Invitrogen

STAT5 beta Monoclonal Antibody (ST5b-10G1)

STAT5 beta Antibody in Immunocytochemistry (ICC/IF)

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STAT5 beta Antibody (13-5300) in ICC/IF

Immunofluorescent analysis of STAT5 beta was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with STAT5 beta Mouse Monoclonal Antibody (Product # 13-5300) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room... View More

STAT5 beta Antibody in Immunohistochemistry (Paraffin) (IHC (P))

STAT5 beta Antibody in Western Blot (WB)

STAT5 beta Antibody in ChIP Assay (ChIP)

STAT5 beta Antibody in Western Blot, Immunoprecipitation (WB, IP)

STAT5 beta Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

13-5300

Applications

Tested Dilution

Publications

Western Blot (WB)

1:250

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunohistochemistry - Free Floating (IHC (Free))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:250

View 2 publications 2 publications

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

Assay-dependent

View 3 publications 3 publications

ChIP assay (ChIP)

5 µg/mL

View 3 publications 3 publications

Gel Shift (GS)

Assay-dependent

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Bovine, Human, Mouse, Virus

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

ST5b-10G1

Immunogen

A peptide corresponding to the unique carboxy-terminus of murine STAT5b coupled to a protein carrier.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533021

Target Information

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. This gene was found to fuse to retinoic acid receptor-alpha (RARA) gene in a small subset of acute promyelocytic leukemias (APLL). The dysregulation of the signaling pathways mediated by this protein may be the cause of the APLL.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

IFN-β rescues neurodegeneration by regulating mitochondrial fission via STAT5, PGAM5, and Drp1.

The EMBO journal

Tresse E,Riera-Ponsati L,Jaberi E,Sew WQG,Ruscher K,Issazadeh-Navikas S

Published figure using STAT5 beta monoclonal antibody (Product # 13-5300) in Immunocytochemistry

Tue Jun 01 00:00:00 UTC 2021

Inhibition of the JAK/STAT Signaling Pathway in Regulatory T Cells Reveals a Very Dynamic Regulation of Foxp3 Expression.

PloS one

Goldstein JD,Burlion A,Zaragoza B,Sendeyo K,Polansky JK,Huehn J,Piaggio E,Salomon BL,Marodon G

135300 was used in ChIP assay to explore the role of the JAK/STAT pathway in controlling Foxp3 in regulatory T cells

Thu Aug 25 00:00:00 UTC 2016

Identification of STAT5A and STAT5B target genes in human T cells.

PloS one

Kanai T,Seki S,Jenks JA,Kohli A,Kawli T,Martin DP,Snyder M,Bacchetta R,Nadeau KC

13-5300 was used in ChIP-seq, immunocytochemistry, and western blot to investigate genes regulated by STAT5A and/or STAT5B in human CD4(+) T cells.

Mon Dec 22 00:00:00 UTC 2014

DNA methylation and transcription in a distal region upstream from the bovine AlphaS1 casein gene after once or twice daily milking.

PloS one

Nguyen M,Boutinaud M,Pétridou B,Gabory A,Pannetier M,Chat S,Bouet S,Jouneau L,Jaffrezic F,Laloë D,Klopp C,Brun N,Kress C,Jammes H,Charlier M,Devinoy E

13-5300 was used in EMSA to investigate the role of CpG methylation in milk production

Thu Jun 25 00:00:00 UTC 2015

Prolactin signalling in the mouse hypothalamus is primarily mediated by signal transducer and activator of transcription factor 5b but not 5a.

Journal of neuroendocrinology

Yip SH,Eguchi R,Grattan DR,Bunn SJ

13-5300 was used in immunohistochemistry - free floating to report that STAT5b and not STAT5a is the primary mediator prolactin signaling in the hypothalamus.

Sat Dec 01 00:00:00 UTC 2012

Identification of human STAT5-dependent gene regulatory elements based on interspecies homology.

The Journal of biological chemistry

Nelson EA,Walker SR,Li W,Liu XS,Frank DA

13-5300 was used in immunoprecipitation and western blot to identify NCAM1 as a novel STAT5 target

Fri Sep 08 00:00:00 UTC 2006

Identification of human STAT5-dependent gene regulatory elements based on interspecies homology.

The Journal of biological chemistry

Nelson EA,Walker SR,Li W,Liu XS,Frank DA

13-5300 was used in immunoprecipitation and western blot to identify NCAM1 as a novel STAT5 target

Fri Sep 08 00:00:00 UTC 2006

Identification of human STAT5-dependent gene regulatory elements based on interspecies homology.

The Journal of biological chemistry

Nelson EA,Walker SR,Li W,Liu XS,Frank DA

13-5300 was used in immunoprecipitation and western blot to identify NCAM1 as a novel STAT5 target

Fri Sep 08 00:00:00 UTC 2006

Identification of human STAT5-dependent gene regulatory elements based on interspecies homology.

The Journal of biological chemistry

Nelson EA,Walker SR,Li W,Liu XS,Frank DA

13-5300 was used in immunoprecipitation and western blot to identify NCAM1 as a novel STAT5 target

Fri Sep 08 00:00:00 UTC 2006

Identification of human STAT5-dependent gene regulatory elements based on interspecies homology.

The Journal of biological chemistry

Nelson EA,Walker SR,Li W,Liu XS,Frank DA

13-5300 was used in immunoprecipitation and western blot to identify NCAM1 as a novel STAT5 target

Fri Sep 08 00:00:00 UTC 2006

Activated signal transducer and activator of transcription (STAT) 3: localization in focal adhesions and function in ovarian cancer cell motility.

Cancer research

Silver DL,Naora H,Liu J,Cheng W,Montell DJ

Sat May 15 00:00:00 UTC 2004

Activated signal transducer and activator of transcription (STAT) 3: localization in focal adhesions and function in ovarian cancer cell motility.

Cancer research

Silver DL,Naora H,Liu J,Cheng W,Montell DJ

Sat May 15 00:00:00 UTC 2004

Activated signal transducer and activator of transcription (STAT) 3: localization in focal adhesions and function in ovarian cancer cell motility.

Cancer research

Silver DL,Naora H,Liu J,Cheng W,Montell DJ

Sat May 15 00:00:00 UTC 2004

Stat5a is tyrosine phosphorylated and nuclear localized in a high proportion of human breast cancers.

International journal of cancer

Cotarla I,Ren S,Zhang Y,Gehan E,Singh B,Furth PA

13-5300 was used in immunoprecipitation and western blot to examine the expression, tyrosine phosphorylation status and nuclear localization of Stat5a in human breast cancers.

Fri Feb 20 00:00:00 UTC 2004

Isolation of unique STAT5 targets by chromatin immunoprecipitation-based gene identification.

The Journal of biological chemistry

Nelson EA,Walker SR,Alvarez JV,Frank DA

13-5300 was used in western blot to investigate STAT5-regulated genes

Fri Dec 24 00:00:00 UTC 2004

Isolation of unique STAT5 targets by chromatin immunoprecipitation-based gene identification.

The Journal of biological chemistry

Nelson EA,Walker SR,Alvarez JV,Frank DA

13-5300 was used in western blot to investigate STAT5-regulated genes

Fri Dec 24 00:00:00 UTC 2004

Isolation of unique STAT5 targets by chromatin immunoprecipitation-based gene identification.

The Journal of biological chemistry

Nelson EA,Walker SR,Alvarez JV,Frank DA

13-5300 was used in western blot to investigate STAT5-regulated genes

Fri Dec 24 00:00:00 UTC 2004

Suppressor of cytokine signaling-1 is a critical regulator of interleukin-7-dependent CD8+ T cell differentiation.

Immunity

Chong MM,Cornish AL,Darwiche R,Stanley EG,Purton JF,Godfrey DI,Hilton DJ,Starr R,Alexander WS,Kay TW

Tue Apr 01 00:00:00 UTC 2003

Hormone-induced modifications of the chromatin structure surrounding upstream regulatory regions conserved between the mouse and rabbit whey acidic protein genes.

The Biochemical journal

Millot B,Montoliu L,Fontaine ML,Mata T,Devinoy E

13-5300 was used in Electrophoretic mobility shift assay to study the upstream regulatory regions of the mouse and rabbit whey acidic protein (WAP) genes to elucidate fully the mechanisms controlling WAP gene expression.

Thu May 15 00:00:00 UTC 2003

Bioinformatics

Protein Aliases: mammary gland factor STAT5B; Signal transducer and activator of transcription 5a; Signal transducer and activator of transcription 5B; STA5B; STAT; transcription factor STAT5B

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Gene Aliases: STAT5; STAT5B

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UniProt ID: (Human) P51692, (Rat) P52632, (Mouse) P42232

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Entrez Gene ID: (Human) 6777, (Rat) 25126, (Mouse) 20851

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Function(s)

RNA polymerase II core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding chromatin binding transcription factor activity, sequence-specific DNA binding protein tyrosine kinase activity signal transducer activity protein binding protein phosphatase binding glucocorticoid receptor binding protein dimerization activity DNA binding double-stranded DNA binding sequence-specific DNA binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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STAT5 beta Monoclonal Antibody (ST5b-10G1)

STAT5 beta Antibody (13-5300) in ICC/IF

Product Details

13-5300

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry - Free Floating (IHC (Free))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Gel Shift (GS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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