Invitrogen

STIM1 Monoclonal Antibody (CDN3H4)

7 Published Figures

8 References

View all (32) STIM1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)
Published Figures (7)

STIM1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 9

STIM1 Antibody (MA1-19451) in ICC/IF

Immunocytochemistry staining of HeLa human cervix carcinoma cell line using anti-STIM1 (CDN3H4; methanol-aceton fixation; detection by Goat anti-mouse IgG1 Alexa Fluor® 598; red) Monoclonal antibody (Product # MA1-19451). Nuclei were stained with DAPI (blue).

STIM1 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

Product Image Gallery

Product Details

MA1-19451

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL

View 1 publication 1 publication

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent

Immunoprecipitation (IP)

Assay-dependent

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

CDN3H4

Immunogen

Synthetic peptide corresponding to the C-terminal cytoplasmic part of STIM1

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

4°C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2197884

Product Specific Information

This antibody reacts with a cytoplasmic epitope of human and rodent STIM1, a 84 kDa essential and conserved regulator of store-operated Ca2+ channel function.

Western Blot: Both reducing and non-reducing conditions; Immunocytochemistry: Methanol-acetone fixation.

Target Information

STIM1 (stromal interacting molecule; also known as GOK) acts as a sensor of calcium depletion within the endoplasmic reticulum and transduces the signal to Orai1, the presumptive CRAC channel at the plasma membrane. Following decrease of luminal calcium concentration, STIM1 oligomerizes and induces Orai1 to enable entry of extracellular calcium into the cytoplasm. However, the precise mechanism of STIM1-Orai1 interaction has not been elucidated yet. Many questions also remain to be solved around STIM1 functional distribution. It turns out that STIM1 associates with growing ends of microtubules and is involved in endoplasmic reticulum tubule extension.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Proteomic Analyses of the G Protein-Coupled Estrogen Receptor GPER1 Reveal Constitutive Links to Endoplasmic Reticulum, Glycosylation, Trafficking, and Calcium Signaling.

Cells

Ahmadian Elmi M,Motamed N,Picard D

MA1-19451 was used in Western Blot to show that the Ca2+ sensor STIM1 interacts with GPER1 and that upon STIM1 overexpression and depletion of Ca2+ stores, GPER1 becomes more nuclear.

Fri Nov 03 00:00:00 UTC 2023

Proteomic Analyses of the G Protein-Coupled Estrogen Receptor GPER1 Reveal Constitutive Links to Endoplasmic Reticulum, Glycosylation, Trafficking, and Calcium Signaling.

Cells

Ahmadian Elmi M,Motamed N,Picard D

MA1-19451 was used in Western Blot to show that the Ca2+ sensor STIM1 interacts with GPER1 and that upon STIM1 overexpression and depletion of Ca2+ stores, GPER1 becomes more nuclear.

Fri Nov 03 00:00:00 UTC 2023

Immune Synapse Residency of Orai1 Alters Ca²⁺ Response of T Cells.

International journal of molecular sciences

Voros O,Panyi G,Hajdu P

Published figure using STIM1 monoclonal antibody (Product # MA1-19451) in Western Blot

Tue Oct 26 00:00:00 UTC 2021

Intracellular calcium leak lowers glucose storage in human muscle, promoting hyperglycemia and diabetes.

eLife

Tammineni ER,Kraeva N,Figueroa L,Manno C,Ibarra CA,Klip A,Riazi S,Rios E

MA1-19451 was used in Immunohistochemistry to reveal that Malignant Hyperthermia Susceptibility entails a wide-ranging, pathogenic change in glucose metabolism.

Mon May 04 00:00:00 UTC 2020

Intracellular calcium leak lowers glucose storage in human muscle, promoting hyperglycemia and diabetes.

eLife

Tammineni ER,Kraeva N,Figueroa L,Manno C,Ibarra CA,Klip A,Riazi S,Rios E

MA1-19451 was used in Immunohistochemistry to reveal that Malignant Hyperthermia Susceptibility entails a wide-ranging, pathogenic change in glucose metabolism.

Mon May 04 00:00:00 UTC 2020

STIM1- and Orai1-mediated Ca(2+) oscillation orchestrates invadopodium formation and melanoma invasion.

The Journal of cell biology

Sun J,Lu F,He H,Shen J,Messina J,Mathew R,Wang D,Sarnaik AA,Chang WC,Kim M,Cheng H,Yang S

MA1-19451 was used in immunohistochemistry - paraffin section to elucidate how STIM1- and Orai1-mediated calcium oscillations promote melanoma invasion

Mon Nov 24 00:00:00 UTC 2014

STIM1- and Orai1-mediated Ca(2+) oscillation orchestrates invadopodium formation and melanoma invasion.

The Journal of cell biology

Sun J,Lu F,He H,Shen J,Messina J,Mathew R,Wang D,Sarnaik AA,Chang WC,Kim M,Cheng H,Yang S

MA1-19451 was used in immunohistochemistry - paraffin section to elucidate how STIM1- and Orai1-mediated calcium oscillations promote melanoma invasion

Mon Nov 24 00:00:00 UTC 2014

STIM1-Ca(2+) signaling is required for the hypertrophic growth of skeletal muscle in mice.

Molecular and cellular biology

Li T,Finch EA,Graham V,Zhang ZS,Ding JD,Burch J,Oh-hora M,Rosenberg P

MA1-19451 was used in western blot to study hypertrophic murine skeletal muscle growth and the essential role of STIM1 regulated Ca(2+) signaling

Wed Aug 01 00:00:00 UTC 2012

Impaired Orai1-mediated resting Ca2+ entry reduces the cytosolic [Ca2+] and sarcoplasmic reticulum Ca2+ loading in quiescent junctophilin 1 knock-out myotubes.

The Journal of biological chemistry

Li H,Ding X,Lopez JR,Takeshima H,Ma J,Allen PD,Eltit JM

MA1-19451 was used in western blot to study the of Orai1 pathway in the control of plasmalemmal calcium permeability

Fri Dec 10 00:00:00 UTC 2010

Bioinformatics

Protein Aliases: stim1 {ECO:0000312|RGD:1306831}; STIM1L; Stromal interaction molecule 1

View more View less

Gene Aliases: D11S4896E; GOK; IMD10; SIM; STIM1; STRMK; TAM; TAM1

View more View less

UniProt ID: (Human) Q13586, (Mouse) P70302, (Rat) P84903

View more View less

Entrez Gene ID: (Human) 6786, (Mouse) 20866, (Rat) 361618

View more View less

Function(s)

protease binding calcium channel regulator activity calcium ion binding protein binding identical protein binding microtubule plus-end binding store-operated calcium channel activity metal ion binding

Process(es)

store-operated calcium entry detection of calcium ion cellular calcium ion homeostasis activation of store-operated calcium channel activity regulation of calcium ion transport enamel mineralization regulation of cardiac conduction transport ion transport cation transport calcium ion transport myotube differentiation calcium ion transmembrane transport regulation of store-operated calcium entry

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

STIM1 Monoclonal Antibody (CDN3H4)

STIM1 Antibody (MA1-19451) in ICC/IF

Product Details

MA1-19451

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!