Proteintech

STIM2 Polyclonal Antibody

STIM2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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STIM2 Antibody (21192-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using (Product # 21192-1-AP) (STIM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

STIM2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

21192-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

STIM2 Fusion Protein Ag15530 (525-674 aa encoded by BC146661)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.35 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: EKICGFQIA HNSGLPSLTS SLYSDHSWVV MPRVSIPPYP IAGGVDDLDE DTPPIVSQFP GTMAKPPGSL ARSSSLCRSR RSIVPSSPQP QRAQLAPHAP HPSHPRHPHH PQHTPHSLPS PDPDILSVSS CPALYRNEEE EEAIYFSAEK Q (525-674 aa encoded by BC146661)

Target Information

In T lymphocytes, the sole pathway for Ca++ entry following antigen-receptor binding is through store-operated Ca++-release-activated Ca++ (CRAC) channels. These channels are made up of the pore-forming subunit ORAI1 and the stromal interaction molecule 1 (STIM1), a protein that functions as a Ca++ sensor and activates the CRAC channels, migrating to the plasma membrane from endoplasmic reticulum (ER)-like sites which act as the Ca++ store. A related molecule, STIM2, acts to inhibit the STIM1-mediated store-operated Ca++ entry, and can form complexes with STIM1, suggesting they may play a coordinated role in controlling Ca++ entry. At least three isoforms of STIM2 are known to exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cholesterylation of Smoothened is a calcium-accelerated autoreaction involving an intramolecular ester intermediate.

Cell research

Hu A,Zhang JZ,Wang J,Li CC,Yuan M,Deng G,Lin ZC,Qiu ZP,Liu HY,Wang XW,Wei PC,He X,Zhao X,Qiu WW,Song BL

Tue Mar 01 00:00:00 UTC 2022

RGS10 physically and functionally interacts with STIM2 and requires store-operated calcium entry to regulate pro-inflammatory gene expression in microglia.

Cellular signalling

Wendimu MY,Alqinyah M,Vella S,Dean P,Almutairi F,Davila-Rivera R,Rayatpisheh S,Wohlschlegel J,Moreno S,Hooks SB

Thu Jul 01 00:00:00 UTC 2021

A short isoform of STIM1 confers frequency-dependent synaptic enhancement.

Cell reports

Ramesh G,Jarzembowski L,Schwarz Y,Poth V,Konrad M,Knapp ML,Schwär G,Lauer AA,Grimm MOW,Alansary D,Bruns D,Niemeyer BA

Tue Mar 16 00:00:00 UTC 2021

Deficiency of ER Ca²⁺ sensor STIM1 in AgRP neurons confers protection against dietary obesity.

Cell reports

Chen Z,Pan S,Yin K,Zhang Y,Yuan X,Wang S,Yang S,Shen Q,Tang Y,Li J,Wang Y,Lu Y,Zhang G

Tue Oct 19 00:00:00 UTC 2021

Resveratrol sensitizes A549 cells to irradiation damage via suppression of store-operated calcium entry with Orai1 and STIM1 downregulation.

Experimental and therapeutic medicine

Lele W,Lei L,Liting Q

Tue Jun 01 00:00:00 UTC 2021

Inhibiting store-operated calcium entry attenuates white matter secondary degeneration following SCI.

Neurobiology of disease

Orem BC,Partain SB,Stirling DP

Sun Mar 01 00:00:00 UTC 2020

Quantitative In Vivo Proteomics of Metformin Response in Liver Reveals AMPK-Dependent and -Independent Signaling Networks.

Cell reports

Stein BD,Calzolari D,Hellberg K,Hu YS,He L,Hung CM,Toyama EQ,Ross DS,Lillemeier BF,Cantley LC,Yates JR,Shaw RJ

Tue Dec 03 00:00:00 UTC 2019

The calcium channel proteins ORAI3 and STIM1 mediate TGF-β induced Snai1 expression.

Oncotarget

Bhattacharya A,Kumar J,Hermanson K,Sun Y,Qureshi H,Perley D,Scheidegger A,Singh BB,Dhasarathy A

Fri Jun 29 00:00:00 UTC 2018

The calcium channel proteins ORAI3 and STIM1 mediate TGF-β induced Snai1 expression.

Oncotarget

Bhattacharya A,Kumar J,Hermanson K,Sun Y,Qureshi H,Perley D,Scheidegger A,Singh BB,Dhasarathy A

Fri Jun 29 00:00:00 UTC 2018

STIM1 and STIM2 differently regulate endogenous Ca²⁺ entry and promote TGF-β-induced EMT in breast cancer cells.

Biochemical and biophysical research communications

Zhang S,Miao Y,Zheng X,Gong Y,Zhang J,Zou F,Cai C

Sat Jun 17 00:00:00 UTC 2017

STIM1 and STIM2 differently regulate endogenous Ca²⁺ entry and promote TGF-β-induced EMT in breast cancer cells.

Biochemical and biophysical research communications

Zhang S,Miao Y,Zheng X,Gong Y,Zhang J,Zou F,Cai C

Sat Jun 17 00:00:00 UTC 2017

STIM1 negatively regulates Ca²⁺ release from the sarcoplasmic reticulum in skeletal myotubes.

The Biochemical journal

Lee KJ,Woo JS,Hwang JH,Hyun C,Cho CH,Kim DH,Lee EH

Mon Jul 15 00:00:00 UTC 2013

STIM1 negatively regulates Ca²⁺ release from the sarcoplasmic reticulum in skeletal myotubes.

The Biochemical journal

Lee KJ,Woo JS,Hwang JH,Hyun C,Cho CH,Kim DH,Lee EH

Mon Jul 15 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: OTTHUMP00000123457; OTTHUMP00000123458; OTTHUMP00000216198; Stromal interaction molecule 2

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Gene Aliases: KIAA1482; STIM2

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UniProt ID: (Human) Q9P246, (Mouse) P83093

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Entrez Gene ID: (Human) 57620, (Mouse) 116873, (Rat) 117087

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Function(s)

calcium channel regulator activity calcium ion binding protein binding store-operated calcium channel activity metal ion binding

Process(es)

store-operated calcium entry cellular calcium ion homeostasis activation of store-operated calcium channel activity positive regulation of calcium ion transport calcium ion transmembrane transport transport ion transport calcium ion transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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STIM2 Polyclonal Antibody

STIM2 Antibody (21192-1-AP) in IHC (P)

Product Details

21192-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (MISC)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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