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Product Details
16511-1-AP
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
SUPT5H Fusion Protein Ag9728 (807-1087 aa encoded by BC024203)
Conjugate
Form
Liquid
Concentration
0.33 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Dry ice (international)
Product Specific Information
Immunogen sequence: PLHDGSRTP AQSGAWDPNN PNTPSRAEEE YEYAFDDEPT PSPQAYGGTP NPQTPGYPDP SSPQVNPQYN PQTPGTPAMY NTDQFSPYAA PSPQGSYQPS PSPQSYHQVA PSPAGYQNTH SPASYHPTPS PMAYQASPSP SPVGYSPMTP GAPSPGGYNP HTPGSGIEQN SSDWVTTDIQ VKVRDTYLDT QVVGQTGVIR SVTGGMCSVY LKDSEKVVSI SSEHLEPITP TKNNKVKVIL GEDREATGVL LSIDGEDGIV RMDLDEQLKI LNLRFLGKLL EA (807-1087 aa encoded by BC024203)
Target Information
Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DRB sensitivity-inducing factor 160 kDa subunit; DRB sensitivity-inducing factor large subunit; DSIF large subunit; DSIF p160; hSPT5; suppressor of Ty 5 homolog; Tat-cotransactivator 1 protein; Tat-CT1 protein; Transcription elongation factor SPT5
Gene Aliases: AL033283; AL033321; AU019549; SPT5; SPT5H; Supt5; SUPT5H; Tat-CT1
UniProt ID: (Human) O00267, (Mouse) O55201
Entrez Gene ID: (Human) 6829, (Mouse) 20924, (Rat) 308472
negative regulation of transcription from RNA polymerase II promoter
chromatin remodeling
DNA-templated transcription, elongation
transcription from RNA polymerase II promoter
transcription elongation from RNA polymerase II promoter
7-methylguanosine mRNA capping
cell cycle
response to organic substance
positive regulation of macroautophagy
negative regulation of DNA-templated transcription, elongation
positive regulation of DNA-templated transcription, elongation
single stranded viral RNA replication via double stranded DNA intermediate
positive regulation of transcription from RNA polymerase II promoter
positive regulation of viral transcription
negative regulation of mRNA polyadenylation
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
regulation of DNA-templated transcription, elongation
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