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Invitrogen
SUPT4H1 Polyclonal Antibody
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SUPT4H1 Antibody (PA5-103308) in IHC (P)
Immunohistochemistry analysis of SUPT4H1 in mouse testis tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with SUPT4H1 polyclonal antibody (Product # PA5-103308) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Product Details
PA5-103308
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human SUPT4H1(Accession P63272), corresponding to amino acid residues N79-T117.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2852653
Product Specific Information
Antibody detects endogenous levels of total SPT4H.
Target Information
Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DRB sensitivity-inducing factor 14 kDa subunit; DRB sensitivity-inducing factor small subunit; DRB sensitivity-inducing factor small subunit 1; DSIF p14; DSIF small subunit; DSIF small subunit 1; hSPT4; small hSpt4 subunit; suppressor of Ty 4 homolog 1; Transcription elongation factor SPT4; Transcription elongation factor SPT4 1; Transcription elongation factor SPT4-A
Gene Aliases: AL022777; SPT4; SPT4H; Supt4a; SUPT4H; SUPT4H1; Supt4h1a
UniProt ID: (Human) P63272, (Mouse) P63271
Entrez Gene ID: (Human) 6827, (Mouse) 20922
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription elongation from RNA polymerase II promoter
negative regulation of DNA-templated transcription, elongation
positive regulation of DNA-templated transcription, elongation
negative regulation of transcription elongation from RNA polymerase II promoter
positive regulation of transcription from RNA polymerase II promoter
chromatin remodeling
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
positive regulation of viral transcription
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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