Invitrogen

STAT1 Monoclonal Antibody (STAT1-79)

STAT1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 22

STAT1 Antibody (AHO0832) in ICC/IF

Immunofluorescent analysis of STAT-1 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with STAT1 Antibody (STAT1-79) (Product # AHO0832) at 1:250 dilution in1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Rabbit Anti-Mouse IgG Secondary Antibody (Product # A-11059) at a dilution of 1:400 for 45 minutes at ... View More

STAT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

STAT1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

STAT1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with STAT1 siRNA and decrease in signal intensity was observed in western blot application (Fig a) using STAT1 monoclonal Antibody (Product # AHO0832). Densitometric analysis of this western blot is shown in histogram (Fig b). {KD}

Product Details

AHO0832

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 10 publications 10 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

Immunocytochemistry (ICC/IF)

1:250

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-1 µg/mL

View 1 publication 1 publication

ChIP assay (ChIP)

5 µL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Virus

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

STAT1-79

Immunogen

Recombinant human STAT1-alpha expressed in E. coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.2, with 1% BSA

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2536317

Target Information

STAT1 (signal transducers and activators of transcription 1) is a member of the STAT family of transcription factors. STAT1 can be activated by interferon-alpha, interferon-gamma, EGF, PDGF and IL6. STAT1 is known to regulate several genes which are involved in cell growth, apoptosis, immune responses, and lipid metabolism. Further, STAT1 plays an important role in mediating cell viability in response to different cell stimuli and pathogen exposure. The STAT1 gene is located on chromosome 2. STAT1 is activated to regulate gene expression in response to extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STAT1 enters the nucleus to regulate transcription of many different genes. Among the seven STATs types, STAT1, STAT3, STAT5a, and STAT5b have a wide activation profile. STAT1 is activated by many different ligands including IFN family (IFN-Alpha, IFN-Beta, IFN-gamma and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-1). Two alternatively spliced transcript variants encoding distinct isoforms of STAT1 have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Type I and III interferons shape the retinal cytokine network and barrier function in an in vitro model of ocular toxoplasmosis.

Frontiers in immunology

Geiller B,Greigert V,Hillenbrand CA,Gommenginger C,Beal L,Brunet J,Filisetti D,Villard O,Denis J,Pfaff AW

AHO0832 was used in Western Blotting to show that stimulation with type I and III interferons did not limit parasite proliferation in retinal cells we tested.

Mon May 22 00:00:00 UTC 2023

Inhibition of the IFN-α JAK/STAT Pathway by MERS-CoV and SARS-CoV-1 Proteins in Human Epithelial Cells.

Viruses

Zhang Y,Gargan S,Roche FM,Frieman M,Stevenson NJ

AHO0832 was used in Immunocytochemistry-immunofluorescence to show that in lung alveolar A549 epithelial cells expression of MERS-CoV-nsp2 and SARS-CoV-1-nsp14, but not MERS-CoV-nsp5, increased basal levels of total and phosphorylated STAT1 STAT2 protein, but reduced IFN-α-mediated phosphorylation of STAT1-3 and induction of MxA.

Wed Mar 23 00:00:00 UTC 2022

Inhibition of the IFN-α JAK/STAT Pathway by MERS-CoV and SARS-CoV-1 Proteins in Human Epithelial Cells.

Viruses

Zhang Y,Gargan S,Roche FM,Frieman M,Stevenson NJ

Wed Mar 23 00:00:00 UTC 2022

Histone methylation antagonism drives tumor immune evasion in squamous cell carcinomas.

Molecular cell

Li Y,Goldberg EM,Chen X,Xu X,McGuire JT,Leuzzi G,Karagiannis D,Tate T,Farhangdoost N,Horth C,Dai E,Li Z,Zhang Z,Izar B,Que J,Ciccia A,Majewski J,Yoon AJ,Ailles L,Mendelsohn CL,Lu C

AHO0832 was used in Western Blot to report that inactivation of the histone H3K36 methyltransferase NSD1, which is frequently found in squamous cell carcinomas (SCCs) and induces DNA hypomethylation, unexpectedly results in diminished tumor immune infiltration.

Thu Oct 20 00:00:00 UTC 2022

Amyloid-beta and tau pathologies act synergistically to induce novel disease stage-specific microglia subtypes.

Molecular neurodegeneration

Kim DW,Tu KJ,Wei A,Lau AJ,Gonzalez-Gil A,Cao T,Braunstein K,Ling JP,Troncoso JC,Wong PC,Blackshaw S,Schnaar RL,Li T

AHO0832 was used in ChIP Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to suggest that both Aß and tau pathologies are required for the disease stage-specific induction of early-stage AD-Associated Microglia (EADAM) and late-stage AD-Associated Microglia (LADAM), and reveal Siglecs as biomarkers of Alzheimer's disease progression.

Sat Dec 17 00:00:00 UTC 2022

MiR-342 controls Mycobacterium tuberculosis susceptibility by modulating inflammation and cell death.

EMBO reports

Fu B,Lin X,Tan S,Zhang R,Xue W,Zhang H,Zhang S,Zhao Q,Wang Y,Feldman K,Shi L,Zhang S,Nian W,Chaitanya Pavani K,Li Z,Wang X,Wu H

Published figure using STAT1 monoclonal antibody (Product # AHO0832) in Western Blot

Mon Sep 06 00:00:00 UTC 2021

SARS-CoV-2 suppresses IFNβ production mediated by NSP1, 5, 6, 15, ORF6 and ORF7b but does not suppress the effects of added interferon.

PLoS pathogens

Shemesh M,Aktepe TE,Deerain JM,McAuley JL,Audsley MD,David CT,Purcell DFJ,Urin V,Hartmann R,Moseley GW,Mackenzie JM,Schreiber G,Harari D

Published figure using STAT1 monoclonal antibody (Product # AHO0832) in Western Blot

Sun Aug 01 00:00:00 UTC 2021

Metabolites Profiling of Melanoma Interstitial Fluids Reveals Uridine Diphosphate as Potent Immune Modulator Capable of Limiting Tumor Growth.

Frontiers in cell and developmental biology

Vecchio E,Caiazza C,Mimmi S,Avagliano A,Iaccino E,Brusco T,Nisticò N,Maisano D,Aloisio A,Quinto I,Renna M,Divisato G,Romano S,Tufano M,D'Agostino M,Vigliar E,Iaccarino A,Mignogna C,Andreozzi F,Mannino GC,Spiga R,Stornaiuolo M,Arcucci A,Mallardo M,Fiume G

AHO0832 was used in Western Blot to deploy a metabolomic approach to analyze the composition of melanoma tumor interstitial fluid and compared it to the plasma of C57BL6 mice, engrafted or not with B16-melanoma cells.

Wed Apr 03 00:00:00 UTC 2024

Metabolites Profiling of Melanoma Interstitial Fluids Reveals Uridine Diphosphate as Potent Immune Modulator Capable of Limiting Tumor Growth.

Frontiers in cell and developmental biology

Wed Apr 03 00:00:00 UTC 2024

Infant High-Grade Gliomas Comprise Multiple Subgroups Characterized by Novel Targetable Gene Fusions and Favorable Outcomes.

Cancer discovery

Clarke M,Mackay A,Ismer B,Pickles JC,Tatevossian RG,Newman S,Bale TA,Stoler I,Izquierdo E,Temelso S,Carvalho DM,Molinari V,Burford A,Howell L,Virasami A,Fairchild AR,Avery A,Chalker J,Kristiansen M,Haupfear K,Dalton JD,Orisme W,Wen J,Hubank M,Kurian KM,Rowe C,Maybury M,Crosier S,Knipstein J,Schüller U,Kordes U,Kram DE,Snuderl M,Bridges L,Martin AJ,Doey LJ,Al-Sarraj S,Chandler C,Zebian B,Cairns C,Natrajan R,Boult JKR,Robinson SP,Sill M,Dunkel IJ,Gilheeney SW,Rosenblum MK,Hughes D,Proszek PZ,Macdonald TJ,Preusser M,Haberler C,Slavc I,Packer R,Ng HK,Caspi S,Popović M,Faganel Kotnik B,Wood MD,Baird L,Davare MA,Solomon DA,Olsen TK,Brandal P,Farrell M,Cryan JB,Capra M,Karremann M,Schittenhelm J,Schuhmann MU,Ebinger M,Dinjens WNM,Kerl K,Hettmer S,Pietsch T,Andreiuolo F,Driever PH,Korshunov A,Hiddingh L,Worst BC,Sturm D,Zuckermann M,Witt O,Bloom T,Mitchell C,Miele E,Colafati GS,Diomedi-Camassei F,Bailey S,Moore AS,Hassall TEG,Lowis SP,Tsoli M,Cowley MJ,Ziegler DS,Karajannis MA,Aquilina K,Hargrave DR,Carceller F,Marshall LV,von Deimling A,Kramm CM,Pfister SM,Sahm F,Baker SJ,Mastronuzzi A,Carai A,Vinci M,Capper D,Popov S,Ellison DW,Jacques TS,Jones DTW,Jones C

AHO0832 was used in Immunocytochemistry to collect the largest series of infant gliomas (exclusive of pilocytic astrocytomas) assembled to date and present a classification system based on integrated methylation profiling, fusion gene analysis, mutation detection, and histologic review, with preclinical and clinical evidence of effective targeting of the driving alterations in these unique entities.

Wed Jul 01 00:00:00 UTC 2020

STAT1 expression and HPV16 viral load predict cervical lesion progression.

Oncology letters

Wu S,Wu Y,Lu Y,Yue Y,Cui C,Yu M,Wang S,Liu M,Zhao Y,Sun Z

AHO0832 was used in Immunohistochemistry to determine the association between STAT1 and human papillomavirus (HPV)16 in cervical lesions.

Thu Oct 01 00:00:00 UTC 2020

PTPN14 aggravates inflammation through promoting proteasomal degradation of SOCS7 in acute liver failure.

Cell death & disease

Fu B,Yin S,Lin X,Shi L,Wang Y,Zhang S,Zhao Q,Li Z,Yang Y,Wu H

Published figure using STAT1 monoclonal antibody (Product # AHO0832) in Western Blot

Fri Sep 25 00:00:00 UTC 2020

MicroRNA-325-3p Facilitates Immune Escape of Mycobacterium tuberculosis through Targeting LNX1 via NEK6 Accumulation to Promote Anti-Apoptotic STAT3 Signaling.

mBio

Fu B,Xue W,Zhang H,Zhang R,Feldman K,Zhao Q,Zhang S,Shi L,Pavani KC,Nian W,Lin X,Wu H

AHO0832 was used in Western Blotting to contribution to the understanding of the immune escape of M. tuberculosis, and they provide a theoretical basis for the development of therapeutic approaches for drug-resistant TB.

Tue Jun 02 00:00:00 UTC 2020

MicroRNA-325-3p Facilitates Immune Escape of Mycobacterium tuberculosis through Targeting LNX1 via NEK6 Accumulation to Promote Anti-Apoptotic STAT3 Signaling.

mBio

Fu B,Xue W,Zhang H,Zhang R,Feldman K,Zhao Q,Zhang S,Shi L,Pavani KC,Nian W,Lin X,Wu H

Tue Jun 02 00:00:00 UTC 2020

LncRNA PLAC2 down-regulates RPL36 expression and blocks cell cycle progression in glioma through a mechanism involving STAT1.

Journal of cellular and molecular medicine

Hu YW,Kang CM,Zhao JJ,Nie Y,Zheng L,Li HX,Li X,Wang Q,Qiu YR

Published figure using STAT1 monoclonal antibody (Product # AHO0832) in Immunoprecipitation

Mon Jan 01 00:00:00 UTC 2018

LncRNA PLAC2 down-regulates RPL36 expression and blocks cell cycle progression in glioma through a mechanism involving STAT1.

Journal of cellular and molecular medicine

Hu YW,Kang CM,Zhao JJ,Nie Y,Zheng L,Li HX,Li X,Wang Q,Qiu YR

Published figure using STAT1 monoclonal antibody (Product # AHO0832) in Immunoprecipitation

Mon Jan 01 00:00:00 UTC 2018

Endothelial IL-33 Expression Is Augmented by Adenoviral Activation of the DNA Damage Machinery.

Journal of immunology (Baltimore, Md. : 1950)

Stav-Noraas TE,Edelmann RJ,Poulsen LC,Sundnes O,Phung D,Küchler AM,Müller F,Kamen AA,Haraldsen G,Kaarbø M,Hol J

AHO0832 was used in western blot to identify factors that contribute to adenoviral transduction stimulation of IL-33 in endothelial cells

Sat Apr 15 00:00:00 UTC 2017

PDlim2 selectively interacts with the PDZ binding motif of highly pathogenic avian H5N1 influenza A virus NS1.

PloS one

Yu J,Li X,Wang Y,Li B,Li H,Li Y,Zhou W,Zhang C,Wang Y,Rao Z,Bartlam M,Cao Y

Mon Nov 28 00:00:00 UTC 2011

PDlim2 selectively interacts with the PDZ binding motif of highly pathogenic avian H5N1 influenza A virus NS1.

PloS one

Yu J,Li X,Wang Y,Li B,Li H,Li Y,Zhou W,Zhang C,Wang Y,Rao Z,Bartlam M,Cao Y

Mon Nov 28 00:00:00 UTC 2011

Bioinformatics

Protein Aliases: DKFZp686B04100; OTTHUMP00000165046; OTTHUMP00000205845; signal transducer and activator of transcription 1; signal transducer and activator of transcription 1, 91kD; signal transducer and activator of transcription 1, 91kDa; Signal transducer and activator of transcription 1-alpha/beta; signal transducer and activator of transcription-1; STAT; STAT 1; STAT-1; stat1 alpha; Transcription factor ISGF-3 components p91/p84

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Gene Aliases: 2010005J02Rik; AA408197; CANDF7; DD6G4-4; IMD31A; IMD31B; IMD31C; ISGF-3; STAT1; STAT91

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UniProt ID: (Human) P42224

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Entrez Gene ID: (Human) 6772, (Mouse) 20846, (Rat) 25124

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity, RNA polymerase II core promoter sequence-specific double-stranded DNA binding transcription factor activity, sequence-specific DNA binding signal transducer activity tumor necrosis factor receptor binding protein binding enzyme binding nuclear hormone receptor binding identical protein binding protein homodimerization activity cadherin binding involved in cell-cell adhesion DNA binding CCR5 chemokine receptor binding sequence-specific DNA binding protein phosphatase 2A binding

Process(es)

negative regulation of endothelial cell proliferation transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter activation of cysteine-type endopeptidase activity involved in apoptotic process signal transduction JAK-STAT cascade response to nutrient blood circulation positive regulation of cell proliferation response to mechanical stimulus response to bacterium negative regulation of angiogenesis cytokine-mediated signaling pathway lipopolysaccharide-mediated signaling pathway response to lipopolysaccharide cellular response to insulin stimulus tumor necrosis factor-mediated signaling pathway response to cytokine negative regulation of macrophage fusion cellular response to interferon-beta response to drug negative regulation of I-kappaB kinase/NF-kappaB signaling response to exogenous dsRNA response to peptide hormone positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation by virus of viral protein levels in host cell positive regulation of smooth muscle cell proliferation response to cAMP interferon-gamma-mediated signaling pathway type I interferon signaling pathway renal tubule development cellular response to lipopolysaccharide regulation of transcription from RNA polymerase II promoter response to organic cyclic compound response to hydrogen peroxide endothelial cell migration negative regulation of transcription from RNA polymerase II promoter positive regulation of mesenchymal cell proliferation negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis apoptotic process macrophage derived foam cell differentiation response to interferon-beta regulation of apoptotic process defense response to virus regulation of interferon-gamma-mediated signaling pathway regulation of type I interferon-mediated signaling pathway cellular response to organic cyclic compound metanephric mesenchymal cell proliferation involved in metanephros development metanephric mesenchymal cell differentiation negative regulation of metanephric nephron tubule epithelial cell differentiation cell-cell adhesion

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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STAT1 Monoclonal Antibody (STAT1-79)

STAT1 Antibody (AHO0832) in ICC/IF

Product Details

AHO0832

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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