes in human cells.

Oncology letters

Weng Y,Shi Y,Xia X,Zhou W,Wang H,Wang C

MA1037X was used in western blot to develop and test a multi-shRNA RNAi vector

Wed Mar 01 00:00:00 UTC 2017

Dipeptidyl Peptidase-4 and Adolescent Idiopathic Scoliosis: Expression in Osteoblasts.

Scientific reports

Normand E,Franco A,Moreau A,Marcil V

MA1-037X was used in Western Blotting to suggest a role for incretins in AIS development and severity.

Fri Jun 09 00:00:00 UTC 2017

Dipeptidyl Peptidase-4 and Adolescent Idiopathic Scoliosis: Expression in Osteoblasts.

Scientific reports

Normand E,Franco A,Moreau A,Marcil V

MA1-037X was used in Western Blotting to suggest a role for incretins in AIS development and severity.

Fri Jun 09 00:00:00 UTC 2017

An Autocrine Cytokine/JAK/STAT-Signaling Induces Kynurenine Synthesis in Multidrug Resistant Human Cancer Cells.

PloS one

Campia I,Buondonno I,Castella B,Rolando B,Kopecka J,Gazzano E,Ghigo D,Riganti C

MA1-037 was used in western blot to analyze multidrug resistant human cancer cells and autocrime cytokine/JAK/STAT-signaling induces kynurenin synthesis

Tue Feb 02 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: DKFZp686B04100; OTTHUMP00000165046; OTTHUMP00000205845; signal transducer and activator of transcription 1, 91kD; signal transducer and activator of transcription 1, 91kDa; Signal transducer and activator of transcription 1-alpha/beta; signal transducer and activator of transcription-1; STAT; STAT 1; STAT-1; stat1 alpha; Transcription factor ISGF-3 components p91/p84

View more View less

Gene Aliases: CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT1; STAT91

View more View less

UniProt ID: (Human) P42224

View more View less

Entrez Gene ID: (Human) 6772

View more View less

Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity, RNA polymerase II core promoter sequence-specific double-stranded DNA binding transcription factor activity, sequence-specific DNA binding signal transducer activity tumor necrosis factor receptor binding protein binding enzyme binding nuclear hormone receptor binding identical protein binding protein homodimerization activity cadherin binding involved in cell-cell adhesion

Process(es)

negative regulation of transcription from RNA polymerase II promoter negative regulation of endothelial cell proliferation positive regulation of mesenchymal cell proliferation negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis transcription, DNA-templated apoptotic process JAK-STAT cascade blood circulation macrophage derived foam cell differentiation negative regulation of angiogenesis tumor necrosis factor-mediated signaling pathway response to cytokine response to interferon-beta cellular response to interferon-beta regulation of apoptotic process negative regulation of I-kappaB kinase/NF-kappaB signaling response to peptide hormone endothelial cell migration positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation by virus of viral protein levels in host cell positive regulation of smooth muscle cell proliferation response to cAMP defense response to virus interferon-gamma-mediated signaling pathway regulation of interferon-gamma-mediated signaling pathway type I interferon signaling pathway regulation of type I interferon-mediated signaling pathway renal tubule development cellular response to organic cyclic compound metanephric mesenchymal cell proliferation involved in metanephros development metanephric mesenchymal cell differentiation negative regulation of metanephric nephron tubule epithelial cell differentiation cell-cell adhesion

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

STAT1 Monoclonal Antibody (15H3)

STAT1 Antibody (MA1-037) in ICC/IF

Product Details

MA1-037

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!