Invitrogen

STRO-1 Monoclonal Antibody (STRO-1), eBioscience™

STRO-1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 3

STRO-1 Antibody (14-6688-37) in ICC/IF

Immunocytochemistry on fixed MG-63 cells using 20 µg/mL Mouse IgM Isotype Control Purified (left) or 20 µg/mL Anti-STRO-1 Purified (right) followed by Anti-Mouse IgM FITC.Nuclei are counterstained with DAPI.

STRO-1 Antibody in Flow Cytometry (Flow)

Product Image Gallery

Product Details

14-6688-37

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Immunocytochemistry (ICC/IF)

20 µg/mL

View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/test

View 6 publications 6 publications

Product Specifications

Published species

Dog, Human, Rat

Host/Isotype

Mouse / IgM, lambda

Class

Monoclonal

Type

Antibody

Clone

STRO-1

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Storage conditions

4°C

Shipping conditions

Wet ice

AB_2865231

Product Specific Information

Description: The monoclonal antibody STRO-1 recognizes STRO-1, a bone marrow (immature) mesenchymal cell surface protein. Pluripotent stromal cells can give rise to several lineages: osteoblasts and chondrocytes, which form bone, adipocytes or hematopoietic cells. STRO-1+ cells when co-transplanted with hematopoietic stem cells have been shown to reduce the occurrence of graft versus host disease. In addition, in vitro cultures of Stro-1+ cells along with CD34+ cells give rise to CFUs (colony forming units) composed of hematopoietic precursors, establishing a supporting role of these mesenchymal cells in blood cell development.

The STRO-1 antibody has been reported to recognize both bovine and rat Stro-1 protein.

Applications Reported: This STRO-1 antibody has been reported for use in flow cytometric analysis, immunocytochemistry, and immunohistochemical staining of frozen tissue sections.

Applications Tested: This STRO-1 antibody has been tested by immunocytochemistry on methanol-fixed MG-63 cells and can be used at less than or equal to 20 µg/mL. The STRO-1 antibody has also been tested by flow cytometry on MG-63 cells and can be used at less than or equal to 0.5 µg/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

STRO-1 is a cell surface antigen expressed by stromal elements in human bone marrow. STRO-1 binds to approximately 10% of bone marrow mononuclear cells, greater than 95% of which are nucleated erythroid precursors, but does not react with committed progenitor cells (colony-forming unit granulocyte-macrophage (CFU-GM), erythroid bursts (BFU-E), and mixed colonies (CFU-Mix). From bone marrow cells, the frequency of fibroblast colony forming cells (CFU-F) is enriched approximately 100-fold in the STRO-1-positive/Glycophorin A-negative population than in the STRO-1-positive/Glycophorin A-positive population. When plated under long-term BM culture conditions, STRO-1-positive cells generate adherent cell layers containing multiple stromal cell types, including adipocytes, smooth muscle cells, and fibroblastic elements. A STRO-1-positive enriched subset of marrow cells is capable of differentiating into multiple mesenchymal lineages including hematopoiesis-supportive stromal cells with a vascular smooth muscle-like phenotype, adipocytes, osteoblasts and chondrocytes. STRO-1 is a valuable antibody for the identification, isolation and functional characterization of human bone marrow stromal cell precursors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Overexpression of OLIG2 and MYT1L Transcription Factors Enhance the Differentiation Potential of Human Mesenchymal Stem Cells into Oligodendrocytes.

Current issues in molecular biology

Fahim I,Ishaque A,Ramzan F,Shamsuddin SABA,Ali A,Salim A,Khan I

14-6688-82 was used in Immunocytochemistry to differentiate hUC-MSCs into oligodendrocyte-like cells, which is greatly facilitated by the oligo induction medium.

Sun May 07 00:00:00 UTC 2023

Engineering Stem Cell Recruitment and Osteoinduction via Bioadhesive Molecular Mimics to Improve Osteoporotic Bone-Implant Integration.

Research (Washington, D.C.)

Bai J,Ge G,Wang Q,Li W,Zheng K,Xu Y,Yang H,Pan G,Geng D

14-6688-82 was used in Immunohistochemistry-immunofluorescence to find that a rational E7/Y5 feeding ratio could lead to an optimal dual functionalization capable of not only significantly improving the biocompatibility of the implant but also enabling it to recruit endogenous BMSCs for colonization, proliferation, and osteogenic differentiation.

Wed Sep 28 00:00:00 UTC 2022

MicroRNA-149 suppresses osteogenic differentiation of mesenchymal stem cells via inhibition of AKT1-dependent Twist1 phosphorylation.

Cell death discovery

Fan J,Li S,Wang D

14-6688-82 was used in Immunohistochemistry to conclude that miR-149 silencing promoted osteogenic differentiation of MSCs by enhancing Twist1 degradation through AKT1 upregulation, representing a new method for bone repair treatment.

Mon Jan 10 00:00:00 UTC 2022

Induction of osteogenic differentiation of bone marrow stromal cells on 3D polyester-based scaffolds solely by subphysiological fluidic stimulation in a laminar flow bioreactor.

Journal of tissue engineering

Yamada S,Yassin MA,Schwarz T,Hansmann J,Mustafa K

14-6688-82 was used in Flow Cytometry to study osteogenesis initiation in synthetic environments as stimulated by a laminar flow bioreactor.

Tue Apr 02 00:00:00 UTC 2024

Compare features of human dental pulp cells cultured from pulp tissues with and without cryopreservation.

Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia

Yan M,Nada OA,Smeets R,Gosau M,Friedrich RE,Kluwe L

14-6688-82 was used in Flow Cytometry to show that cryopreserving fragmented dental pulp tissue provides a feasible option for saving pulp tissues as autologous cell sources for possible later application.

Mon Nov 01 00:00:00 UTC 2021

Bidirectional ephrinB2‑EphB4 signaling regulates the osteogenic differentiation of canine periodontal ligament stem cells.

International journal of molecular medicine

Zhu S,Liu Z,Yuan C,Lin Y,Yang Y,Wang H,Zhang C,Wang P,Gu M

14-6688-82 was used in Flow cytometry/Cell sorting to evaluate the effect of ephrinB2 gene‚transfected canine periodontal ligament stem cells (cPDLSCs) on the regulation of osteogenic differentiation.

Sun Mar 01 00:00:00 UTC 2020

Bidirectional ephrinB2‑EphB4 signaling regulates the osteogenic differentiation of canine periodontal ligament stem cells.

International journal of molecular medicine

Zhu S,Liu Z,Yuan C,Lin Y,Yang Y,Wang H,Zhang C,Wang P,Gu M

Sun Mar 01 00:00:00 UTC 2020

Expansion of Human Dental Pulp Cells In Vitro Under Different Cryopreservation Conditions.

In vivo (Athens, Greece)

Yan M,Nada OA,Kluwe L,Gosau M,Smeets R,Friedrich RE

14-6688 was used in Flow cytometry/Cell sorting to optimize the expansion of human dental pulp cells in vitro by exploring several cryopreservation methodologies.

Fri Jun 18 00:00:00 UTC 2021

Isolation and characterization of stem cells from differentially degenerated human lumbar zygapophyseal articular cartilage.

Molecular medicine reports

Xiao L,Xu S,Wang X,Jin Z,Wang J,Yang B,Xu H

14-6688 was used in Flow cytometry/Cell sorting to show stem cells with multilineage differentiation potential and clonal properties were identified in human LZAC, and these characteristics were more prominent in mildly degenerated as compared with severely degenerated articular cartilage.

Sat Dec 01 00:00:00 UTC 2018

Comprehensive characterization of chorionic villi-derived mesenchymal stromal cells from human placenta.

Stem cell research & therapy

Ventura Ferreira MS,Bienert M,Müller K,Rath B,Goecke T,Opländer C,Braunschweig T,Mela P,Brümmendorf TH,Beier F,Neuss S

Published figure using STRO-1 monoclonal antibody (Product # 14-6688-82) in Flow Cytometry

Mon Feb 05 00:00:00 UTC 2018

Bone marrow mesenchymal stem cells of the intrauterine growth-restricted rat offspring exhibit enhanced adipogenic phenotype.

International journal of obesity (2005)

Gong M,Antony S,Sakurai R,Liu J,Iacovino M,Rehan VK

14-6688 was used in Western Blotting to show markedly enhanced adipogenic molecular profile and increased cell proliferation of maternal food restriction bone marrow-derived mesenchymal stem cells.

Tue Nov 01 00:00:00 UTC 2016

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